DNA sequences from Saccharomycopsis fibuligera capable of autonomous replication in Saccharomyces cerevisiae.

Abstract:

:A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24 delta EcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones were recovered with Escherichia coli, mapped, hybridized with total DNA of S. fibuligera and tested to mitotical stability in Saccharomyces cerevisiae. Experiments suggested the existence of DNA sequences from dimorphic Saccharomycopsis fibuligera with ARS activity in Saccharomyces cerevisiae.

journal_name

J Basic Microbiol

authors

Jurský F,Hostinová E

doi

10.1002/jobm.3620300419

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

301-4

issue

4

eissn

0233-111X

issn

1521-4028

journal_volume

30

pub_type

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