Abstract:
:Immunohistochemistry using antiserum against the basement membrane glycoprotein laminin, was shown to be an excellent marker for brain blood vessels. Throughout the brain of mice, rats, guinea pigs, monkeys and humans, the basement membrane of the vascular structures were strongly laminin-positive. The neuropil itself was laminin-negative, whereas a positive reaction was observed in the meaninges. When the laminin antiserum was preabsorbed with its proper antigen, no specific fluorescence was observed. Using India ink perfusion as a comparative method, it was found that probably all vascular structures were also visualized with laminin immunohistochemistry. Laminin immunofluorescence was found well-suited for computer-assisted quantitative image analysis of brain vascularity. As expected in the periphery, the basement membrane of many other structures except blood vessels such as endoneurium, epithelium and smooth muscle cells were laminin-positive. Although the vascular network was also strongly laminin-immunoreactive, it was difficult to differentiate between blood vessels and non-vascular structures in the periphery as compared to the central nervous system. In conclusion, laminin immunohistochemistry has proven to be a simple, useful and specific method to study vascular structures in the central nervous system and an excellent alternative to more conventional and laborious methods such as perfusion with India ink.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Eriksdotter-Nilsson M,Björklund H,Olson Ldoi
10.1016/0165-0270(86)90128-7subject
Has Abstractpub_date
1986-09-01 00:00:00pages
275-86issue
4eissn
0165-0270issn
1872-678Xpii
0165-0270(86)90128-7journal_volume
17pub_type
杂志文章abstract::Facial expressions of emotions are important in nonverbal communication. Although numerous neural structures have been identified to be involved in emotional face processing, the amygdala is thought to be a core moderator. While previous studies have relied on facial images of humans, the present study is concerned wi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.10.016
更新日期:2007-03-30 00:00:00
abstract::Imaging technologies, such as intrinsic optical imaging (IOI), functional magnetic resonance imaging (fMRI) or multiphoton microscopy provide excellent opportunities to study the relationship between functional signals recorded from a cortical area and the underlying anatomical structure. This, in turn, requires accur...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.07.029
更新日期:2011-09-30 00:00:00
abstract::The physical basis for electrical stimulation of excitable tissue, as used by electrophysiological researchers and clinicians in functional electrical stimulation, is presented with emphasis on the fundamental mechanisms of charge injection at the electrode/tissue interface. Faradaic and non-Faradaic charge transfer m...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2004.10.020
更新日期:2005-02-15 00:00:00
abstract::Electrical potentials recorded extracellularly from the sinus gland of the isopod, Oniscus asellus, were stored on video tape with the aid of a digital-audio (DA) processor and a video cassette recorder (VCR). The DA processor transforms the analog signal to digital pulses of equal amplitude and converts these pulses ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90058-5
更新日期:1985-10-01 00:00:00
abstract::The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.027
更新日期:2012-01-30 00:00:00
abstract::Larval locomotion is a sensitive readout of a range of nervous system deficits in Drosophila, and has been utilised to quantify modulation of the disease phenotype in models of human disease. Single larvae are typically analysed in series using manual quantification of parameters such as contraction rate, or grouped t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.08.037
更新日期:2012-01-30 00:00:00
abstract::Intrinsic signal imaging (ISI) measures changes in light reflectance from the illuminated cortex (intrinsic signals or IS) attributed to various vascular and metabolic sources that, when using illumination in the 600 nm range, appear to co-localize with neuronal activity. Given the multiple sources contributing to the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00180-1
更新日期:2000-04-15 00:00:00
abstract::Neuroendocrine cells like chromaffin cells and PC-12 cells are established models for transport, docking and secretion of secretory vesicles. In micrographs, these vesicles are recognized by their electron dense core. The analysis of secretory vesicle distribution is usually performed manually, which is labour-intensi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.05.022
更新日期:2008-08-15 00:00:00
abstract::Prepulse inhibition (PPI) of the acoustic startle response is a measure of sensory motor gating, and is affected in various neuropsychiatric disorders. Although PPI has been used extensively to study both the neural effects of such conditions, as well as in the search for animal models, a number of critical issues hav...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.014
更新日期:2008-08-30 00:00:00
abstract:BACKGROUND:Deviant stimuli within a standard, frequent stimulus train induce a cortical somatosensory mismatch response (SMMR). The SMMR reflects the brain's automatic mechanism for the detection of change in a somatosensory domain. It is usually elicited by electrical stimulation, which activates nerve fibers and rece...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.09.014
更新日期:2019-01-01 00:00:00
abstract::The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00328-4
更新日期:2001-03-30 00:00:00
abstract::We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Foll...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(05)80009-3
更新日期:1993-06-01 00:00:00
abstract::The FD Rapid GolgiStain Kit (FD NeuroTechnologies, Inc., Ellicott City, MD) was developed using an improved Golgi-Cox impregnation method to provide stable, sensitive, and convenient staining of mature neurons. However, embryonic neurons cannot be clearly visualized using this method. We have further improved the Golg...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.06.007
更新日期:2012-07-30 00:00:00
abstract:BACKGROUND:In this paper exploitation of correlation between post-movement beta rebound in EEG and blood oxygenation level dependent (BOLD) in fMRI is addressed. Brain studies do not reveal any clear relationship between synchronous neuronal activity and BOLD signal. Simultaneous recording of EEG and fMRI provides a gr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.07.018
更新日期:2015-10-30 00:00:00
abstract::In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90179-3
更新日期:1988-03-01 00:00:00
abstract::Using the novel mathematical technique known as wavelet analysis, a new method (WSC) is presented to sort spikes according to a decomposition of neural signals in the time-frequency space. The WSC method is implemented by a pyramidal algorithm that acts upon neural signals as a bank of quadrature mirror filters. This ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00250-8
更新日期:2000-09-15 00:00:00
abstract::A reliable focal cerebral ischemia model is essential in evaluating the efficacy and safety of neuroprotective therapy for stroke. We present a focal embolic ischemic model of rat with reproducible and predictable brain infarction by comparing it with two other most commonly used focal cerebral ischemia models. To pro...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00142-5
更新日期:2002-08-30 00:00:00
abstract:BACKGROUND:The application of single-cell RNA sequencing (scRNASeq) represents a unique approach to identify hundreds to millions of cells in mammalian cortical multilayers at different stages of embryogenesis. ScRNASeq technology applied to neurological studies requires the use of fresh starting materials because stan...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108960
更新日期:2021-01-01 00:00:00
abstract::A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00061-5
更新日期:1995-11-01 00:00:00
abstract::We characterised the dynamics in the oscillatory potentials (OPs) of the rat electroretinogram (ERG) using a continuous complex Morlet wavelet transform. Dark-adapted (>12h) full field ERG responses were recorded from five anaesthetized (ketamine:xylazine, 60:5mg/kg) adult Long-Evans rats (10-12weeks). Five responses ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.12.007
更新日期:2008-03-30 00:00:00
abstract::Evaluation of electron microscopic images of serial sections is a time-consuming process requiring a high level of expertise. Here we present an algorithm to ease and accelerate this process. It is a modification of an algorithm successfully used in computer vision for object recognition. However, rather than recognis...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00439-3
更新日期:2001-10-30 00:00:00
abstract:BACKGROUND:Events that instigate disease may involve biochemical events distinct from changes in the steady-state levels of proteins. Even chronic degenerative disorders appear to involve changes such as post-translational modifications. NEW METHOD:We have begun a series of proteomics analyses on proteins that have be...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.11.005
更新日期:2019-05-01 00:00:00
abstract::Intercellular calcium waves in central nervous system astrocyte networks underline the principle mechanism of cell signaling in astrocyte syncsytiums, which putatively contribute to the modulation of neuronal signaling and metabolic regulation. In support of carrying out systems level analyses of astrocyte networks, w...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.01.013
更新日期:2008-05-30 00:00:00
abstract:BACKGROUND:Glucose hypermetabolism in denervated skeletal muscle suggests the potential for developing a diagnostic tool for peripheral nerve injuries. Herein, we investigated the characteristics and molecular mechanism of this phenomenon. NEW METHOD:Temporal course of glucose hypermetabolism and development of abnorm...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.01.014
更新日期:2019-04-01 00:00:00
abstract::The most direct evaluation of human brain activity has been obtained from intracranial electrodes placed either on the surface of the brain or inserted into the brain to record from deep brain structures. Currently, the placement of intracranial electrodes implies transcranial surgery, either through a burr hole or a ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.12.027
更新日期:2013-03-30 00:00:00
abstract::Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00057-0
更新日期:1998-08-01 00:00:00
abstract::The Utah Intracortical Electrode Array (UIEA) is an array of 100 penetrating silicon microelectrodes designed to focally electrically stimulate or record neurons residing in a single layer up to 1.5 mm beneath the surface of the cerebral cortex. Apart from its use as a unique tool to study parallel processing in the c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00031-4
更新日期:1998-07-01 00:00:00
abstract:BACKGROUND:This manuscript describes a new, multidimensional and data-driven approach to identify outlying datapoints from a first-level fMRI dataset. NEW METHOD:Using three different indicators of data corruption (the fast variance component of DVARS [Δ%D-var], scan-to-scan total displacement [STS], and each scan's o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.02.008
更新日期:2019-04-15 00:00:00
abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2006.04.009
更新日期:2006-07-15 00:00:00
abstract:BACKGROUND:Synchronous detecting neuron spikes and dopamine (DA) activities in the non-human primate brain play an important role in understanding of Parkinson's disease (PD). At present, most experiments are carried out by combing of electrodes and commercial instruments, which are inconvenient, time-consuming and ine...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.04.015
更新日期:2018-07-01 00:00:00