Abstract：
:The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for unbiased estimation of anisotropic surfaces. The examples include descriptions of estimators of neurone and synapse number and sizes, synapse gradients, neurone point patterns in three-dimensional space, capillary surface area, and perikarya volumes. A major advantage of the methods is the possibility to carry out the estimation procedures in specified, well-characterized regions or layers of the brain. Some general statistical and stereological problems are briefly discussed.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Braendgaard H,Gundersen HJdoi
10.1016/0165-0270(86)90112-3subject
Has Abstractpub_date
1986-10-01 00:00:00pages
39-78issue
1-2eissn
0165-0270issn
1872-678Xpii
0165-0270(86)90112-3journal_volume
18pub_type
杂志文章abstract：:We used 24 sacral dorsal roots of the rat to analyze amplitude and area changes of biphasic and monophasic compound action potentials (CAPs) at 4 conduction distances. Both the CAPs of the A-fiber and C-fiber were analyzed. The changes were examined with the paired t test and linear regression. All the variables decre...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90064-x
更新日期：1993-03-01 00:00:00
abstract：:Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00072-6
更新日期：1996-08-01 00:00:00
abstract：INTRODUCTION:Our aim was to assess differences in movement measures in attention-deficit/hyperactivity disorder (ADHD) vs. typically developing (TD) controls. METHODS:We performed meta-analyses of published studies on motion measures contrasting ADHD with controls. We also conducted a case-control study with children/...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,meta分析
doi：10.1016/j.jneumeth.2015.03.001
更新日期：2015-08-30 00:00:00
abstract：:The present study describes the use of nose-poke habituation as a memory task and demonstrates that it is sensitive to hypo- and hypermnestic pharmacological treatments administered post-trial. Habituation of nose-poke behavior of rats was defined as a reduction in number of nose-pokes compared to baseline. It was mea...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00143-5
更新日期：1995-03-01 00:00:00
abstract：BACKGROUND:Tinnitus, head and ear noise, is due to maladaptive plastic changes in auditory and associated neural networks. Tinnitus has been traditionally managed through the use of sound to passively mask or facilitate habituation to tinnitus, a process that may take 6-12 months. NEW METHOD:A game-based perceptual tr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.04.002
更新日期：2015-07-15 00:00:00
abstract：:The terminal branches of motoneurons can be excited by electrical current pulses delivered via needles inserted intramuscularly. Observations are reported which indicate that tungsten semi-microelectrodes suitably positioned in a muscle belly can excite antidromically a large proportion of the alpha-motoneurons of tha...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90053-5
更新日期：1983-05-01 00:00:00
abstract：:A simple and easily constructed unit was designed using a buffer amplifier, a programmable transconductance amplifier and an active filter. The unit allows extracellular recording and stimulation through the same electrode. Thus in experiments where it is required to stimulate a nerve and record the effects of the sti...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90034-7
更新日期：1984-08-01 00:00:00
abstract：:We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.026
更新日期：2009-10-15 00:00:00
abstract：:Details are described of techniques which allow the isolation of labelled, identified neurones suitable for patch clamp recording from the guinea pig spinal cord. Fluorescent labels, injected into either the hindlimb muscles or the cerebellum, are retrogradely transported to motoneurones or dorsal spinocerebellar trac...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90037-9
更新日期：1987-05-01 00:00:00
abstract：:We have developed a novel double-labeling method to investigate multiple gene expression in single cells. The method relies on the use of a radioactive probe followed by a colorimetric probe. Unique to this method, the radioactive signal is first captured on an emulsion pre-coated slide, which totally separates it fro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00163-8
更新日期：1996-06-01 00:00:00
abstract：:A method is described for achieving gaze control in cats, by rewarding them for pushing against a lever with their tongue, only when a light-emitting diode (LED) was flickering. Since the state of the LED was changed sequentially from OFF to ON to flickering and the duration of flickering was short, the cats were requ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90036-9
更新日期：1980-10-01 00:00:00
abstract：BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108903
更新日期：2020-11-01 00:00:00
abstract：:Digital image analysis of quantitative autoradiographic (QAR) films is widely used in neuroscience applications. Unless proper precautions are taken when autoradiographic images are converted to digital form they can be inadvertently modified by improper application of the sampling process. This type of modification i...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90156-t
更新日期：1991-06-01 00:00:00
abstract：:The properties of 4 types of carbon electrodes designed for use as in vivo sensors of easily oxidized species in the mammalian brain have been evaluated in aqueous solutions at physiological pH. The electrodes are formed from a graphite-epoxy mixture, carbon paste, or carbon fibers, and have the geometries of a disk o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90058-x
更新日期：1984-03-01 00:00:00
abstract：:According to a basic formulation of the quantal model, evoked synaptic currents are made up of a linear summation of uniquantal synaptic currents, which in turn are equivalent to the spontaneous miniature synaptic currents ('minis') that often persist when evoked neurotransmitter release is blocked. Here I describe a ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.09.018
更新日期：2003-12-15 00:00:00
abstract：:A video-based 2D eye-tracking system (EyeLink version 2.04, SR Research Ltd/SMI) was compared with the scleral search coil technique for its performance on recording the properties of fixations and saccadic eye movements. Fixation positions and saccadic properties (amplitude, duration, and peak velocity) were calculat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00527-1
更新日期：2002-03-15 00:00:00
abstract：:Although dopamine (DA) translocation by the DA transporter (DAT) requires Na+, a role for Na+ in the DA recognition step in the translocation cycle has been questioned. Thus, when binding techniques were used to indirectly measure the affinity of DA for DAT via its potency in inhibiting cocaine analog binding, no stim...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00230-9
更新日期：2003-12-30 00:00:00
abstract：BACKGROUND:Demyelination is the end product of numerous pathological processes, and also is one of the main causes of neurological disability in Multiple sclerosis (MS). Research into the pathogenesis of MS is hampered by the conventional rodent models' inability to produce stable demyelination. NEW METHOD:Focal demye...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.05.024
更新日期：2018-09-01 00:00:00
abstract：:This paper presents a technique for stimulating and recording from multiple intact spinal roots in the in vitro mudpuppy (Necturus maculatus) spinal cord-forearm preparation using fine wire electrodes, a modified intrafascicular electrode. We found that multiple spinal roots of the preparation could be implanted with ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00176-4
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Image segmentation is often imperfect, particularly in complex image sets such z-stack micrographs of slice cultures and there is a need for sufficient details of parameters used in quantitative image analysis to allow independent repeatability and appraisal. NEW METHOD:For the first time, we have criticall...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.12.002
更新日期：2018-02-01 00:00:00
abstract：:Foraging is a rapid, side-to-side movement of the nose generated by Caenorhabditis elegans as it explores its environment. In this paper, we present an automated method to detect and analyze foraging behavior of C. elegans in a video sequence. Several morphological image-processing methods are used to locate the preci...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.027
更新日期：2008-06-15 00:00:00
abstract：:Bone morphogenetic protein 7 (BMP7), a member of the transforming growth factor β (TGF-β) superfamily, plays important roles in the development of various tissues and organs in mouse and human. In particular, BMP7 is critical for the formation of the nervous system and it is considered to have therapeutic potential in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.12.007
更新日期：2012-03-30 00:00:00
abstract：:Computer-based sleep scoring systems are often calibrated by reference to a conventional visual analysis of electroencephalographic (EEG) and electromyographic (EMG) traces. However, these types of data place high demands on digital storage capacity which may limit the duration or feasibility of some studies. The pres...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.04.004
更新日期：2005-10-15 00:00:00
abstract：BACKGROUND:Current assessment of sport-related concussion involves a multifaceted approach including neurocognitive testing, symptom report, and balance assessment. Lacking from this paradigm is a truly objective measure of the physiological changes associated with concussion. NEW METHOD:A device called the Brain Acou...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.003
更新日期：2014-01-15 00:00:00
abstract：:The coupling of Ca(2+) influx to synaptic vesicle (SV) recycling in nerve terminals is essential for neurotransmitter release and thus neuronal communication. Both of these parameters have been monitored using fluorescent reporter dyes such as fura-2 and FM1-43 in single central nerve terminals. However, their simulta...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.09.012
更新日期：2007-03-15 00:00:00
abstract：:An encephalomyosynangiosis (EMS) is a temporal muscle graft that is placed onto the surface of the brain to serve as a source for collateral vessel growth for brain revascularization in patients with Moyamoya Disease (MMD). To facilitate an EMS in patients with occlusive cerebrovascular diseases other than MMD, the tr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.07.011
更新日期：2011-09-30 00:00:00
abstract：:In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90179-3
更新日期：1988-03-01 00:00:00
abstract：:Since the advent of optogenetics, the technology development has focused on new methods to optically interact with single nerve cells. This gave rise to the field of photonic neural interfaces, intended as the set of technologies that can modify light radiation in either a linear or non-linear fashion to control and/o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2019.108355
更新日期：2019-09-01 00:00:00
abstract：BACKGROUND:Neural coding of sound information is often studied through frequency tuning curve (FTC), spectro-temporal receptive field (STRF), post-stimulus time histogram (PSTH), and other methods such as rate functions. These methods, despite providing a robust characterization of auditory responses in their specific ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108682
更新日期：2020-05-15 00:00:00
abstract：:The neocortex is the site of origin of several forms of acquired epilepsy. Here we provide a brief review of experimental models that were recently developed to study neocortical epileptogenesis as well as some major results obtained with these methods. Most of neocortical seizures appear to be nocturnal and it is kno...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.08.030
更新日期：2016-02-15 00:00:00
