Abstract:
:Neurophysiologists have been investigating the responses of neurons in the visual system for the past half-century using monkeys and cats that are anesthetized and paralyzed, with the non-blinking eyelids open for prolonged periods of time. Impermeable plastic contact lenses have been used to prevent dehydration of the corneal epithelium, which would otherwise occur in minutes. Unfortunately, such lenses rapidly introduce a variety of abnormal states that lead to clouding of the cornea, degradation of the retinal image, and premature termination of the experiment. To extend the viability of such preparations, a new protocol for maintenance of corneal health has been developed. The protocol uses rigid gas permeable contact lenses designed to maximize gas transmission, rigorous sterile methods, and a variety of methods for sustaining and monitoring the overall physiology of the animal. The effectiveness of the protocol was evaluated clinically by ophthalmoscopy before, during, and after the experiments, which lasted 8-10 days. Histopathology and quantitative histology were performed on the corneas following the experiment. Our observations showed that this protocol permits continuous contact lens wear without adversely affecting the corneas. Thus, it is possible to collect data 24 h each day, for the entire duration of the experiment.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Metha AB,Crane AM,Rylander HG 3rd,Thomsen SL,Albrecht DGdoi
10.1016/s0165-0270(01)00409-5subject
Has Abstractpub_date
2001-08-30 00:00:00pages
153-66issue
2eissn
0165-0270issn
1872-678Xpii
S0165027001004095journal_volume
109pub_type
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