Abstract：
:Immunodetection techniques are dependent on enzyme-protein conjugates for the visualisation of antigen-antibody complexes. One of the most widely used is the avidin-biotin-peroxidase complex (ABC) method. The present study demonstrates that direct treatment of goldfish and salamander retinal sections with ABC, followed by an incubation with the chromogenic substrate 3,3-diaminobenzidine tetrahydrochloride (DAB) and H2O2, manifested a punctate staining pattern across the neural retinae, presumably through binding of avidin to endogenous biotin. Incubation with a primary antiserum against biotin followed by immunoprocessing with the peroxidase--anti-peroxidase (PAP) method showed a pattern similar to the punctuate framework as detected with solo ABC-treated sections. Moreover, the ABC-DAB/H2O2 mediated pattern corresponded to the spatial orientation of Müller cells as identified by GFAP immunostaining. These findings indicate the presence of endogenous biotin in Müller cells and calls for caution in the application of the ABC method in immunotechniques in retinal research.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Bhattacharjee J,Nunes Cardozo B,Kamphuis W,Kamermans M,Vrensen GFdoi
10.1016/s0165-0270(97)00114-3subject
Has Abstractpub_date
1997-11-07 00:00:00pages
75-82issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(97)00114-3journal_volume
77pub_type
杂志文章abstract：:Combining multiple linear univariate features in one feature space and classifying the feature space using machine learning methods could predict epileptic seizures in patients suffering from refractory epilepsy. For each patient, a set of twenty-two linear univariate features were extracted from 6 electroencephalogra...
journal_title：Journal of neuroscience methods
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