Abstract：
:I describe here the successful visualization of interhemispheric callosal connections in the live mammalian cortex. The development of this method was prompted by the finding that fluorescent tracer labeling of groups of cortical neurons, when done under optimal conditions, is sufficiently intense to be visible even in the whole brain preparation. The new approach could provide a useful tool for enhanced precision in localizing cortical modules in vivo. In a typical experiment, rats had their left cortical hemisphere extensively injected with the fluorescent tract-tracer bisBenzimide (BB). After appropriate survival, the right cortical hemisphere was illuminated with UV light and the fluorescing callosal pattern could be discerned under the network of blood vessels even with the unaided eye. The pattern, although diffuse, was grossly similar to the pattern of callosal connections as seen in flattened, sectioned cortex. Features that could be discerned were: the main callosal band straddling the lateral border of area 17, several rings and bands in extrastriate areas 18a and 18b, and a major band straddling the lateral border of area 3. The vitally visualized callosal pattern was used to guide injections of either wheat germ agglutinin conjugated to HRP (WGA-HRP) or rhodamine-labeled microspheres (RLM) into precisely localized sites in occipital cortex. There were numerous instances of doubly labeled neurons stained both with BB and WGA-HRP or RLM, suggesting that uptake of BB combined with UV exposure did not hinder the ability of stained neurons to take up and transport a second tracer. It is suggested that vital tract tracing be used as a tool for enhanced precision in studies of cortical connectivity.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Malach Rdoi
10.1016/0165-0270(88)90137-9subject
Has Abstractpub_date
1988-10-01 00:00:00pages
225-38issue
3eissn
0165-0270issn
1872-678Xpii
0165-0270(88)90137-9journal_volume
25pub_type
杂志文章abstract：BACKGROUND:To optimize survival evaluation of Schwann cells (SCs) in vivo, we tested fluorescent labeling of the nucleus as an improved method of tracking and counting the transplanted SCs at sciatic nerve injury sites in rodents. We also investigated if co-administering cells with the glial growth factor Neuregulin-1 ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.08.009
更新日期：2016-11-01 00:00:00
abstract：:Holding a slipping object in hand requires adjustment of grip forces. The aim of the study was to develop a method for measuring the temporal and spatial distribution of grip forces during the holding of a slipping object in the hand. A special grip rod with a measuring film containing 200 resistor-based pressure sens...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.07.014
更新日期：2007-11-30 00:00:00
abstract：:Studies of the neural control of movement often require or benefit from long-term recording of EMG activity from large numbers of muscles involved in a particular motor task. While chronic recording of EMG activity has been described in a number of previous monkey studies, the number of muscles recorded has been somew...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00155-2
更新日期：2000-03-15 00:00:00
abstract：:The process of peripheral nerve regeneration has been modeled using 5 populations of mathematical variables to represent the biological activities of Wallerian degeneration, fibrin matrix development, Schwann cell activity, elongating neurites, and neovascularization. The mathematical model provided simulations of ner...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00222-3
更新日期：1995-08-01 00:00:00
abstract：UNLABELLED:Lobeline is a potential smoking cessation drug with affinity for the α4β2 nicotinic acetylcholine receptor and may inhibit the blood-brain barrier (BBB) amine transporter. The goal of this work was to use PET imaging to evaluate the effects of lobeline on the kinetic properties of [(18)F]nifene in the rat br...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.01.018
更新日期：2013-04-15 00:00:00
abstract：:The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00328-4
更新日期：2001-03-30 00:00:00
abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00262-5
更新日期：2002-12-15 00:00:00
abstract：BACKGROUND:Single unit recording in behaving nonhuman primates is widely used to study the primate central nervous system. However, certain questions cannot be addressed without recording large numbers of neurons simultaneously. Multiple 96-electrode probes can be implanted at one time, but certain problems must be ove...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.07.001
更新日期：2017-09-01 00:00:00
abstract：:We have developed and characterized a method for the rapid autoradiographic determination of receptor sites for the non-competitive NMDA receptor antagonist, MK-801, using an iodinated form of the compound, (+)-3-[125I]-iodo-MK-801. The binding site was shown to exhibit those criteria necessary for its definition as a...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90137-g
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.10.009
更新日期：2014-01-15 00:00:00
abstract：BACKGROUND:Neurite outgrowth is a metric widely used to assess the success of in vitro neural stem cell differentiation or neuron reprogramming protocols and to evaluate high-content screening assays for neural regenerative drug discovery. However, neurite measurements are tedious to perform manually, and there is a pa...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.03.013
更新日期：2017-05-01 00:00:00
abstract：:The cerebellar Purkinje neuron (PN) serves as an important model in studies of neuronal development in the mammalian central nervous system. Dissociated PN preparations maintained in an in-vitro environment with simplified cellular and biochemical conditions can facilitate molecular analyses of neuronal development. H...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00323-x
更新日期：2000-12-15 00:00:00
abstract：:Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90053-t
更新日期：1993-10-01 00:00:00
abstract：:A procedure is described by which the solid silver chromate precipitate present in Golgi-impregnated neurons is reduced by a photographic developer into metallic silver grains or particles. The remaining silver chromate is subsequently removed, and the fine structure and synaptic relationships of the impregnated neuro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90023-7
更新日期：1983-04-01 00:00:00
abstract：:Attempts to correlate the amount of bilateral brain injury with behavioral changes can be complicated if the lesions are bilaterally unsymmetrical in volume. Further complications are introduced if unilateral lesions are ineffective in producing behavioral changes. An index, W%, is proposed which is based on the produ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90046-3
更新日期：1984-11-01 00:00:00
abstract：:A method for the simultaneous visualization of neurotransmitter receptor binding sites and immunohistochemically characterized cells is described using in vitro sections of rat striatum. The striatum provides a rich neurochemical environment in which to examine muscarinic acetylcholine receptor relations to peptide-re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90061-5
更新日期：1985-10-01 00:00:00
abstract：BACKGROUND:The brain generates signals in a wide frequency range (∼2840 Hz). Existing magnetoencephalography (MEG) methods typically detect brain activity in a median-frequency range (1-70 Hz). The objective of the present study was to develop a new method to utilize the frequency signatures for source imaging. NEW ME...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.10.007
更新日期：2015-01-15 00:00:00
abstract：:We present an efficient algorithm for solving the one-dimensional cable equation in the Laplace (frequency) domain for an arbitrary linear membrane. This method, a reformulation and extension of the geometrical calculus developed by Butz and Cowan (1974), solves for the transfer impedance between any two points in a b...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90015-9
更新日期：1985-02-01 00:00:00
abstract：:Vertex analysis defines dichotomous networks in terms of the relative frequencies of pendant (Vp) and nodal (Vd) vertices. Va, Vb and Vc comprise the 3 classes of Vd which connect 2, 1 and 0 Vp respectively. The vertex ratio (VR) of Va/Vb may be used to define topology since Va and Vc approach equality in large networ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90118-4
更新日期：1986-10-01 00:00:00
abstract：BACKGROUND:Military personnel and civilians living in areas of armed conflict have increased risk of exposure to blast overpressures that can cause significant hearing loss and/or brain injury. The equipment used to simulate comparable blast overpressures in animal models within laboratory settings is typically very la...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.01.009
更新日期：2015-03-15 00:00:00
abstract：:A method is described for achieving gaze control in cats, by rewarding them for pushing against a lever with their tongue, only when a light-emitting diode (LED) was flickering. Since the state of the LED was changed sequentially from OFF to ON to flickering and the duration of flickering was short, the cats were requ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90036-9
更新日期：1980-10-01 00:00:00
abstract：:Frequency analyses of EEG data yield large data sets, which are high-dimensional and have to be evaluated statistically without a large number of false positive statements. There exist several methods to deal with this problem in multiple comparisons. Knowing the number of true hypotheses increases the power of some m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.013
更新日期：2008-05-15 00:00:00
abstract：BACKGROUND:The complexity of analyzing eye-tracking signals increases as eye-trackers become more mobile. The signals from a mobile eye-tracker are recorded in relation to the head coordinate system and when the head and body move, the recorded eye-tracking signal is influenced by these movements, which render the subs...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.09.005
更新日期：2016-12-01 00:00:00
abstract：:Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possibl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.07.010
更新日期：2012-10-15 00:00:00
abstract：:Capsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximately 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately or 2 months later by...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00164-8
更新日期：2000-02-15 00:00:00
abstract：:Gamma band synchronization has drawn increasing interest with respect to its potential role in neuronal encoding strategy and behavior in awake, behaving animals. However, contamination of these recordings by power line noise can confound the analysis and interpretation of cortical local field potential (LFP). Existin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.10.029
更新日期：2011-01-15 00:00:00
abstract：:Glass plates coated with transparent thin film conductors of indium-tin oxide (ITO), 100 nm thick and 10 microns wide, have been successfully used to record spike potentials from neuronal monolayer cultures. The material is non-toxic to mammalian spinal neurons and is stable under warm culture medium. Laser-deinsulate...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90105-0
更新日期：1985-11-01 00:00:00
abstract：:The electrochemical technique of differential pulse voltammetry (DPV) with micro-biosensors has been used for a number of years to monitor in vivo and in situ changes in the extracellular concentration of cerebral ascorbic acid, as well as that of the metabolites of dopamine (DA) and serotonin (5-HT). We have recently...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90094-t
更新日期：1992-05-01 00:00:00
abstract：:We have designed a new device for head fixation of behaving monkeys. The fixation device consists of a duralumin head ring mounted with four screw holders. It is firmly fixed to the animal's skull with four stainless steel screw pins. The head ring is then attached to a primate chair in any desirable position and angl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.07.003
更新日期：2005-02-15 00:00:00
abstract：:Several studies have reported on the use of primary neural cells transduced by adenoviral vectors as donor cells in neurotransplantation. In the present investigation, we examined whether adenoviral vector-mediated gene transfer could be used to introduce and express a foreign gene in solid neural transplants of fetal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00131-8
更新日期：1997-01-01 00:00:00
