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A decerebrate, artificially-perfused in situ preparation of rat: utility for the study of autonomic and nociceptive processing.

Abstract：

:By extending established technology, we have developed a relatively simple in situ preparation from juvenile rat that overcomes some technical restrictions present in vivo (e.g. need for anaesthesia, mechanical instability for intracellular recording and control over the extracellular milieu). The in situ preparation is decerebrate and artificially perfused via the left ventricle with a colloid containing solution. It exhibits an eupneic pattern of respiratory motor activity and demonstrates numerous somatic and visceral reflexes including those evoked by stimulation of the tail, hindlimbs, bladder, baroreceptors and peripheral chemoreceptors. We have employed this preparation to allow recordings from multiple sympathetic motor outflows such as thoracic and lumbar chain, inferior cardiac, splanchnic, renal and adrenal nerves. We show that the sympathetic motor discharge shows strong respiratory modulation and exhibits pronounced reflex modulation indicating intact communication between the periphery, the brainstem and the spinal cord. Further, we have made extracellular and whole cell recordings from neurones in the spinal cord, demonstrating good mechanical stability. The decerebrate, artificially-perfused rat (DAPR) provides a powerful methodology with which to study peripheral and central control of the autonomic nervous system with many of the benefits of an in vitro environment.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Pickering AE,Paton JF

doi

10.1016/j.jneumeth.2006.01.011

subject

Has Abstract

pub_date

2006-09-15 00:00:00

pages

260-71

issue

2

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(06)00048-3

journal_volume

155

pub_type

杂志文章

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