Abstract:
:Campylobacter jejuni is an important human food-borne intestinal pathogen, however relatively little is known about its mechanisms of pathogenesis or pathogen-host interactions. To monitor changes in gene expression and glycan binding of C. jejuni within a common avian host, an immunomagnetic separation technique (IMS) was utilised to directly isolate infecting C. jejuni 81116 from a chicken host. An average of 10(5) cells/g was re-isolated from chicken caecal samples by IMS technique. The in vivo passaged strains were used successfully in evaluation of carbohydrate binding through the use of a glycan array and were further suitable for transcriptome analysis. The glycan microarray analysis demonstrated differences in binding to negatively charged glycans of laboratory grown strains of C. jejuni compared with strains isolated after in vivo passage. The in vivo passaged strains showed marked up-regulation of chemotaxis receptors and toxin genes. The optimised Campylobacter IMS technique described in this study allowed isolation directly from an animal host. Changes in gene expression and glycan binding at an in vivo level can also be identified by using this method.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
King RM,Day CJ,Hartley-Tassell LE,Connerton IF,Tiralongo J,McGuckin MA,Korolik Vdoi
10.1002/jobm.201100466subject
Has Abstractpub_date
2013-03-01 00:00:00pages
240-50issue
3eissn
0233-111Xissn
1521-4028journal_volume
53pub_type
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