Stimulation of Ca²+ signals in neurons by electrically coupled electrolyte-oxide-semiconductor capacitors.

Abstract:

:Electrolyte-oxide-semiconductor capacitors (EOSCs) are a class of microtransducers for extracellular electrical stimulation that have been successfully employed to activate voltage-dependent sodium channels at the neuronal soma to generate action potentials in vitro. In the present work, we report on their use to control Ca²+ signalling in cultured mammalian cells, including neurons. Evidence is provided that EOSC stimulation with voltage waveforms in the microsecond or nanosecond range activates two distinct Ca²+ pathways, either by triggering Ca²+ entry through the plasma membrane or its release from intracellular stores. Ca²+ signals were activated in non-neuronal and neuronal cell lines, CHO-K1 and SH-SY5Y. On this basis, stimulation was tailored to rat and bovine neurons to mimic physiological somatic Ca²+ transients evoked by glutamate. Being minimally invasive and easy to use, the new method represents a versatile complement to standard electrophysiology and imaging techniques for the investigation of Ca²+ signalling in dissociated primary neurons and cell lines.

journal_name

J Neurosci Methods

authors

Giacomello M,Girardi S,Scorzeto M,Peruffo A,Maschietto M,Cozzi B,Vassanelli S

doi

10.1016/j.jneumeth.2011.02.009

subject

Has Abstract

pub_date

2011-05-15 00:00:00

pages

1-7

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(11)00084-7

journal_volume

198

pub_type

杂志文章
  • Detection of cerebral arterial gas embolism using regional cerebral oxygen saturation, quantitative electroencephalography, and brain oxygen tension in the swine.

    abstract:BACKGROUND:Cerebral air emboli occur as a complication of invasive medical procedures. The sensitivity of cerebral monitoring methods for the detection of air emboli is not known. This study investigates the utility of electroencephalography and non-invasively measured cerebral oxygen saturation in the detection of int...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2014.03.009

    authors: Weenink RP,Hollmann MW,Stevens MF,Kager J,van Gulik TM,van Hulst RA

    更新日期:2014-05-15 00:00:00

  • GAIN: A graphical method to automatically analyze individual neurite outgrowth.

    abstract:BACKGROUND:Neurite outgrowth is a metric widely used to assess the success of in vitro neural stem cell differentiation or neuron reprogramming protocols and to evaluate high-content screening assays for neural regenerative drug discovery. However, neurite measurements are tedious to perform manually, and there is a pa...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2017.03.013

    authors: Long BL,Li H,Mahadevan A,Tang T,Balotin K,Grandel N,Soto J,Wong SY,Abrego A,Li S,Qutub AA

    更新日期:2017-05-01 00:00:00

  • Methodological approaches to exploring epileptic disorders in the human brain in vitro.

    abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneumeth.2006.04.009

    authors: Köhling R,Avoli M

    更新日期:2006-07-15 00:00:00

  • Database tools for integrating and searching membrane property data correlated with neuronal morphology.

    abstract::A critical problem in neuroscience is the lack of database tools for integrating neuronal property data. We report here the development of a combined object oriented-relational database (NeuronDB, http://senselab.med.yale.edu/neurondb) that meets these needs by providing tools for integrating data within neurons and c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(98)00049-1

    authors: Mirsky JS,Nadkarni PM,Healy MD,Miller PL,Shepherd GM

    更新日期:1998-07-01 00:00:00

  • Machine learning on brain MRI data for differential diagnosis of Parkinson's disease and Progressive Supranuclear Palsy.

    abstract:BACKGROUND:Supervised machine learning has been proposed as a revolutionary approach for identifying sensitive medical image biomarkers (or combination of them) allowing for automatic diagnosis of individual subjects. The aim of this work was to assess the feasibility of a supervised machine learning algorithm for the ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.11.016

    authors: Salvatore C,Cerasa A,Castiglioni I,Gallivanone F,Augimeri A,Lopez M,Arabia G,Morelli M,Gilardi MC,Quattrone A

    更新日期:2014-01-30 00:00:00

  • The extraction of motion-onset VEP BCI features based on deep learning and compressed sensing.

    abstract:BACKGROUND:Motion-onset visual evoked potentials (mVEP) can provide a softer stimulus with reduced fatigue, and it has potential applications for brain computer interface(BCI)systems. However, the mVEP waveform is seriously masked in the strong background EEG activities, and an effective approach is needed to extract t...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2016.11.002

    authors: Ma T,Li H,Yang H,Lv X,Li P,Liu T,Yao D,Xu P

    更新日期:2017-01-01 00:00:00

  • An optimized method for enumerating CNS derived memory B cells during viral-induced inflammation.

    abstract:BACKGROUND:CNS inflammation resulting from infection, injury, or neurodegeneration leads to accumulation of diverse B cell subsets. Although antibody secreting cells (ASC) within the inflamed CNS have been extensively examined, memory B cell (Bmem) characterization has been limited as they do not secrete antibody witho...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2017.05.011

    authors: DiSano KD,Stohlman SA,Bergmann CC

    更新日期:2017-06-15 00:00:00

  • Medium-retaining Petri dish insert to grow and image cultured cells.

    abstract:BACKGROUND:Microscope chambers that accept glass coverslips with cultured cells are often used to monitor intracellular Ca2+ concentration ([Ca2+]i) during cell superfusion. Unfortunately, the experimental maneuvers associated with the coverslip installation in these chambers (medium removal and re-application) trigger...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2017.11.007

    authors: Kiedrowski L,Feinerman A

    更新日期:2018-01-15 00:00:00

  • Odor-induced emotion recognition based on average frequency band division of EEG signals.

    abstract:BACKGROUND:Emotion recognition plays a key role in multimedia. To enhance the sensation of reality, smell has been incorporated into multimedia systems because it can directly stimulate memories and trigger strong emotions. NEW METHOD:For the recognition of olfactory-induced emotions, this study explored a combination...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108599

    authors: Hou HR,Zhang XN,Meng QH

    更新日期:2020-01-21 00:00:00

  • A vapor exposure method for delivering heroin alters nociception, body temperature and spontaneous activity in female and male rats.

    abstract:BACKGROUND:The ongoing crisis related to non-medical use of opioids makes it of continued importance to understand the risk factors for opioid addiction, the behavioral and neurobiological consequences of opioid exposure and to seek potential avenues for therapy. Pre-clinical rodent models have been critical to advanci...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108993

    authors: Gutierrez A,Creehan KM,Taffe MA

    更新日期:2021-01-15 00:00:00

  • Randomising stimulus intensity improves the variability and reliability of the assessment of corticospinal excitability.

    abstract:BACKGROUND:Advances in the control of transcranial magnetic stimulation (TMS) have enabled greater randomisation of stimulus intensity. It is unclear if such randomisation improves assessments of corticospinal excitability. NEW METHOD:We recorded the amplitude of TMS-induced motor evoked potentials (MEPs) from the fir...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108813

    authors: Suckley JJ,Waters TJ,Tran M,Stapley PJ,Shemmell J,Walsh JA,McAndrew DJ

    更新日期:2020-08-01 00:00:00

  • A non-parametric Bayesian approach for clustering and tracking non-stationarities of neural spikes.

    abstract:BACKGROUND:Neural spikes from multiple neurons recorded in a multi-unit signal are usually separated by clustering. Drifts in the position of the recording electrode relative to the neurons over time cause gradual changes in the position and shapes of the clusters, challenging the clustering task. By dividing the data ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.12.005

    authors: Shalchyan V,Farina D

    更新日期:2014-02-15 00:00:00

  • Overexpression of growth-associated proteins in the neurons of adult transgenic mice.

    abstract::The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice. Transgene expression begins at P6-10, at a time when it can affect activity-dependent rearrangements of synaptic connections and neuron-glia interactions du...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0165-0270(96)00121-5

    authors: Caroni P

    更新日期:1997-01-01 00:00:00

  • High throughput quantification of mutant huntingtin aggregates.

    abstract::Mutant protein aggregates are an important biomarker in Huntington's and other neurodegenerative diseases however their quantification has typically relied on manual imaging and counting, or cell-free assays, which do not allow for concurrent analysis of cell viability. Here we describe four automated high throughput ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2008.02.007

    authors: Scotter EL,Narayan P,Glass M,Dragunow M

    更新日期:2008-06-15 00:00:00

  • Time-frequency analysis of resting state and evoked EEG data recorded at higher magnetic fields up to 9.4 T.

    abstract:BACKGROUND:Combining both high temporal and spatial resolution by means of simultaneous electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) is of relevance to neuroscientists. This combination, however, leads to a distortion of the EEG signal by the so-called cardio-ballistic artefacts. The ai...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2015.07.011

    authors: Abbasi O,Dammers J,Arrubla J,Warbrick T,Butz M,Neuner I,Shah NJ

    更新日期:2015-11-30 00:00:00

  • Numerical simulation of nonlinear feedback model of saccade generation circuit implemented in the LabView graphical programming language.

    abstract::The object-oriented graphical programming language LabView was used to implement the numerical solution to a computational model of saccade generation in primates. The computational model simulates the activity and connectivity of anatomical strictures known to be involved in saccadic eye movements. The LabView progra...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(98)00171-x

    authors: Jackson ME,Gnadt JW

    更新日期:1999-03-01 00:00:00

  • A framework on surface-based connectivity quantification for the human brain.

    abstract::Quantifying the connectivity between arbitrary surface patches in the human brain cortex can be used in studies on brain function and to characterize clinical diseases involving abnormal connectivity. Cortical regions of human brain in their natural forms can be represented in surface formats. In this paper, we presen...

    journal_title:Journal of neuroscience methods

    pub_type: 临床试验,杂志文章

    doi:10.1016/j.jneumeth.2011.02.017

    authors: Huang H,Prince JL,Mishra V,Carass A,Landman B,Park DC,Tamminga C,King R,Miller MI,van Zijl PC,Mori S

    更新日期:2011-04-30 00:00:00

  • A novel robotic system for quantifying arm kinematics and kinetics: description and evaluation in therapist-assisted passive arm movements post-stroke.

    abstract::We developed a system for quantitatively measuring arm movement. Our approach provides a method to simultaneously capture upper limb kinetic and kinematic data during assisted passive arm movements. Data are analysed with respect to Cartesian and upper limb coordinate systems to obtain upper limb joint angles and torq...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2011.03.004

    authors: Culmer PR,Jackson AE,Makower SG,Cozens JA,Levesley MC,Mon-Williams M,Bhakta B

    更新日期:2011-04-30 00:00:00

  • A novel method for removal of deep brain stimulation artifact from electroencephalography.

    abstract:BACKGROUND:Deep brain stimulation (DBS) has treatment efficacy in neurological and psychiatric disorders such as Parkinson's disease and major depression. Electroencephalography (EEG) is a versatile neurophysiological tool that can be used to better understand DBS treatment mechanisms. DBS causes artifacts in EEG recor...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2014.09.002

    authors: Sun Y,Farzan F,Garcia Dominguez L,Barr MS,Giacobbe P,Lozano AM,Wong W,Daskalakis ZJ

    更新日期:2014-11-30 00:00:00

  • Resolution of the stereoisomers of baclofen by high performance liquid chromatography.

    abstract::The GABA analogue baclofen [3-(p-chlorophenyl)-4-aminobutanoic acid] has stereospecific actions on the peripheral and central nervous systems. This paper describes the resolution of tritium-labelled baclofen by high performance liquid chromatography on a reverse-phase C18 column using a chiral mobile phase. The method...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(84)90076-1

    authors: Weatherby RP,Allan RD,Johnston GA

    更新日期:1984-01-01 00:00:00

  • Towards real-time detection of seizures in awake rats with GPU-accelerated diffuse optical tomography.

    abstract:BACKGROUND:Advancement in clinically relevant studies like seizure interruption using functional neuro imaging tools has shown that specific changes in hemodynamics precede and accompany seizure onset and propagation. However, preclinical seizure experiments need to be conducted in awake animals with images reconstruct...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2014.10.018

    authors: Zhang T,Zhou J,Carney PR,Jiang H

    更新日期:2015-01-30 00:00:00

  • In vivo cell type-specific CRISPR gene editing for sleep research.

    abstract::Sleep is an innate behavior conserved in all animals and, in vertebrates, is regulated by neuronal circuits in the brain. The conventional techniques of forward and reverse genetics have enabled researchers to investigate the molecular mechanisms that regulate sleep and arousal. However, functional interrogation of ge...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneumeth.2018.10.016

    authors: Yamaguchi H,de Lecea L

    更新日期:2019-03-15 00:00:00

  • Comparative one- and two-photon uncaging of MNI-glutamate and MNI-kainate on hippocampal CA1 neurons.

    abstract:BACKGROUND:The light-induced release of neurotransmitters from caging chromophores provides a powerful means to study the underlying receptors in a physiologically relevant context. Surprisingly, most caged neurotransmitters, including the widely used 4-methoxy-7-nitroindolinyl (MNI)-glutamate, show strong antagonism a...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2017.10.013

    authors: Passlick S,Ellis-Davies GCR

    更新日期:2018-01-01 00:00:00

  • Advances in the automation of whole-cell patch clamp technology.

    abstract::Electrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale. Despite great progress in imaging technologies and sensor proteins, no...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneumeth.2019.108357

    authors: Suk HJ,Boyden ES,van Welie I

    更新日期:2019-10-01 00:00:00

  • Relation between myelin area and axon diameter in the aortic depressor nerve of spontaneously hypertensive rats.

    abstract::The hypothesis that the aortic depressor nerve (ADN) from spontaneously hypertensive rats (SHR) does not show the expected correlation between myelin sheath area and the axonal diameter of myelinated fibers detected in normotensive rat myelinated fibers was tested by means of regression analysis. Proximal and distal s...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2005.04.007

    authors: Fazan VP,Salgado HC,dos Reis GC,Barreira AA

    更新日期:2005-10-30 00:00:00

  • Detection of cytochrome c oxidase activity and mitochondrial proteins in single cells.

    abstract::Cytochrome c oxidase or mitochondrial respiratory chain complex IV is where over 90% of oxygen is consumed. The relationship between complex IV activity and mitochondrial proteins, which provides a guide to understanding the mechanisms in primary mitochondrial disorders, has been determined by histochemistry (activity...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2009.08.020

    authors: Mahad DJ,Ziabreva I,Campbell G,Laulund F,Murphy JL,Reeve AK,Greaves L,Smith KJ,Turnbull DM

    更新日期:2009-11-15 00:00:00

  • Synaptic plasticity in the hippocampal slice: functional consequences.

    abstract::There are 3 known forms of synaptic plasticity at CNS synapses: long-term potentiation (LTP) mediated by NMDA receptor activation, LTP mediated by voltage-dependent calcium channel (VDCC) activation, and long-term depression (LTD) mediated by the NMDA receptor. All 3 forms of synaptic plasticity can be observed in hip...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(94)00188-m

    authors: Teyler TJ,Cavus I,Coussens C

    更新日期:1995-06-01 00:00:00

  • Electrochemical monitoring of transport by a vesicular monoamine transporter expressed in Xenopus oocytes.

    abstract::Xenopus laevis oocytes were injected with synthetic mRNA coding for a rat VMAT2 mutant (rVMAT2-I483A/L484A) shown previously to be retained on the plasma membrane as a result of a presumed reduction of endocytosis. Binding of the specific VMAT inhibitor [3H]dihydrotetrabenazine indicated that expression did occur at a...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2003.10.010

    authors: Whitley J,Parsons J,Freeman J,Liu Y,Edwards RH,Near JA

    更新日期:2004-02-15 00:00:00

  • Extracting multi-mode ERP features using fifth-order nonnegative tensor decomposition.

    abstract:BACKGROUND:Preprocessed Event-related potential (ERP) data are usually organized in multi-way tensor, in which tensor decomposition serves as a powerful tool for data processing. Due to the limitation of computation burden for multi-way data and the low algorithm performance of stability and efficiency, multi-way ERP d...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2018.07.020

    authors: Wang D,Zhu Y,Ristaniemi T,Cong F

    更新日期:2018-10-01 00:00:00

  • Intravenous recording of intracranial, broadband EEG.

    abstract::The most direct evaluation of human brain activity has been obtained from intracranial electrodes placed either on the surface of the brain or inserted into the brain to record from deep brain structures. Currently, the placement of intracranial electrodes implies transcranial surgery, either through a burr hole or a ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2012.12.027

    authors: Bower MR,Stead M,Van Gompel JJ,Bower RS,Sulc V,Asirvatham SJ,Worrell GA

    更新日期:2013-03-30 00:00:00