Abstract：
:Modern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provide unforeseen insights into the complexities of the nervous system. While several iterations of optical clearing techniques have been developed, some are more suitable for specific applications than others depending on the type of specimen under study. Here we review findings from select studies utilizing clearing methods to visualize the developing, injured, and diseased nervous system within numerous model systems and species. We note trends and imbalances in the types of research questions being addressed with clearing methods across these fields in neuroscience. In addition, we discuss restrictions in applying optical clearing methods for postmortem tissue from humans and large animals and emphasize the lack in continuity between studies of these species. We aim for this review to serve as a key outline of available tissue clearing methods used successfully to address issues across neuronal development, injury/repair, and aging/disease.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Porter DDL,Morton PDdoi
10.1016/j.jneumeth.2020.108594subject
Has Abstractpub_date
2020-01-13 00:00:00pages
108594eissn
0165-0270issn
1872-678Xpii
S0165-0270(20)30016-9journal_volume
334pub_type
杂志文章,评审abstract：:The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations or pharmacological manipulations. Therefore, a method using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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