Abstract:
:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and object parameters in the nervous system. The following areas of methodology have been shown to be important: (1) use of buffers free of metallic ions for tissue processing, (2) selection and titration of first and second layer antibodies, (3) reduction and control of fading of fluorescence, (4) selection of microscopical and imaging equipment to give accurate, sensitive and uniform representations of low-light biological images, and (5) use of appropriate image analysis algorithms in order to generate binary images that match the spatial and intensity distributions of immunostaining. Incorporation of these techniques into our assay system gave sensitive measurements of the time-scale of uptake of 5-hydroxy-tryptamine (5-HT) into sympathetic nerve terminals. The microscopical assay appears to have advantages over alternative approaches used for studies of neurotransmitter dynamics, particularly in small, heterogeneous tissue samples.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Cowen T,Thrasivoulou Cdoi
10.1016/0165-0270(92)90048-isubject
Has Abstractpub_date
1992-10-01 00:00:00pages
107-16issue
1-2eissn
0165-0270issn
1872-678Xpii
0165-0270(92)90048-Ijournal_volume
45pub_type
杂志文章abstract::The electrochemical technique of differential pulse voltammetry (DPV) with micro-biosensors has been used for a number of years to monitor in vivo and in situ changes in the extracellular concentration of cerebral ascorbic acid, as well as that of the metabolites of dopamine (DA) and serotonin (5-HT). We have recently...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(92)90094-t
更新日期:1992-05-01 00:00:00
abstract::Principal components analysis (PCA) was used to define the linearly dependent factors underlying sensory information processing in the vibrissal sensory area of the ventral posterior medial (VPM) thalamus in eight awake rats. Ensembles of up to 23 single neurons were simultaneously recorded in this area, either during...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00130-2
更新日期:1999-12-15 00:00:00
abstract::Capsule application of Diamidino Yellow (DY) to the cut end of the sciatic nerve immediately followed by capsule application of Fast Blue (FB) resulted in approximately 95% double-labelled dorsal root ganglion neurones (DRGn) and motoneurones (Mn). Nerve injection of DY followed either immediately or 2 months later by...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00164-8
更新日期:2000-02-15 00:00:00
abstract:BACKGROUND:Recent research has shown that there is a strong correlation between the functional properties of a neuron and its morphologic structure. Current morphologic analyses typically involve a significant component of computer-assisted manual labor, which is very time-consuming and is susceptible to operator bias....
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.05.020
更新日期:2007-09-15 00:00:00
abstract::We present a client-server application for the distributed multivariate analysis of time series using standard PCs. We here concentrate on analyses of multichannel EEG/MEG data, but our method can easily be adapted to other time series. Due to the rapid development of new analysis techniques, the focus in the design o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.002
更新日期:2006-04-15 00:00:00
abstract::According to a basic formulation of the quantal model, evoked synaptic currents are made up of a linear summation of uniquantal synaptic currents, which in turn are equivalent to the spontaneous miniature synaptic currents ('minis') that often persist when evoked neurotransmitter release is blocked. Here I describe a ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.09.018
更新日期:2003-12-15 00:00:00
abstract::Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00352-6
更新日期:2001-04-30 00:00:00
abstract::The whole cell patch clamp technique allows recording of membrane currents in an entire cell under voltage clamp conditions. However, technical difficulties arise in large cells bearing extensive processes, such as human fetal dorsal root ganglion (DRG) neurons in culture. In order to improve space clamp conditions, h...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90094-v
更新日期:1990-10-01 00:00:00
abstract::Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. W...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)02237-1
更新日期:1997-05-16 00:00:00
abstract:BACKGROUND:The detection of high-frequency oscillations (HFOs, ripples: 80-200 Hz, fast ripples: 250-500 Hz) is often based on considering ripples or fast ripples in isolation; overlapping ripples and fast ripples are excluded from further analysis. Here, we studied overlapping HFOs during spontaneous seizures in piloc...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.02.011
更新日期:2018-04-01 00:00:00
abstract::A Guard-PAK precolumn system was used for the reversed-phase liquid chromatography purification of a small, synthetic radiolabeled opioid peptide, FK 33-824 (D-Ala2, methyl-phe4, Met (O)ol5 enkephalin) (FK). This procedure involves trace enrichment of iodinated peptide onto the precolumn while iodination reagents are ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(92)90052-f
更新日期:1992-02-01 00:00:00
abstract::There are 3 known forms of synaptic plasticity at CNS synapses: long-term potentiation (LTP) mediated by NMDA receptor activation, LTP mediated by voltage-dependent calcium channel (VDCC) activation, and long-term depression (LTD) mediated by the NMDA receptor. All 3 forms of synaptic plasticity can be observed in hip...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00188-m
更新日期:1995-06-01 00:00:00
abstract::The ability to detect and sort overlapping spike waveforms in extracellular recordings is key to studies of neural coding at high spatial and temporal resolution. Most spike-sorting algorithms are based on initial spike detection (e.g. by a voltage threshold) and subsequent waveform classification. Much effort has bee...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.011
更新日期:2008-09-15 00:00:00
abstract::Amyloid-beta (Abeta) deposits are one of the hallmarks of the neuropathological degeneration observed in Alzheimer's disease (AD) and Abeta concentrations have been reported to vary in different brain regions of AD patients. Abeta is produced by the sequential cleavage of amyloid precursor protein (APP) by beta-secret...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.05.001
更新日期:2006-10-30 00:00:00
abstract::Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(92)90061-h
更新日期:1992-06-01 00:00:00
abstract::Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultur...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00025-8
更新日期:1996-10-21 00:00:00
abstract::A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00207-w
更新日期:1995-07-01 00:00:00
abstract:BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108634
更新日期:2020-04-15 00:00:00
abstract::Voltage-sensitive dyes (VSDs) and optical imaging are useful for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes we were able to detect neuronal activity in single trials before it could be detected by local field potential (LFP) recordi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00511-8
更新日期:2002-03-30 00:00:00
abstract:BACKGROUND:Glucose hypermetabolism in denervated skeletal muscle suggests the potential for developing a diagnostic tool for peripheral nerve injuries. Herein, we investigated the characteristics and molecular mechanism of this phenomenon. NEW METHOD:Temporal course of glucose hypermetabolism and development of abnorm...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.01.014
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:The local field potential (LFP) is usually calculated from current sources arising from transmembrane currents, in particular in asymmetric cellular morphologies such as pyramidal neurons. NEW METHOD:Here, we adopt a different point of view and relate the spiking of neurons to the LFP through efferent synap...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108871
更新日期:2020-10-01 00:00:00
abstract::Knowledge of the precise position of recording microelectrodes within the brain of a non-human primate is essential for a reliable exploration of very small anatomic structures. This work demonstrates the compatibility of a newly developed glass-guided microelectrode design and microfeed equipment with high-resolution...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.10.018
更新日期:2006-06-15 00:00:00
abstract::We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.06.026
更新日期:2009-10-15 00:00:00
abstract::Intrathecal (IT) administration is an important route of drug delivery, and its modelling in a large animal species is of critical value. Although domestic swine is the preferred species for preclinical pharmacology, no minimally invasive method has been established to deliver agents into the IT space. While a "blind"...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.03.006
更新日期:2013-05-30 00:00:00
abstract::A discontinuous gradient of Percoll has been used to prepare a subcellular fraction (fraction III) from fish brain which is greatly enriched in synaptic nerve endings (synaptosomes). Synaptosomes display well-preserved morphology, good functional performance as assessed by ability to release preloaded neurotransmitter...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(96)00137-9
更新日期:1997-02-01 00:00:00
abstract::Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90114-8
更新日期:1988-08-01 00:00:00
abstract::The incidence of leptomeningeal carcinomatosis is on the rise and current treatment modalities have limited efficacy. The development of new treatment strategies has been hampered by the lack of an appropriate animal model that accurately parallels the clinical condition. We have developed a new surgical technique for...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00115-0
更新日期:1998-11-01 00:00:00
abstract::A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90062-8
更新日期:1987-08-01 00:00:00
abstract::An important factor in reducing variability in mouse test outcomes has been to develop assays that can be used for continuous automated home cage assessment. Our experience has shown that this has been most evidenced in long-term assessment of wheel-running activity in mice. Historically, wheel-running in mice and oth...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.04.014
更新日期:2018-04-15 00:00:00
abstract::The detection of epileptiform discharges in the EEG is an important component in the diagnosis of epilepsy. In this study, multiple signal classification (MUSIC), autoregressive (AR) and periodogram methods were used to get power spectra in patients with absence seizure. The EEG power spectra were used as an input to ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.04.009
更新日期:2005-10-30 00:00:00