Abstract:
:A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24 delta EcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Jurský F,Hostinová Edoi
10.1002/jobm.3620300419subject
Has Abstractpub_date
1990-01-01 00:00:00pages
301-4issue
4eissn
0233-111Xissn
1521-4028journal_volume
30pub_type
杂志文章abstract::To discuss the microbial structure that develops during the course of compost burials of polyvinyl alcohol (PVA)-based material and analyze the effect of the degree of PVA alcoholysis on the PVA-degrading ability of each strain. Two bacterial strains exhibiting a high level of PVA-degrading ability and two mixed cultu...
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journal_title:Journal of basic microbiology
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