Tracing of neuronal connections with cholera toxin subunit B: light and electron microscopic immunohistochemistry using monoclonal antibodies.


:Subunit B of cholera toxin was used as a tracer substance in the central nervous system after being injected into various brain regions, mainly somatosensory relay structures. The tracer was localized with an immunoperoxidase technique, using monoclonal antibodies raised in mouse hybridomas. This method, which is applicable in both light and electron microscopic studies, is characterized by high contrast between specific labeling and unspecific background activity. It yields excellent retrograde labeling of the dendritic tree and is thus suitable for studying the neuronal cytoarchitecture and, on the ultrastructural level, the synaptic organization of identified projection neurons.


J Neurosci Methods


Ericson H,Blomqvist A




Has Abstract


1988-07-01 00:00:00














  • The image-combining computer microscope--an interactive instrument for morphometry of the nervous system.

    abstract::The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Glaser EM,Tagamets M,McMullen NT,Van der Loos H

    更新日期:1983-05-01 00:00:00

  • Application of triphasic pulses with adjustable phase amplitude ratio (PAR) for cochlear ECAP recording: II. recovery functions.

    abstract::Triphasic electrical stimulation pulses with an adjustable phase amplitude ratio (PAR) can reduce stimulus artifacts in electrically evoked compound actions potentials (ECAPs) recording in the cochlea (see companion paper Bahmer and Baumann, submitted for publication). The present study describes the application of tr...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Bahmer A,Baumann U

    更新日期:2012-03-30 00:00:00

  • A novel version of the 8-arm radial maze: effects of cerebral ischemia on learning and memory.

    abstract::A novel version of the 8-arm radial maze task was developed to quantify spatial learning and memory in rats subjected to transient cerebral ischemia (TCI) using the 4-VO model. This maze uses the rat's natural behavior of avoiding open, illuminated areas, and preference for a darkened, enclosed shelter. Ischemic rats ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Paganelli RA,Benetolli A,Lima KC,Cestari-Junior LA,Favero Filho LA,Milani H

    更新日期:2004-01-15 00:00:00

  • Use of surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) to study protein expression in a rat model of cocaine withdrawal.

    abstract::Surface enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF MS) is an analytical technology for proteomic analysis that combines chromatography and mass spectrometry. At present, this technology is most commonly being exploited for the simultaneous measurement of numerous proteins in serum...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Geng T,Seitz PK,Thomas ML,Xu B,Soman KV,Kurosky A,Luxon BA,Cunningham KA

    更新日期:2006-11-15 00:00:00

  • Measurement of phase gradients in the EEG.

    abstract::Previous research has shown that spatio-temporal waves in the EEG are generally of long spatial wavelength and form smooth patterns of phase gradients at particular time-samples. This paper describes a method to measure smooth phase gradients of long spatial wavelength in the EEG. The method depends on the global patt...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Alexander DM,Trengove C,Wright JJ,Boord PR,Gordon E

    更新日期:2006-09-30 00:00:00

  • Cleaning MEG artifacts using external cues.

    abstract::Using EEG, ECoG, MEG, and microelectrodes to record brain activity is prone to multiple artifacts. The main power line (mains line), video equipment, mechanical vibrations and activities outside the brain are the most common sources of artifacts. MEG amplitudes are low, and even small artifacts distort recordings. In ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Tal I,Abeles M

    更新日期:2013-07-15 00:00:00

  • Rectification of the EMG is an unnecessary and inappropriate step in the calculation of Corticomuscular coherence.

    abstract::Corticomuscular coherence (CMC) estimation is a frequency domain method used to detect a linear coupling between rhythmic activity recorded from sensorimotor cortex (EEG or MEG) and the electromyogram (EMG) of active muscles. In motor neuroscience, rectification of the surface EMG is a common pre-processing step prior...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: McClelland VM,Cvetkovic Z,Mills KR

    更新日期:2012-03-30 00:00:00

  • Comparison of different quantification methods to determine hippocampal damage after cerebral ischemia.

    abstract:BACKGROUND:Experimental stroke studies use multiple techniques to evaluate histopathological damage. Unfortunately, sensitivity and reproducibility of these techniques are poorly characterized despite pivotal influence on results. METHOD:The present study compared several quantification methods to differentiate betwee...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wang J,Jahn-Eimermacher A,Brückner M,Werner C,Engelhard K,Thal SC

    更新日期:2015-01-30 00:00:00

  • Neurotransmitter receptor autoradiography in immunohistochemically identified neurons.

    abstract::A method for the simultaneous visualization of neurotransmitter receptor binding sites and immunohistochemically characterized cells is described using in vitro sections of rat striatum. The striatum provides a rich neurochemical environment in which to examine muscarinic acetylcholine receptor relations to peptide-re...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ariano MA,Kenny SL

    更新日期:1985-10-01 00:00:00

  • Subcellular distribution of delta-opioid receptors in the rat spinal cord: an approach using a three-dimensional reconstruction of confocal series of immunolabelled neurons.

    abstract::Using a specific rat monoclonal anti-idiotypic antibody we have examined the subcellular distribution of -opioid receptors in various neuronal subtypes of the rat spinal cord. The immunofluorescence was detected with a confocal microscope and in some cases serial images were processed for a three-dimensional (3-D) rec...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mailly P,Gastard M,Cupo A

    更新日期:1999-02-01 00:00:00

  • Gene expression patterns in rat dentate granule cells: comparison between fresh and fixed tissue.

    abstract::RNA from brain tissue (in particular human brain) can often only be extracted from fixed material. As brain tissue is very heterogeneous with regard to cell type, it is important to obtain RNA from small samples of identified cells. The aim of this study was (a) to generate expression profiles from small yet homogeneo...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Qin Y,Heine VM,Karst H,Lucassen PJ,Joëls M

    更新日期:2003-12-30 00:00:00

  • Precision rodent whisker stimulator with integrated servo-locked control and displacement measurement.

    abstract::We developed a high speed voice coil based whisker stimulator that delivers precise deflections of a single whisker or group of whiskers in a repeatable manner. The device is miniature, quiet, and inexpensive to build. Multiple stimulators fit together for independent stimulation of four or more whiskers. The system c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Walker JL,Monjaraz-Fuentes F,Pedrow CR,Rector DM

    更新日期:2011-03-15 00:00:00

  • Indo-1 measurements of intracellular free calcium in the hippocampal slice: complications of labile NADH fluorescence.

    abstract::The use of fluorescent calcium indicator, Indo-1, was evaluated for measuring changes in intracellular free calcium during electrical stimulation and anoxia in hippocampal slices. Fluorescence was measured from slices illuminated with brief (3 ns) light pulses (337 nm wavelength) from a nitrogen laser. This method of ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Sick TJ,Rosenthal M

    更新日期:1989-05-01 00:00:00

  • Adenoviral vector-mediated gene transfer and neurotransplantation: possibilities and limitations in grafting of the fetal rat suprachiasmatic nucleus.

    abstract::Several studies have reported on the use of primary neural cells transduced by adenoviral vectors as donor cells in neurotransplantation. In the present investigation, we examined whether adenoviral vector-mediated gene transfer could be used to introduce and express a foreign gene in solid neural transplants of fetal...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: van Esseveldt KE,Liu R,Hermens WT,Verhaagen J,Boer GJ

    更新日期:1997-01-01 00:00:00

  • Electrical stimulation of excitable tissue: design of efficacious and safe protocols.

    abstract::The physical basis for electrical stimulation of excitable tissue, as used by electrophysiological researchers and clinicians in functional electrical stimulation, is presented with emphasis on the fundamental mechanisms of charge injection at the electrode/tissue interface. Faradaic and non-Faradaic charge transfer m...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Merrill DR,Bikson M,Jefferys JG

    更新日期:2005-02-15 00:00:00

  • Iterative expert-in-the-loop classification of sleep PSG recordings using a hierarchical clustering.

    abstract:BACKGROUND:The classification of sleep signals is a subjective and time consuming task. A large number of automatic classifiers have been published in the past decade but a sleep community has no strong confidence to use them in clinical practice and still remains using a standard manual scoring according standardized ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Gerla V,Kremen V,Macas M,Dudysova D,Mladek A,Sos P,Lhotska L

    更新日期:2019-04-01 00:00:00

  • Pulsed linear acceleration as a vestibular stimulus in electrophysiological investigations.

    abstract::A simple method of generating precisely defined pulsed linear acceleration stimuli was developed and used to study vestibular responses to cranial acceleration. Electromechanical shakers were coupled to a stimulus platform. The platform, in turn, was used to couple stimuli to the skull. Movements were controlled by a ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Jones TA,Schiltz T

    更新日期:1989-03-01 00:00:00

  • Utilizing commercial microwave for rapid and effective immunostaining.

    abstract::There is an accumulating literature demonstrating the application of microwaves across a wide spectrum of histological techniques. Although exposure to microwaves for short periods resulted in substantial acceleration of all procedures this technique still is not adopted widely. In part, this may be due to concerns ov...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Owens K,Park JH,Kristian T

    更新日期:2013-09-30 00:00:00

  • A simple computer-based method for performing and analyzing intracranial self-stimulation experiments in rats.

    abstract::Intracranial self-stimulation (ICSS) in the rat is a useful tool for studying the importance of various brain monoamines in positive reinforcement. The effects of compounds interacting with dopaminergic neurotransmission is measurable by studying the changes of reward thresholds. By computerisation of the analysis of ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kling-Petersen T,Svensson K

    更新日期:1993-05-01 00:00:00

  • Time-frequency component analyser and its application to brain oscillatory activity.

    abstract::Currently, event-related potential (ERP) signals are analysed in the time domain (ERP technique) or in the frequency domain (Fourier analysis and variants). In techniques of time-domain and frequency-domain analysis (short-time Fourier transform, wavelet transform) assumptions concerning linearity, stationarity, and t...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ozdemir AK,Karakaş S,Cakmak ED,Tüfekçi DI,Arikan O

    更新日期:2005-06-30 00:00:00

  • Enrichment of differentiated hNT neurons and subsequent analysis using flow-cytometry and xCELLigence sensing.

    abstract:BACKGROUND:Human neurons (hNT neurons), obtained from the NTera2/D1 precursor cell line, are highly valued by many neuroscientists as isolation of adult human primary neuronal cells continues to elude us. hNT neurons are generated by differentiation of the NT2 precursors for a period of 4 weeks followed by 2 weeks of m...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: MacDonald C,Unsworth CP,Graham ES

    更新日期:2014-04-30 00:00:00

  • An integrative transcranial magnetic stimulation mapping technique using non-linear curve fitting.

    abstract::The aim of this study is to develop a new simple method for analyzing one-dimensional transcranial magnetic stimulation (TMS) mapping studies in humans. Motor evoked potentials (MEP) were recorded from the abductor pollicis brevis (APB) muscle during stimulation at nine different positions on the scalp along a line pa...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kohl AS,Conforto AB,Z'Graggen WJ,Kaelin-Lang A

    更新日期:2006-10-30 00:00:00

  • Stereotaxic operation in the neonatal rat; a novel and simple procedure.

    abstract::Since no simple method for standardized positioning of very young rats in a stereotaxic device has been described in the literature, a new method for stereotaxic placement in the neonatal rat was developed, which only requires a simple body mould of the rat pup, and a minor adaptation of a rabbit stereotaxic apparatus...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hoorneman EM

    更新日期:1985-07-01 00:00:00

  • The advantages and limitations of permanent or reversible deactivation techniques in the assessment of neural function.

    abstract::This review considers the different forms of reversible deactivation and how they differ from each other and from more conventional permanent deactivation methods. The different methods of chemical and cooling reversible inactivation and the limitations of each are discussed. Finally, future directions in the quest to...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Lomber SG

    更新日期:1999-01-01 00:00:00

  • Destruction of a sub-population of cortical neurones by suicide transport of volkensin, a lectin from Adenia volkensii.

    abstract::A method for the destruction of a sub-population of neocortical pyramidal neurones is described. The technique uses unilateral striatal injections of volkensin, a toxic lectin from Adenia volkensii, which undergoes retrograde axonal transport from the site of injection to destroy subcortically projecting pyramidal cel...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Pangalos MN,Francis PT,Pearson RC,Middlemiss DN,Bowen DM

    更新日期:1991-11-01 00:00:00

  • Generation of a neurodegenerative disease mouse model using lentiviral vectors carrying an enhanced synapsin I promoter.

    abstract:BACKGROUND:Certain inherited progressive neurodegenerative disorders, such as spinocerebellar ataxia (SCA), affect neurons in large areas of the central nervous system (CNS). The selective expression of disease-causing and therapeutic genes in susceptible regions and cell types is critical for the generation of animal ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Matsuzaki Y,Oue M,Hirai H

    更新日期:2014-02-15 00:00:00

  • A practical device for histological fixative procedures that limits formaldehyde deleterious effects in laboratory environments.

    abstract::Formaldehyde vapor levels were evaluated in a histological fixative laboratory and environments where anatomical pieces treated with this fixative are stored or handled. Formaldehyde concentration in the air may reach values up to 9 times higher than that established (0.3 ppm) by the international official surveillanc...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Nogueira MI,Barbieri C,Vieira R,Marques ER,Moreno JE

    更新日期:1997-03-01 00:00:00

  • Combined fluorescent in situ hybridization and immunofluorescence: limiting factors and a substitution strategy for slide-mounted tissue sections.

    abstract::The simultaneous localization of several anatomical markers is often required to understand and analyze the organization of complex brain nuclei or identify neuronal networks recruited during a specific biological stimulus. Gathering such information is usually achieved by the combined detection of both mRNA and prote...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Nehmé B,Henry M,Mouginot D

    更新日期:2011-03-30 00:00:00

  • Wide-area measurement-based supervision of the cerebral venous hemodynamic in a novel rat model.

    abstract:BACKGROUND:Traumatic brain injury (TBI) includes primary and secondary injuries, while monitoring intracranial pressure (ICP) and cerebral blood flow (CBF) is conducive to improve the prognosis of patients. However, the function of cerebral venous in this process is still unclear. NEW METHOD:An acute epidural hematoma...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wang W,Mu S,Xue L,Liang S,Li Z,Wang S

    更新日期:2019-12-01 00:00:00

  • Reliability of the microdialysis pump CMA 107 under hyperbaric conditions.

    abstract:OBJECTIVE:Microdialysis measurements of extracellular substances under hyperbaric conditions were manifold used in several investigations. However, to our knowledge there is no analysis, which verified the applicability of microdialysis pumps under hyperbaric conditions. Thus, a goal of this study was to investigate th...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Henze D,Köthe L,Scharf A,Clausen T

    更新日期:2007-08-30 00:00:00