Abstract:
:The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced with mitomycin C and nalidixic acid. Transmission electron microscopy analysis exhibited tailed bacteriophages belonging to Myoviridae. A PCR assay targeting the capsid gene of the myoviruses proved phylogenetic position of induced phages. Nalidixic acid was poor inducer of prophages, however, it induced the OMV-like particles release. Size of OMVs depended on an antibiotic applied for phage induction and varied in the range of 30-80 and 120-200 nm. Inside some of them, tails of phages have been visible. Under conditions, inducing prophages, OMVs acted as the collectors of formed phage particles, using outer membrane receptors for phage detection (in this case, outer membrane siderophore receptor), and fulfilled therefore "a cleaning," as well as defensive functions, preventing bacteriophage spread outside population. This is the first description of myoviruses affiliated to K. intermedius, as well as outer membrane vesicles interaction with phages within this host.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Kharina A,Podolich O,Faidiuk I,Zaika S,Haidak A,Kukharenko O,Zaets I,Tovkach F,Reva O,Kremenskoy M,Kozyrovska Ndoi
10.1002/jobm.201400711subject
Has Abstractpub_date
2015-04-01 00:00:00pages
509-13issue
4eissn
0233-111Xissn
1521-4028journal_volume
55pub_type
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