Abstract：
:Chronic spinal catheterization via an atlanto-occipital puncture (CAO) has been widely used to study the effects of drugs on spinal nociceptive mechanisms, but this method is associated with spinal cord damage that may change the efficacy of spinally injected analgesics. Using a slight modification of the method of Storkson et al. (J. Neurosci. Methods 65 (1996) 167), the rat spinal cord was acutely catheterized via a lumbar puncture (AL) and the potency of morphine-induced antinociception in the tail flick test was comparatively studied in animals with or without a CAO catheter. The opiate potency via an AL catheter (AD50; 95% confidence limits) was significantly more intense in rats without (0.29 microg; 0.19-0.47) than in rats with a CAO catheter (1.1 microg; 0.87-1.47) and stronger than via a CAO catheter (8.2 microg; 4.6-14.4). The potency of morphine via a CAO catheter was significantly improved in indomethacin-pretreated rats (1 mg/kg, i.p., twice a day for 5 days), thus indicating that inflammatory changes produced by a CAO catheter are at least in part the reason for the lower efficacy of the opiate. The use of an AL catheter minimizes such spinal changes and permits acute experimental protocols in which more than one spinal injection is necessary.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Prado WAdoi
10.1016/s0165-0270(03)00197-3subject
Has Abstractpub_date
2003-10-15 00:00:00pages
33-9issue
1eissn
0165-0270issn
1872-678Xpii
S0165027003001973journal_volume
129pub_type
杂志文章abstract：:In many experimental designs, animal observation is associated with local field potential (LFP) recordings in order to find correlations between behavior dynamics and neuronal activity. In such cases, relevant behaviors can occur at different times during free-running recordings and should be put together by the time ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.08.027
更新日期：2008-01-30 00:00:00
abstract：BACKGROUND:The assessment of directed functional connectivity from neuronal data is increasingly common in neuroscience by applying measures based in the Granger causality (GC) framework. Although initially these consisted in simple analyses based on directionality strengths, current methods aim to discriminate causal ...
journal_title：Journal of neuroscience methods
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更新日期：2015-04-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90048-i
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90023-k
更新日期：1993-04-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.07.014
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.09.005
更新日期：2011-01-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.11.031
更新日期：2012-03-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00015-8
更新日期：2002-04-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90098-k
更新日期：1991-10-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.033
更新日期：2012-07-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90070-7
更新日期：1987-04-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.06.011
更新日期：2012-07-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.01.009
更新日期：2006-09-15 00:00:00
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2013.10.009
更新日期：2014-01-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90141-x
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90071-k
更新日期：1992-12-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00341-2
更新日期：2003-02-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.02.023
更新日期：2011-04-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.04.007
更新日期：2010-06-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(92)90061-h
更新日期：1992-06-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.018
更新日期：2018-04-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00401-0
更新日期：2001-08-15 00:00:00
abstract：:Event-related potentials (ERPs) are evoked brain potentials that are averaged across many trial repetitions with individual trials aligned (i.e. time-locked) to a specific behavioral event, typically the onset of the stimulus (s-lock) or the onset of the behavioral response (r-lock). These evoked potential averages ma...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00194-5
更新日期：1998-03-13 00:00:00
abstract：:Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90092-6
更新日期：1987-11-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.06.026
更新日期：2018-09-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.07.003
更新日期：2005-02-15 00:00:00
