Abstract：
:To facilitate the combination of optical imaging with various electrode-based techniques, we have designed and produced a skull-mounting "sliding-top cranial window" and a removable "electrode positioner microdrive". These new devices were used to study sensory processing in chronic and acute experiments in the cerebral cortices of cats and monkeys. This assembly allows simultaneous optical imaging of intrinsic signals or voltage-sensitive dyes combined with extracellular recording (single and multiple unit recording and local field potential), intracellular recording, microstimulation, or targeted injection of tracers. After the functional architecture is determined by optical imaging, electrodes are targeted into a selected cortical site under full visual control, at a variety of penetration angles (30-90 degrees ), accessing a large cortical area. The device consists of three parts: (1) a skull-mounting chamber, (2) a sliding cap, and (3) a microdrive. The microdrive can easily be removed and the cranial window is then sealed and covered with a flat protective cover. For chronic experiments, this arrangement allows the animal to be handled over a long period while fitted with a sealed cranial window of minimal volume and weight, and with negligible risk of accidental damage or infection.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Arieli A,Grinvald Adoi
10.1016/s0165-0270(02)00022-5subject
Has Abstractpub_date
2002-04-30 00:00:00pages
15-28issue
1eissn
0165-0270issn
1872-678Xpii
S0165027002000225journal_volume
116pub_type
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