Temperate bacteriophages collected by outer membrane vesicles in Komagataeibacter intermedius.

Abstract:

:The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced with mitomycin C and nalidixic acid. Transmission electron microscopy analysis exhibited tailed bacteriophages belonging to Myoviridae. A PCR assay targeting the capsid gene of the myoviruses proved phylogenetic position of induced phages. Nalidixic acid was poor inducer of prophages, however, it induced the OMV-like particles release. Size of OMVs depended on an antibiotic applied for phage induction and varied in the range of 30-80 and 120-200 nm. Inside some of them, tails of phages have been visible. Under conditions, inducing prophages, OMVs acted as the collectors of formed phage particles, using outer membrane receptors for phage detection (in this case, outer membrane siderophore receptor), and fulfilled therefore "a cleaning," as well as defensive functions, preventing bacteriophage spread outside population. This is the first description of myoviruses affiliated to K. intermedius, as well as outer membrane vesicles interaction with phages within this host.

journal_name

J Basic Microbiol

authors

Kharina A,Podolich O,Faidiuk I,Zaika S,Haidak A,Kukharenko O,Zaets I,Tovkach F,Reva O,Kremenskoy M,Kozyrovska N

doi

10.1002/jobm.201400711

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

509-13

issue

4

eissn

0233-111X

issn

1521-4028

journal_volume

55

pub_type

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