Abstract:
:Practical aspects on the use of horseradish peroxidase (HRP) and wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) to trace peripheral cutaneous nerve endings have been studied. The parameters studied included application site of the tracer, post-application survival time, tracer concentration and tracer volume. These parameters were examined in the glabrous skin of the rat hindpaw. The best results were obtained with injections of 1 microliter WGA-HRP (20 micrograms/microliter) in dorsal root ganglia innervating the examined cutaneous region and a postinjection survival time of 18-36 h. With this approach extensive and heavy labeling was achieved of epidermal nerve endings, nerve endings in Merkel cell-neurite complexes and Meissner corpuscles. Useful, but less extensive labeling of these types of peripheral nerve endings, was obtained with injections of HRP in the lumbar spinal cord dorsal horn.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Aldskogius H,Kinnman E,Persson Jdoi
10.1016/0165-0270(86)90141-xsubject
Has Abstractpub_date
1986-02-01 00:00:00pages
281-94issue
4eissn
0165-0270issn
1872-678Xpii
0165-0270(86)90141-Xjournal_volume
15pub_type
杂志文章abstract:BACKGROUND:Current in vivo methods cannot distinguish between the roles of vascular and stationary tissular gas bubbles in the mechanisms of decompression sickness (DCS). NEW METHOD:To answer this question, we designed a normobaric-hyperbaric chamber for studying specifically the contribution of stationary tissular ga...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.07.010
更新日期:2014-10-30 00:00:00
abstract::Stress has long been known to affect grooming in rodent species, altering both its activity measures and behavioural microstructure. Since stress disturbs a general pattern of self-grooming uninterrupted cephalocaudal progression, the grooming analysis algorithm (Kalueff and Tuohimaa, Brain Res. Protocols, 2004; 15: 1...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.10.001
更新日期:2005-04-30 00:00:00
abstract::Since the publication by Marmarou of a new rodent model of head injury, an increasing number of centres are adopting the model for use in a variety of paradigms. We report on two simple methods we have applied which allows us to monitor weight drop velocity and foam stiffness, both of which are critical factors for pr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00046-5
更新日期:1996-11-01 00:00:00
abstract::According to a basic formulation of the quantal model, evoked synaptic currents are made up of a linear summation of uniquantal synaptic currents, which in turn are equivalent to the spontaneous miniature synaptic currents ('minis') that often persist when evoked neurotransmitter release is blocked. Here I describe a ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.09.018
更新日期:2003-12-15 00:00:00
abstract:BACKGROUND:Polarized light imaging (PLI) has evolved into a powerful neuroimaging tool to analyze fiber tracts with submillimeter resolution in microtome sections of postmortem human brain tissue. In PLI polarized light changes its polarization state while passing through birefringent tissue, i.e., myelinated axons, wh...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.08.022
更新日期:2013-10-30 00:00:00
abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2006.04.009
更新日期:2006-07-15 00:00:00
abstract::Over the past few years there has been an increased interest in studying the underlying neural mechanism of attention and cognitive brain activity. This paper aims towards identifying and analyzing distinct responses in an auditory working memory paradigm, as independent components with variable latency, frequency and...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.02.001
更新日期:2011-04-30 00:00:00
abstract::Implantable neural micro-electrode arrays have the potential to restore lost sensory or motor function to many different areas of the body. However, the invasiveness of these implants often results in scar tissue formation, which can have detrimental effects on recorded signal quality and longevity. Traditional histol...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.06.001
更新日期:2013-08-15 00:00:00
abstract:BACKGROUND:Cerebral air emboli occur as a complication of invasive medical procedures. The sensitivity of cerebral monitoring methods for the detection of air emboli is not known. This study investigates the utility of electroencephalography and non-invasively measured cerebral oxygen saturation in the detection of int...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.03.009
更新日期:2014-05-15 00:00:00
abstract::Microglia are brain cells of considerable interest because of their role in CNS inflammatory responses and strong association with neuritic plaques in Alzheimer's disease (AD). In the present study, immunocytochemistry was combined with unbiased stereology to estimate the mean total number of microglia in dentate gyru...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00100-9
更新日期:1998-10-01 00:00:00
abstract:BACKGROUND:Experimental investigation of sleep-wake dynamics in animals is an important part of pharmaceutical development. Typically, it involves recording of electroencephalogram, electromyogram, locomotor activity, and electrooculogram. Visual identification, or scoring, of the sleep-wake states from these recording...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108668
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:Virtual reality-based neuropsychological assessments proffer the potential to address the limited ecological validity of pen-and-paper measures of memory. NEW METHOD:To investigate the construct validity of a newly developed virtual reality-based multiple errands task, the Virtual Environment Grocery Store ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.07.027
更新日期:2017-11-01 00:00:00
abstract::We developed a system for quantitatively measuring arm movement. Our approach provides a method to simultaneously capture upper limb kinetic and kinematic data during assisted passive arm movements. Data are analysed with respect to Cartesian and upper limb coordinate systems to obtain upper limb joint angles and torq...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.03.004
更新日期:2011-04-30 00:00:00
abstract::We characterised the dynamics in the oscillatory potentials (OPs) of the rat electroretinogram (ERG) using a continuous complex Morlet wavelet transform. Dark-adapted (>12h) full field ERG responses were recorded from five anaesthetized (ketamine:xylazine, 60:5mg/kg) adult Long-Evans rats (10-12weeks). Five responses ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.12.007
更新日期:2008-03-30 00:00:00
abstract::This paper describes a feedback system in which a neutral density wedge automatically controls the light flux applied to a photoreceptor, so that an intracellular recorded response is held constant. We describe here a "differential sampler" that subtracts the photoreceptors dark response from the light response induce...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90093-6
更新日期:1983-08-01 00:00:00
abstract:BACKGROUND:Negative emotional states resulting from concussion are of increasing concern. In the current study, we developed a model to investigate negative affect following concussion in the projectile concussive impact (PCI) model. High frequency ultrasonic vocalizations (22kHz USVs) are associated with negative affe...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.10.017
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:The classification of sleep signals is a subjective and time consuming task. A large number of automatic classifiers have been published in the past decade but a sleep community has no strong confidence to use them in clinical practice and still remains using a standard manual scoring according standardized ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.01.013
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Despite extensive environmental standardization and the use of genetically and microbiologically defined mice of similar age and sex, individuals of the same mouse inbred strain commonly differ in quantitative traits. This is a major issue as it affects the quality of experimental results. Standard analysis ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108810
更新日期:2020-09-01 00:00:00
abstract::The detection and characterization of bursting activity remains a topic where no consensual definition has been reached so far. We compare here three different approaches of spike trains variability: statistical characterization (average frequency, coefficient of variation), burst detection (Poisson and rank surprise)...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.01.020
更新日期:2009-04-30 00:00:00
abstract:BACKGROUND:Flow cytometry is an efficient and powerful technique to characterize and quantify numerous cells. However, the strengths of this technique have not been widely harnessed in neurosciences due to the critical step of CNS tissue preparation into a single cell suspension. Previous reports assessed either neural...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.03.021
更新日期:2015-05-30 00:00:00
abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease. LRRK2 kinase activity is required for toxicity in neuronal cell cultures suggesting that selective kinase inhibitors may prevent neurodegeneration in patients. Directly monitoring LRRK2 activity in cells would b...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.12.015
更新日期:2013-03-30 00:00:00
abstract:BACKGROUND:To map and quantify the proton exchange rate (kex) of brain tissues using improved omega plots in ischemic stroke patients and to investigate whether kex can serve as a potential endogenous surrogate imaging biomarker for detecting the metabolic state and the pathologic changes due to ischemic stroke. NEW M...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108926
更新日期:2020-12-01 00:00:00
abstract::A Lucite plate reconstruction method is described which, when combined with HRP histochemistry, provides an excellent means of visualizing, in 3-dimensional fashion, the functional organization of neuronal populations. Color photomicrographs of representative serial sections were made through the baboon oculomotor nuc...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(81)90019-4
更新日期:1981-06-01 00:00:00
abstract:BACKGROUND:Whole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between a glass pipette electro...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.109008
更新日期:2021-01-15 00:00:00
abstract::A method for automatically analyzing cortical signals recorded with voltage-sensitive dyes and a photodiode array is described. First, a forward-backward non-linear filtering technique is used to eliminate the background noise and preserve the fast transients of the signals. Then the filtered signals are deconvoluted ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00105-0
更新日期:1995-12-01 00:00:00
abstract::Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to prese...
journal_title:Journal of neuroscience methods
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.jneumeth.2013.07.016
更新日期:2013-10-15 00:00:00
abstract::We describe a method for estimating the average proportion of GABAergic terminal area relative to total nerve terminal area with confocal microscopy. Nerve terminal regions were identified with dual colour immunofluorescence on Vibratome sections with an antibody to synaptophysin (SYN), and GABAergic processes, includ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00163-6
更新日期:2000-01-31 00:00:00
abstract::Detecting brain structural changes from magnetic resonance (MR) images can facilitate early diagnosis and treatment of neurological and psychiatric diseases. Many existing methods require an accurate deformation registration, which is difficult to achieve and therefore prevents them from obtaining high accuracy. We de...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.09.001
更新日期:2014-01-15 00:00:00
abstract:BACKGROUND:Functional magnetic resonance imaging (fMRI) is commonly used to infer hemodynamic changes in the brain after increased neural activity, measuring the blood oxygen level-dependent (BOLD) signal. An important challenge in the analyses of fMRI data is to develop methods that can accurately deconvolve the BOLD ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.07.009
更新日期:2018-10-01 00:00:00
abstract::Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(92)90061-h
更新日期:1992-06-01 00:00:00