Abstract：
:The Mediterranean sponge Halichondria (Halichondria) panicea was explored as a novel matrix for the isolation of biosurfactant-producing bacteria. A total of 38 (out of 56) isolates gave a good response to the employed screening tests (e.g., stable emulsion detection, surface tension measurement, hemolytic activity, and blue agar plate assay) and were selected for further analyses. The thin layer chromatography revealed a possible glucidic composition of biosurfactants. Most promising strains, i.e., those able to produce stable emulsion with percentage higher than 30% and yellow spots on TLC plates, were affiliated to the genera Pseudovibrio, Acinetobacter, and Bacillus. The biosurfactant production by two isolates (i.e., Acinetobacter sp. SpN134 and Pseudovibrio sp. SpE85) was evaluated under different culture conditions, in terms of temperature, NaCl concentration, and pH. Surface tension reduction ability was more stable than the emulsification, and resulted differently influenced by salinity, temperature, and pH. Acinetobacter sp. SpN134 resulted particularly efficient and competitive if compared with other well-known biosurfactant producers. Data suggest that sponges may represent a promising matrix for the isolation of biosurfactant-producing bacteria, reinforcing the growing interest towards filter-feeding organisms as underexplored sources of specialized bacteria.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Rizzo C,Syldatk C,Hausmann R,Gerçe B,Longo C,Papale M,Conte A,De Domenico E,Michaud L,Lo Giudice Adoi
10.1002/jobm.201700669subject
Has Abstractpub_date
2018-06-01 00:00:00pages
532-542issue
6eissn
0233-111Xissn
1521-4028journal_volume
58pub_type
杂志文章abstract：:A culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate Cd, Co, Zn, Cr, and Hg up to 190, 525, 350, 935, and 370 μM, respectively. The isolate was identified as Lysinibacillus sphaericus by 16S rRNA gene sequencing. It bioaccumulated Cd, Co, and Zn, and reductively detoxified Cr...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201300308
更新日期：2015-03-01 00:00:00
abstract：:Cassava wastes--the peel and the root fibre were taken through various pretreatment procedures before being subjected to solid state fermentation with Trichoderma harzianum. Most of the pretreatment processes increased the cellulose and hemicellulose content of the cassava peel and fibre by as high as 155% while sulfu...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.3620360607
更新日期：1996-01-01 00:00:00
abstract：:Total cellular proteins and fatty acid composition profiles of mid-log phase cells of Salmonella enterica serovar Typhimurium grown at 8, 25, 37 or 42 °C were separated by 2D-PAGE and FAME analysis. Growth temperature associated protein expression can be grouped into 3 thermal classes which include proteins whose expr...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201000037
更新日期：2010-12-01 00:00:00
abstract：:Shigella adhesion to host cells is a transitional stage from an extracellular to an intracellular environment. However, the dynamic adaptations of Shigella during adhesion are poorly understood. To address this, we performed the first transcriptome analysis of Shigella flexneri 2457T during adhesion. A total of 1,757 ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201400414
更新日期：2015-02-01 00:00:00
abstract：:To understand the physiology of non-differentiating bacteria exposed to nutrient deprivation and stress, various approaches have been employed in combination with detailed analysis of protein synthesis pattern. In this study, separation of proteins from clarified cell extracts of Pseudomonas aeruginosa PAO1 grown unde...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.200390002
更新日期：2003-01-01 00:00:00
abstract：:Lyme disease, caused by Borrelia burgdorferi, is a focally endemic tick-transmitted zoonotic infection. In this study, the major factors underlying synonymous codon-related amino acid usage in the B. burgdorferi genome and bias in synonymous codon usage of the translation initiation region of coding sequences were ana...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201700652
更新日期：2018-05-01 00:00:00
abstract：:Sixteen Bacillus thuringiensis (Bt) strains were screened for their anti-insect, antibacterial and antifungal determinants by phenotypic tests and PCR targeting major insecticidal proteins and complements, chitinases, lactonases, beta-1,3-glucanases and zwittermicinA. Six strains had genes of at least two major insect...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.200800182
更新日期：2009-06-01 00:00:00
abstract：:A thermophilic, arsenate resistant bacterial strain was isolated from a geothermal field located in the area surrounding Monterotondo (Tuscany, Italy). Based on 16S rRNA gene analysis and recN comparisons the strain was identified as Geobacillus kaustophilus. Cells of the strain, designated A1, were rod-shaped, 2-3 μm...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201000314
更新日期：2011-08-01 00:00:00
abstract：:Aspergillus nidulans produces sterigmatocystin, a secondary metabolite mycotoxin, for the protection of its reproductive structures. Previous studies on grazing behavior of fungivore arthropods, regulation of sexual development, and secondary metabolite biosynthesis have revealed the association of sterigmatocystin bi...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201800020
更新日期：2018-07-01 00:00:00
abstract：:Histone-like nucleoid-structuring protein (H-NS) and integration host factor (IHF) are major nucleoid-associated proteins, and DnaA, a replication initiator, may also be related with nucleoid compaction. It has been shown that protein-dependent DNA compaction is related with many aspects of bacterial physiology, inclu...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201900497
更新日期：2020-02-01 00:00:00
abstract：:Many strains of Amycolatopsis, such as Amycolatopsis orientalis, A. balhimycina, and A. mediterranei, are important antibiotic producers. Three indigenous plasmids, pMEA100, pMEA300, and pA387, found in this genus have been sequenced. However, only some vectors based on pA387 have been widely applied in Amycolatopsis ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201400210
更新日期：2015-02-01 00:00:00
abstract：:The depolymerization of humic acids (HAs) obtained from low-rank coal (lignite) to fulvic acids (FAs) was investigated in a cell-free system (in vitro) using manganese peroxidase (MnP) from the white-rot fungus Clitocybula dusenii b11. MnP was produced in surface cultures of C. dusenii which were induced with manganes...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The possibility of associating starch degradation with bacterial beta-glucuronidase expression was examined. We proved that starving, in starch medium, amylase-negative Escherichia coli (M94) which has constitutive beta-glucuronidase greatly reduces (p < 0.01) its background activity, but the addition of both cell-fre...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:A potent β-CGTase producing bacterium ND1 has been isolated from sugarcane field soil in India. The biochemical, physiologicaland phylogenetic analyses based on 16S rRNA gene suggest that the isolate belongs to Bacillus cereus group. The enzyme β-CGTase produced from isolate ND1 catalyzes production of β-cyclodextrin ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201800390
更新日期：2019-02-01 00:00:00
abstract：:Patients with diabetes are considered a high-risk group involved with cerebral mucormycosis (CM). Due to the potential of Mucorales to invade sinuses and its rapid progression into orbit and retro-orbital areas and even brain, in most cases, CM is fatal in patients with diabetes. In the last few decades, mucormycosis ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章,评审
doi：10.1002/jobm.202000692
更新日期：2021-01-14 00:00:00
abstract：:Intracellular aminopeptidase synthesized by a soil strain of Pseudomonas sp. was purified 323-fold using the following procedure: saturation with ammonium sulfate, separation by preparative electrophoresis, anion-exchange chromatography and gel filtration chromatography. Molecular weight of the enzyme determined accor...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/1521-4028(200208)42:4<260::AID-JOBM260>3.0
更新日期：2002-01-01 00:00:00
abstract：:Every organism can be characterized by the amino acid composition of its proteome. So far it was assumed that these compositions are determined by the GC content of the DNA or, in some cases, by extreme lifestyles, like thermophily or halophily. Here, we focussed our analysis on eight amino acids, each of which is enc...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201100067
更新日期：2012-04-01 00:00:00
abstract：:The aim of this work was to study the catabolic pathway of the pollutant 2,4,6-trichlorophenol in Ralstonia eutropha JMP134. 2,6-dichlorohydroquinone was detected as transient intermediate. Enzymatic transformations of 6-chlorohydroxyquinol to 2-chloromaleylacetate, and of this compound to maleylacetate were detected ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/1521-4028(200008)40:4<243::AID-JOBM243>3.0
更新日期：2000-01-01 00:00:00
abstract：:From seawater and the intestines of benthonic organisms collected from the Beagle Channel, Argentina, 230 marine bacteria were isolated. Cultivable bacteria were characterized and classified as psychrotolerant, whereas few isolates were psychrophiles. These isolates were capable of producing proteases at 4 and 15 °C u...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201000413
更新日期：2011-12-01 00:00:00
abstract：:Swainsonine is a polyhydroxylated indolizidine alkaloid having anticancer, antimetastatic, antiproliferative and immunomodulatory activities and also potential therapeutic applications against AIDS. In the present study, ten isolates of M. anisopliae were screened and enzyme assayed for the production of swainsonine i...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201100255
更新日期：2012-10-01 00:00:00
abstract：:An outbreak of serious mortality among the cultured cobia Rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in July of 2000 in Taiwan. A non-motile strain CP1 was isolated from kidney and/or liver on tryptic soy agar and/or bra...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.200310301
更新日期：2003-01-01 00:00:00
abstract：:Membrane-attached nucleoids were isolated from E. coli and separated from proteins by 2 M NaCl. Disintegration of such nucleoids by ultrasound and subsequent centrifugation resulted in the formation of two fractions: a sediment (fraction I) and a supernatant (fraction II). The protein:DNA ratio of fraction I was equal...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.3620260408
更新日期：1986-01-01 00:00:00
abstract：:The phylogeny of the latest recognized domain, Archaea, is still complicated and it is largely based on environmental sequences. A culture independent molecular phylogenetic analysis revealed high Archaea diversity in a terrestrial hot spring, village Varvara, Bulgaria. A total of 35 archaeal operational taxonomic uni...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201000160
更新日期：2011-04-01 00:00:00
abstract：:The impact of increasing Cd2+ exposure on the quality and quantity of siderophores produced by a plant growth promoting Pseudomonas fulva strain was tested to gain insight into the degree of change. P. fulva was cultured in the increasing concentrations of Cd2+ (0, 0.5, 1.0, 2.0 mM). The secreted siderophores were sep...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201800034
更新日期：2018-07-01 00:00:00
abstract：:A study was conducted to examine the prevalence of brucellosis (in animal farms) in the vicinity of Islamabad and Rawalpindi. A total of 170 milk samples were collected randomly from several farmhouses. The collected milk samples were initially screened by a Brucella selective medium. The bacterial isolates grown on t...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201900454
更新日期：2020-03-01 00:00:00
abstract：:In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical pur...
journal_title：Journal of basic microbiology
pub_type： 杂志文章,评审
doi：10.1002/jobm.200800292
更新日期：2009-02-01 00:00:00
abstract：:Differentiation commitment is one of the most complex mechanisms to study in biological science. One of the model systems used for understanding differentiation complexity is heterocyst development in cyanobacteria. Cyanobacteria have the capability of biological nitrogen fixation due to highly differentiated heterocy...
journal_title：Journal of basic microbiology
pub_type： 杂志文章,评审
doi：10.1002/jobm.202000266
更新日期：2020-09-01 00:00:00
abstract：:The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.200410403
更新日期：2004-01-01 00:00:00
abstract：:Studies were carried out to screen twelve strains of Lentinus edodes for their efficiency to grow on toxic agro-industrial residues of coffee industry in solid state cultivation (SSC). Based on best mycelial growth (7.57 mm/day) and biomass production (48.78 mg/plate in 12 days at 24 degrees C) in coffee husk extract ...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/1521-4028(200007)40:3<187::AID-JOBM187>3.0
更新日期：2000-01-01 00:00:00
abstract：:The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...
journal_title：Journal of basic microbiology
pub_type： 杂志文章
doi：10.1002/jobm.201200189
更新日期：2013-09-01 00:00:00
