Abstract:
:A method by which muscles, nerves, and a section of spinal cord from a turtle can be stably maintained in vitro for many days has been developed. Tests of the viability of central and neuromuscular synapses, and the histochemical properties of muscle fibers have indicated that the preparation is viable and shows little decrement in function over a period of 3-5 days. The system allows continuous access to the muscles, nerves, and the spinal cord, as well as a controlled external environment.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Lennard PR,Boss V,Shirley KAdoi
10.1016/0165-0270(89)90029-0subject
Has Abstractpub_date
1989-08-01 00:00:00pages
165-9issue
2eissn
0165-0270issn
1872-678Xpii
0165-0270(89)90029-0journal_volume
29pub_type
杂志文章abstract::The biotin derivative, N-(2-aminoethyl) biotinamide hydrochloride, or Neurobiotin, has been shown recently to be a useful marker for intracellular and anterograde tracing. The properties of Neurobiotin as a tracer were further examined in this study by making pressure injections into different regions of the cerebral ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(92)90121-s
更新日期:1992-01-01 00:00:00
abstract::Most techniques used for the study of the fiber connectivity in the central nervous system produce results which are visualized in the conventional light microscope or fluorescence microscope. Although in some cases this may be sufficient, often proof is necessary that fibers which enter a particular brain area indeed...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00300-9
更新日期:2000-11-15 00:00:00
abstract:BACKGROUND:Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the ana...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.02.011
更新日期:2016-05-01 00:00:00
abstract::We assessed the potential of using multielectrode arrays (MEAs) to investigate several physiological properties of the calyx of Held synapse in the medial nucleus of the trapezoid body of gerbil. Due to the large size of the synapse, it became widely employed in studies on synaptic mechanisms. Electrical stimulation a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.07.015
更新日期:2008-09-30 00:00:00
abstract:BACKGROUND:Planarians, like rodents, instinctively spend more time in dark versus light environments when given a choice. This behavioral phenomenon is called negative phototaxis, which may reflect defensive responding related to an anxiety-like phenotype. NEW METHOD:We propose a planarian light/dark test, designated ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.10.010
更新日期:2018-01-01 00:00:00
abstract::Characterizing the structure and electrophysiological properties of single neurons is essential for understanding how individual cells contribute to the function of neuronal networks. Following intra-cellular recording from neurons in acute brain slices, the structure of the recorded cell has typically been examined b...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00341-2
更新日期:2003-02-15 00:00:00
abstract:BACKGROUND:Tinnitus, head and ear noise, is due to maladaptive plastic changes in auditory and associated neural networks. Tinnitus has been traditionally managed through the use of sound to passively mask or facilitate habituation to tinnitus, a process that may take 6-12 months. NEW METHOD:A game-based perceptual tr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.04.002
更新日期:2015-07-15 00:00:00
abstract:BACKGROUND:Local shape complexity can be biologically meaningful as a marker of disease, trauma, or change in brain structure over time. Fractal dimensionality (FD) is currently the dominant measure of local shape complexity used in neuroimaging but its limitations are not well understood. NEW METHOD:Elliptical Fourie...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.05.009
更新日期:2019-07-15 00:00:00
abstract:BACKGROUND:Magnetoencephalography (MEG) and Electroencephalography (EEG) are noninvasive techniques to study the electrophysiological activity of the human brain. Thus, they are well suited for real-time monitoring and analysis of neuronal activity. Real-time MEG/EEG data processing allows adjustment of the stimuli to ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.03.020
更新日期:2018-06-01 00:00:00
abstract::Accurate estimates of the total number of neurons located in the wall of the gut are essential for studies of the enteric nervous system (ENS). Though several stains and antibodies are used routinely as pan-neuronal markers, controversies of relative sensitivity and completeness have been difficult to resolve, at leas...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.10.004
更新日期:2004-02-15 00:00:00
abstract::In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Im...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00211-9
更新日期:2000-06-30 00:00:00
abstract:BACKGROUND:Peripheral neuropathies affect approximately 20 million people in the United States and often stem from other chronic conditions, such as diabetes. In vitro methodologies to facilitate the understanding and treatment of these disorders often lack the cellular and functional complexity required to accurately ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.05.003
更新日期:2018-07-15 00:00:00
abstract::A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.09.016
更新日期:2010-12-15 00:00:00
abstract::According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.11.010
更新日期:2009-03-30 00:00:00
abstract::The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(84)90021-9
更新日期:1984-04-01 00:00:00
abstract::Oxidative stress is one of the mechanisms which may be important in the pathogenesis of Parkinson's disease. In the current study, the effects of 6-hydroxydopamine (6-OHDA) perfusion on hydroxyl radical formation in the mouse striatum were investigated using the in vivo salicylate trapping microdialysis technique. The...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.07.004
更新日期:2011-11-15 00:00:00
abstract::Biolistics, also known as particle-mediated gene transfer, has been used as an effective, method to transfect primary neurons in cultured slices when all other methods have proven unsuccessful. Most of these uses have provided qualitative or semi-quantitative data based on visual assays such as immunohistochemistry. I...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00117-4
更新日期:1998-10-01 00:00:00
abstract::This paper describes a computer program, named ASAP (audio sequencing and presentation), that runs on IBM PC compatible hardware with a Microsoft Windows operating system and has been specially designed for facilitating auditory neuroimaging studies. The program's main characteristics are: (1) the ability to generate ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00249-1
更新日期:2000-08-15 00:00:00
abstract::We describe a semi-automated tracking system for insect motion based on commercially available high-speed video cameras and freely available software. We use it to collect detailed three-dimensional kinematic information from female crickets performing free walking phonotaxis towards a calling song stimulus. We mark t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.011
更新日期:2012-01-15 00:00:00
abstract::The paired motor unit analysis provides in vivo estimates of the magnitude of persistent inward currents (PIC) in human motoneurons by quantifying changes in the firing rate (ΔF) of an earlier recruited (reference) motor unit at the time of recruitment and derecruitment of a later recruited (test) motor unit. This stu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.03.018
更新日期:2011-05-15 00:00:00
abstract:BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108634
更新日期:2020-04-15 00:00:00
abstract::We have examined the stability and sources of variation within the nociceptive model of rat hind paw withdrawal from an under-glass radiant stimulus (Hargreaves et al., 1988) using a system where stimulus intensity and floor temperature can be controlled and reproducibly changed. The current study demonstrates that: (...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00097-6
更新日期:1997-10-03 00:00:00
abstract::A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibitio...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(82)90015-2
更新日期:1982-07-01 00:00:00
abstract::Modern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2020.108594
更新日期:2020-01-13 00:00:00
abstract::A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00173-3
更新日期:1999-02-01 00:00:00
abstract::A reliable technique for purification of crude tissue extracts and analysis for TRH by HPLC, TLC, radioimmunoassay and bioassay is presented. We conclude that authentic TRH is present throughout the mammalian brain and accounts for much of the TRH-like immunoreactivity in several peripheral organs, but that it is not ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(81)90002-9
更新日期:1981-12-01 00:00:00
abstract::We examined topographic variations in nonlinear measures based on scalp voltages, which were generated by two simulated current dipoles each placed in a different hemisphere of a spherical volume conductor (three-shell model). Dipole dynamics were that of a three-torus and the x-component of the Lorenz-system and scal...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00188-6
更新日期:2000-05-15 00:00:00
abstract:BACKGROUND:The sound fed to a loudspeaker may significantly differ from that reaching the ear of the listener. The transformation from one to the other consists of spectral distortions with strong dependence on the relative locations of the speaker and the listener as well as on the geometry of the environment. With th...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.08.025
更新日期:2018-11-01 00:00:00
abstract:BACKGROUND:The widespread use of data-driven methods, such as independent component analysis (ICA), for the analysis of functional magnetic resonance imaging data (fMRI) has enabled deeper understanding of neural function. However, most popular ICA algorithms for fMRI analysis make several simplifying assumptions, thus...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.03.012
更新日期:2016-05-01 00:00:00
abstract::Combining multiple linear univariate features in one feature space and classifying the feature space using machine learning methods could predict epileptic seizures in patients suffering from refractory epilepsy. For each patient, a set of twenty-two linear univariate features were extracted from 6 electroencephalogra...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.03.019
更新日期:2013-07-15 00:00:00