Abstract：
BACKGROUND:In order to evaluate nerve regeneration in clinically relevant hindlimb surgical paradigms not feasible in fluorescent mice models, we developed a rat that expresses green fluorescent protein (GFP) in neural tissue. METHODS:Transgenic Sprague-Dawley rat lines were created using pronuclear injection of a transgene expressing GFP under the control of the thy1 gene. Nerves were imaged under fluorescence microscopy and muscles were imaged with confocal microscopy to determine GFP expression following sciatic nerve crush, transection and direct suturing, and transection followed by repair with a nerve isograft from nonexpressing littermates. RESULTS:In each surgical paradigm, fluorescence microscopy demonstrated the loss and reappearance of fluorescence with regeneration of axons following injury. Nerve regeneration was confirmed with imaging of Wallerian degeneration followed by reinnervation of extensor digitorum longus (EDL) muscle motor endplates using confocal microscopy. CONCLUSION:The generation of a novel transgenic rat model expressing GFP in neural tissue allows in vivo imaging of nerve regeneration and visualization of motor endplate reinnervation. This rat provides a new model for studying peripheral nerve injury and regeneration over surgically relevant distances.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Moore AM,Borschel GH,Santosa KB,Flagg ER,Tong AY,Kasukurthi R,Newton P,Yan Y,Hunter DA,Johnson PJ,Mackinnon SEdoi
10.1016/j.jneumeth.2011.10.011subject
Has Abstractpub_date
2012-02-15 00:00:00pages
19-27issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(11)00611-Xjournal_volume
204pub_type
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