Abstract:
:The concentration of free calcium within the lumen of the endoplasmic reticulum ([Ca2+]L) fluctuates between 100 and 1000 microM. High [Ca2+]L provides an electro-driving force for Ca2+ release and supports high Ca2+ diffusion rate within the endoplasmic reticulum lumen. Fluctuations in [Ca2+]L also regulate numerous chaperones, responsible for postranslational protein processing. Thus, [Ca2+]L integrates various signalling events and establishes a link between fast signalling, associated with the endoplasmic reticulum Ca2+release/uptake, and long-lasting adaptive responses relying primarily on the regulation of protein synthesis. This paper overviews modern approaches for the direct monitoring of [Ca2+]L which rely on three classes of low-affinity Ca2+ probes: ER-targeted aequorin, synthetic fluorescent Ca2+ dyes and GFP-based ER-targeted Ca2+ probes. These techniques, especially as applied to neurones, may substantially widen our appreciation of the endoplasmic reticulum as a universal signalling organelle.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Solovyova N,Verkhratsky Adoi
10.1016/s0165-0270(02)00300-xsubject
Has Abstractpub_date
2002-12-31 00:00:00pages
1-12issue
1eissn
0165-0270issn
1872-678Xpii
S016502700200300Xjournal_volume
122pub_type
杂志文章,评审abstract::Morphometric estimation of neuronal processes is currently laborious and time-consuming, since the individual processes (axons and dendrites) have to be traced manually. In order to facilitate the measurement of cellular processes, we have tested a series of parameters derived from the contour and the convex hull of a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00165-9
更新日期:1998-01-31 00:00:00
abstract::Implantable neural micro-electrode arrays have the potential to restore lost sensory or motor function to many different areas of the body. However, the invasiveness of these implants often results in scar tissue formation, which can have detrimental effects on recorded signal quality and longevity. Traditional histol...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.06.001
更新日期:2013-08-15 00:00:00
abstract::Analysis of the antigenic components of synaptic membranes presents several problems caused by detergents required to solubilize the proteins. These problems involve detection of the reaction between antibodies and proteins in the detergent extract as well as characterization of the individual antigens after further p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(80)90058-8
更新日期:1980-04-01 00:00:00
abstract::By extending established technology, we have developed a relatively simple in situ preparation from juvenile rat that overcomes some technical restrictions present in vivo (e.g. need for anaesthesia, mechanical instability for intracellular recording and control over the extracellular milieu). The in situ preparation ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.01.011
更新日期:2006-09-15 00:00:00
abstract::Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the rapid degradation of fatty acid amides such as the endocannabinoid anandamide. Inhibition of FAAH activity has been suggested as a therapeutic approach for the treatment of chronic pain, depression and anxiety, through local activation of the cannabin...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.10.006
更新日期:2007-03-30 00:00:00
abstract::Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(92)90061-h
更新日期:1992-06-01 00:00:00
abstract::Estimating the degree of synchrony or reliability between two or more spike trains is a frequent task in both experimental and computational neuroscience. In recent years, many different methods have been proposed that typically compare the timing of spikes on a certain time scale to be optimized by the analyst. Here,...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.05.031
更新日期:2007-09-15 00:00:00
abstract:BACKGROUND:Cerebral air emboli occur as a complication of invasive medical procedures. The sensitivity of cerebral monitoring methods for the detection of air emboli is not known. This study investigates the utility of electroencephalography and non-invasively measured cerebral oxygen saturation in the detection of int...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.03.009
更新日期:2014-05-15 00:00:00
abstract::Electroneurograms (ENGs) from the vagus, splanchnic and pancreatic nerves innervating the pancreas of a dog, were recorded with chronically implanted silicone multi-electrode circular cuffs in an intact pancreas and in a pancreas partly disabled with alloxan. The cuffs contained 33 platinum electrodes (0.6x1.5 mm) arr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00462-9
更新日期:2001-12-15 00:00:00
abstract::So far, the double-magnetic induction (DMI) method has been successfully applied to record eye movements from head-restrained humans, monkeys and cats. An advantage of the DMI method, compared to the more widely used scleral search coil technique, is the absence of vulnerable lead wires on the eye. A disadvantage, how...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.08.012
更新日期:2007-02-15 00:00:00
abstract::We demonstrate herein that the standard 'Trolox equivalent antioxidant capacity' (TEAC) assay, typically utilized to quantify total antioxidant levels within plasma, can also be utilized for tissue homogenates. Normal mice and transgenic mice lacking apolipoprotein E were subjected to a diet including iron as a generi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(03)00028-1
更新日期:2003-05-30 00:00:00
abstract::A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90040-3
更新日期:1986-05-01 00:00:00
abstract:BACKGROUND:Dilated brain perivascular spaces (PVSs) are found to be associated with many conditions, including aging, dementia, and Alzheimer's disease (AD). Conventionally, PVS assessment is mainly based on subjective observations of the number, size and shape of PVSs in MR images collected at clinical field strengths...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.09.001
更新日期:2015-12-30 00:00:00
abstract::Motorized linear slides used as micromanipulators for biological use suffer from 3 problems: vibration at low speed, poor ergonomic design of the controller and slow coarse positioning. These problems are not hard to solve by minor modifications to the controller and the slides. ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90040-x
更新日期:1989-06-01 00:00:00
abstract::Licking behavior in rodents is widely used to determine fluid consumption in various behavioral contexts and is a typical example of rhythmic movement controlled by internal pattern-generating mechanisms. The measurement of licking behavior by commercially available instruments is based on either tongue protrusion int...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.10.023
更新日期:2006-06-15 00:00:00
abstract:BACKGROUND:Zebrafish larvae have a high potential as model system to replace rodents, especially in screening and drug discovery applications. However, an experimental setup to deliver mild electrical stimuli with simultaneous high throughput behavioural tracking has not yet been described. NEW METHOD:A new tool was d...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.03.002
更新日期:2018-05-01 00:00:00
abstract:INTRODUCTION:Transcranial magnetic stimulation (TMS) is used for assessing the excitability of cortical neurons and corticospinal pathways by determining the subject-specific motor threshold (MT). However, the MT is dependent on the TMS instrumentation and exhibits large variation. We hypothesized that between-subject ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.10.004
更新日期:2012-01-30 00:00:00
abstract::A novel, inexpensive apparatus (locometer) was designed to measure behavioral activity of rats including gross locomotor activity, rearing, and structural characteristics of movement patterns (i.e. fractal dimension). Measurements were done in 3 dimensions at a resolution of approximately 1 cm. To test the sensitivity...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00145-7
更新日期:1995-04-01 00:00:00
abstract::Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90053-t
更新日期:1993-10-01 00:00:00
abstract:BACKGROUND:Functional magnetic resonance imaging is well suited to link neural population activation with movement parameters of complex natural arm movements. However, currently existing MR-compatible arm tracking devices are not constructed to measure arm joint movement parameters of unrestricted movements. Therefore...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.01.015
更新日期:2017-03-15 00:00:00
abstract::Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00133-6
更新日期:1996-02-01 00:00:00
abstract::A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00061-5
更新日期:1995-11-01 00:00:00
abstract::Sensory and motor pathways in the central nervous system (CNS) of macaque monkeys were visualized by anterograde or retrograde axonal transport of wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) reacted with the chromagen tetramethylbenzidine (TMB), or by the use of anterograde degeneration after specific ablati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00117-y
更新日期:1995-02-01 00:00:00
abstract::The hypothesis that the aortic depressor nerve (ADN) from spontaneously hypertensive rats (SHR) does not show the expected correlation between myelin sheath area and the axonal diameter of myelinated fibers detected in normotensive rat myelinated fibers was tested by means of regression analysis. Proximal and distal s...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.04.007
更新日期:2005-10-30 00:00:00
abstract::The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00328-4
更新日期:2001-03-30 00:00:00
abstract::Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00015-8
更新日期:2002-04-15 00:00:00
abstract::A method for quantitative estimation of sensory neuron sensitivity to small sinusoidal stimuli in the presence of sizable background drift (in the stimulus or response) was developed. The performance of the method was tested by analyzing the responses of 17 muscle spindle primary (Ia) afferent neurons to concomitant s...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)90175-9
更新日期:1994-08-01 00:00:00
abstract:BACKGROUND:The frequency-following response (FFR) is a scalp-recorded electrophysiological potential reflecting phase-locked activity from neural ensembles in the auditory system. The FFR is often used to assess the robustness of subcortical pitch processing. Due to low signal-to-noise ratio at the single-trial level, ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.08.010
更新日期:2017-11-01 00:00:00
abstract::A method for studying the process of regeneration in the frog sciatic nerve is presented. The outgrowth of sensory axons was determined by assaying the distribution of axonally transported radioactive proteins by fluorography of whole-mount nerve preparations. After labelling, the nerves were fixed at their in vivo le...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90004-x
更新日期:1986-03-01 00:00:00
abstract::An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a spec...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(80)90035-7
更新日期:1980-10-01 00:00:00