Abstract：
:Intrathecal (IT) administration is an important route of drug delivery, and its modelling in a large animal species is of critical value. Although domestic swine is the preferred species for preclinical pharmacology, no minimally invasive method has been established to deliver agents into the IT space. While a "blind" lumbar puncture (LP) can sample cerebrospinal fluid (CSF), it is unreliable for drug delivery in pigs. Using computed tomography (CT), we determined the underlying anatomical reasons for this irregularity. The pig spinal cord was visualised terminating at the S2-S3 level. The lumbar region contained only small amounts of CSF found in the lateral recess. Additional anatomical constraints included ossification of the midline ligaments, overlapping lamina with small interlaminar spaces, and a large bulk of epidural adipose tissue. Accommodating the the pig CT anatomy, we developed a lateral LP (LLP) injection technique that employs advanced planning of the needle path and monitoring of the IT injection progress. The key features of the LLP procedure involved choosing a vertebral level without overlapping lamina or spinal ligament ossification, a needle trajectory crossing the midline, and entering the IT space in its lateral recess. Effective IT delivery was validated by the injection of contrast media to obtain a CT myelogram. LLP represents a safe and reliable method to deliver agents to the lumbar pig IT space, which can be implemented in a straightforward way by any laboratory with access to CT equipment. Therefore, LLP is an attractive large animal model for preclinical studies of IT therapies.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Pleticha J,Maus TP,Jeng-Singh C,Marsh MP,Al-Saiegh F,Christner JA,Lee KH,Beutler ASdoi
10.1016/j.jneumeth.2013.03.006subject
Has Abstractpub_date
2013-05-30 00:00:00pages
10-5issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(13)00111-8journal_volume
216pub_type
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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