Abstract：
BACKGROUND:Military personnel and civilians living in areas of armed conflict have increased risk of exposure to blast overpressures that can cause significant hearing loss and/or brain injury. The equipment used to simulate comparable blast overpressures in animal models within laboratory settings is typically very large and prohibitively expensive. NEW METHOD:To overcome the fiscal and space limitations introduced by previously reported blast wave generators, we developed a compact, low-cost blast wave generator to investigate the effects of blast exposures on the auditory system and brain. RESULTS:The blast wave generator was constructed largely from off the shelf components, and reliably produced blasts with peak sound pressures of up to 198dB SPL (159.3kPa) that were qualitatively similar to those produced from muzzle blasts or explosions. Exposure of adult rats to 3 blasts of 188dB peak SPL (50.4kPa) resulted in significant loss of cochlear hair cells, reduced outer hair cell function and a decrease in neurogenesis in the hippocampus. COMPARISON TO EXISTING METHODS:Existing blast wave generators are typically large, expensive, and are not commercially available. The blast wave generator reported here provides a low-cost method of generating blast waves in a typical laboratory setting. CONCLUSIONS:This compact blast wave generator provides scientists with a low cost device for investigating the biological mechanisms involved in blast wave injury to the rodent cochlea and brain that may model many of the damaging effects sustained by military personnel and civilians exposed to intense blasts.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Newman AJ,Hayes SH,Rao AS,Allman BL,Manohar S,Ding D,Stolzberg D,Lobarinas E,Mollendorf JC,Salvi Rdoi
10.1016/j.jneumeth.2015.01.009subject
Has Abstractpub_date
2015-03-15 00:00:00pages
82-92eissn
0165-0270issn
1872-678Xpii
S0165-0270(15)00010-2journal_volume
242pub_type
杂志文章abstract：:Information concerning the location and distribution of presynaptic neurotransmitter release sites within anatomically labeled axons would be of value for a large number of studies in functional anatomy, development, and plasticity. Here we report a method for localizing presynaptic sites within identified arbors of i...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00145-4
更新日期：2000-01-15 00:00:00
abstract：:Microdialysis is a useful tool for administering drugs into localized regions of brain tissue, but the diffusion of drugs from the probe has not been systematically examined. Lidocaine (10%) and tetrodotoxin (TTX, 10 microM), drugs typically used in neural inactivation studies, were infused through a microdialysis pro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00348-4
更新日期：2001-02-15 00:00:00
abstract：:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.010
更新日期：2009-03-30 00:00:00
abstract：:Electrical potentials recorded extracellularly from the sinus gland of the isopod, Oniscus asellus, were stored on video tape with the aid of a digital-audio (DA) processor and a video cassette recorder (VCR). The DA processor transforms the analog signal to digital pulses of equal amplitude and converts these pulses ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90058-5
更新日期：1985-10-01 00:00:00
abstract：:Chronic spinal catheterization via an atlanto-occipital puncture (CAO) has been widely used to study the effects of drugs on spinal nociceptive mechanisms, but this method is associated with spinal cord damage that may change the efficacy of spinally injected analgesics. Using a slight modification of the method of St...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00197-3
更新日期：2003-10-15 00:00:00
abstract：:MR connectomics is an emerging framework in neuro-science that combines diffusion MRI and whole brain tractography methodologies with the analytical tools of network science. In the present work we review the current methods enabling structural connectivity mapping with MRI and show how such data can be used to infer ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2010.01.014
更新日期：2010-12-15 00:00:00
abstract：:The use of c-fos protein (Fos) immunocytochemistry as a metabolic marker for tracing neuroanatomical connections, seizure pathways and sites of action of neuroactive drugs is discussed in this report. Fos immunocytochemistry will be very useful for these purposes providing that a number of potential problems are recog...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(89)90150-7
更新日期：1989-09-01 00:00:00
abstract：:Sleep is an innate behavior conserved in all animals and, in vertebrates, is regulated by neuronal circuits in the brain. The conventional techniques of forward and reverse genetics have enabled researchers to investigate the molecular mechanisms that regulate sleep and arousal. However, functional interrogation of ge...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2018.10.016
更新日期：2019-03-15 00:00:00
abstract：:A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.03.021
更新日期：2004-09-30 00:00:00
abstract：BACKGROUND:Local shape complexity can be biologically meaningful as a marker of disease, trauma, or change in brain structure over time. Fractal dimensionality (FD) is currently the dominant measure of local shape complexity used in neuroimaging but its limitations are not well understood. NEW METHOD:Elliptical Fourie...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.05.009
更新日期：2019-07-15 00:00:00
abstract：:Most spinal cord injury research is undertaken using in vivo animal models but the extensive care associated with spinalized animals, inherent variability between animals, and complex surgeries makes alternative models especially valuable. Here we present a novel ex vivo model that enables culture of intact post-natal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.07.012
更新日期：2010-09-30 00:00:00
abstract：:Testing electrophysiological recording equipments is an important task in multi-channel extracellular in vivo electrophysiology. In this paper, a miniature, battery powered multi-channel electrophysiological signal-generator (ESG) is described that was designed for this purpose. The device is based on a Xilinx CPLD (C...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.05.017
更新日期：2007-09-15 00:00:00
abstract：:Glass plates coated with transparent thin film conductors of indium-tin oxide (ITO), 100 nm thick and 10 microns wide, have been successfully used to record spike potentials from neuronal monolayer cultures. The material is non-toxic to mammalian spinal neurons and is stable under warm culture medium. Laser-deinsulate...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90105-0
更新日期：1985-11-01 00:00:00
abstract：BACKGROUND:Peripheral neuropathies affect approximately 20 million people in the United States and often stem from other chronic conditions, such as diabetes. In vitro methodologies to facilitate the understanding and treatment of these disorders often lack the cellular and functional complexity required to accurately ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.05.003
更新日期：2018-07-15 00:00:00
abstract：:In this report we describe the development of an in vitro preparation of the mudpuppy (Necturus maculatus) used to investigate locomotion in walking vertebrates. The preparation consists of the first 5 segments of the cervical spinal cord and the attached forelimb. The preparation is bathed in a cooled (15 degrees C) ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90143-2
更新日期：1992-04-01 00:00:00
abstract：:Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.03.013
更新日期：2009-06-15 00:00:00
abstract：:In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Im...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00211-9
更新日期：2000-06-30 00:00:00
abstract：:There is an accumulating literature demonstrating the application of microwaves across a wide spectrum of histological techniques. Although exposure to microwaves for short periods resulted in substantial acceleration of all procedures this technique still is not adopted widely. In part, this may be due to concerns ov...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.07.002
更新日期：2013-09-30 00:00:00
abstract：:A common approach to test the time-response of carbon fibre microelectrodes entails the use of a flow injection system which allows delivery of a bolus of test compound in the flow stream to a detector electrode. The introduced bolus is distorted from its original form by convective and dispersive forces during the pe...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00086-7
更新日期：1998-10-01 00:00:00
abstract：BACKGROUND:Independent component analysis (ICA) has been widely used for blind source separation in the field of medical imaging. However, despite of previous substantial efforts, the stability of ICA components remains a critical issue which has not been adequately addressed, despite numerous previous efforts. Most cr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109013
更新日期：2020-12-11 00:00:00
abstract：BACKGROUND:Since task related neurons cannot be specifically targeted during surgery, a critical decision to make is to select which neurons are task-related when performing data analysis. Including neurons unrelated to the task degrade decoding accuracy and confound neurophysiological results. Traditionally, task-rela...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.02.020
更新日期：2015-04-30 00:00:00
abstract：:Several studies have reported on the use of primary neural cells transduced by adenoviral vectors as donor cells in neurotransplantation. In the present investigation, we examined whether adenoviral vector-mediated gene transfer could be used to introduce and express a foreign gene in solid neural transplants of fetal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00131-8
更新日期：1997-01-01 00:00:00
abstract：:Our laboratory has previously characterized a high affinity choline uptake system (HAChUS) in Limulus tissues and synaptosomes. We report here on the characterization of the HAChUS in synaptosomes prepared selectively from central nervous system tissues shown to be enriched for presumed cholinergic functions; namely t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90166-w
更新日期：1991-07-01 00:00:00
abstract：:The limited success of immunogold labeling for pre-embedding immunocytochemistry of neuronal antigens is largely attributed to poor penetration of large (5-20 nm) colloidal gold particles. We examined the applicability of using silver intensification of 1 nm colloidal gold particles non-covalently bound to goat anti-r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90015-8
更新日期：1990-08-01 00:00:00
abstract：:Quantifying the connectivity between arbitrary surface patches in the human brain cortex can be used in studies on brain function and to characterize clinical diseases involving abnormal connectivity. Cortical regions of human brain in their natural forms can be represented in surface formats. In this paper, we presen...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneumeth.2011.02.017
更新日期：2011-04-30 00:00:00
abstract：BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.015
更新日期：2015-05-15 00:00:00
abstract：:Microwire array electrodes are important in multi-site, multiple single-unit recording experiments. A simple method is described herein for the construction of microwire arrays consisting of evenly spaced insulated microwires in either horizontal or vertical orientations. Several key steps in the fabrication of a good...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00235-8
更新日期：2003-12-30 00:00:00
abstract：:The coupling of Ca(2+) influx to synaptic vesicle (SV) recycling in nerve terminals is essential for neurotransmitter release and thus neuronal communication. Both of these parameters have been monitored using fluorescent reporter dyes such as fura-2 and FM1-43 in single central nerve terminals. However, their simulta...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.09.012
更新日期：2007-03-15 00:00:00
abstract：:We present a simple and efficient method for expressing cDNAs in Purkinje neurons (PNs) present in heterogeneous mouse cerebellar cultures. The method combines the transfection of freshly dissociated cerebellar cells via nucleofection with the use of novel expression plasmids containing a fragment of the L7 (Pcp2) gen...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.06.006
更新日期：2011-09-15 00:00:00
abstract：:We report an original method for implanting bone marrow stem cells within the spinal cord parenchyma. This method was used for the experimental treatment of patients diagnosed with amyotrophic lateral sclerosis. The methodology is reproducible and devoid of major complications even in patients showing significant spin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.06.014
更新日期：2010-08-30 00:00:00
