Abstract:
:The properties of 4 types of carbon electrodes designed for use as in vivo sensors of easily oxidized species in the mammalian brain have been evaluated in aqueous solutions at physiological pH. The electrodes are formed from a graphite-epoxy mixture, carbon paste, or carbon fibers, and have the geometries of a disk or a cylinder. The voltammetric properties of several catecholamines, some of their metabolites and precursors, uric acid, and ascorbic acid are reported at unmodified carbon surfaces. The problem of overlap of the voltammetric waves of ascorbate and catechols is addressed, and two different methods which minimize this problem are examined. These are the use of disk-shaped electrodes fabricated from carbon fibers, which facilitate the use of subtracted voltammograms to determine small changes in the concentration of catecholamines in the presence of ascorbic acid, and the use of electrochemically modified, cylindrically-shaped electrodes also fabricated from carbon fibers. Voltammetry at the modified electrodes gives evidence the catechols can be resolved from ascorbate, and that catechols, but not ascorbic acid, adsorb to the electrode surface.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kovach PM,Ewing AG,Wilson RL,Wightman RMdoi
10.1016/0165-0270(84)90058-xsubject
Has Abstractpub_date
1984-03-01 00:00:00pages
215-27issue
3eissn
0165-0270issn
1872-678Xpii
0165-0270(84)90058-Xjournal_volume
10pub_type
杂志文章abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease. LRRK2 kinase activity is required for toxicity in neuronal cell cultures suggesting that selective kinase inhibitors may prevent neurodegeneration in patients. Directly monitoring LRRK2 activity in cells would b...
journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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