Abstract：
:Glass plates coated with transparent thin film conductors of indium-tin oxide (ITO), 100 nm thick and 10 microns wide, have been successfully used to record spike potentials from neuronal monolayer cultures. The material is non-toxic to mammalian spinal neurons and is stable under warm culture medium. Laser-deinsulated recording craters that expose 100 mu m2 of ITO yield impedances of 8-10 M omega at 1 kHz with noise levels of 40 muV. Conventional gold plating of the craters reduces these impedances to below 3 M omega. The material is easily etchable and sputtered glass plates of high quality are commercially available at relatively low cost. The high light transmittance of ITO makes the conductors essentially invisible and allows unobstructed observation of circuit components in monolayer cultures. The introduction of ITO as a thin film microelectrode material should accelerate the construction of high density recording patterns that could exceed 400 microelectrodes per mm2.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Gross GW,Wen WY,Lin JWdoi
10.1016/0165-0270(85)90105-0subject
Has Abstractpub_date
1985-11-01 00:00:00pages
243-52issue
3eissn
0165-0270issn
1872-678Xpii
0165-0270(85)90105-0journal_volume
15pub_type
杂志文章abstract：:Electrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale. Despite great progress in imaging technologies and sensor proteins, no...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
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abstract：:The development of high-resolution retinal prostheses fabricated from silicon wafers presents an interesting problem: how to electrically bridge the space between the flat silicon wafer and the curved retinal surface. One potential "bridge" is a microwire glass electrode. In this paper we present our results in evalua...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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abstract：BACKGROUND:In order to evaluate nerve regeneration in clinically relevant hindlimb surgical paradigms not feasible in fluorescent mice models, we developed a rat that expresses green fluorescent protein (GFP) in neural tissue. METHODS:Transgenic Sprague-Dawley rat lines were created using pronuclear injection of a tra...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.04.004
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1991-08-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.09.002
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.04.006
更新日期：2016-07-15 00:00:00
abstract：:The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.027
更新日期：2012-01-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90166-w
更新日期：1991-07-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90056-8
更新日期：1988-06-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.024
更新日期：2015-06-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.06.004
更新日期：2010-08-15 00:00:00
abstract：:Electrophysiological recording provides important evidence for positive identification of many neurons in gastropods. We describe a technique which combines intracellular recording and injection of a persistent, non-fluorescent dye (Fast Green) with subsequent histofluorescence treatment using a modification of the wh...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90036-6
更新日期：1985-08-01 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2011-04-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90009-3
更新日期：1992-09-01 00:00:00
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更新日期：2001-05-30 00:00:00
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pub_type： 杂志文章
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更新日期：2021-01-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2005-03-15 00:00:00
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更新日期：2001-10-15 00:00:00
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更新日期：1986-04-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1993-05-01 00:00:00
abstract：BACKGROUND:Single unit recording in behaving nonhuman primates is widely used to study the primate central nervous system. However, certain questions cannot be addressed without recording large numbers of neurons simultaneously. Multiple 96-electrode probes can be implanted at one time, but certain problems must be ove...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2017-09-01 00:00:00
