A low-cost, portable, micro-controlled device for multi-channel LED visual stimulation.

Abstract:

:Light emitting diodes (LEDs) are extensively used as light sources to investigate visual and visually related function and dysfunction. Here, we describe the design of a compact, low-cost, stand-alone LED-based system that enables the configuration, storage and presentation of elaborate visual stimulation paradigms. The core functionality of this system is provided by a microcontroller whose ultra-low power consumption makes it well suited for long lasting battery applications. The effective use of hardware resources is managed by multi-layered architecture software that provides an intuitive and user-friendly interface. In the configuration mode, different stimulation sequences can be created and memorized for ten channels, independently. LED-driving current output can be set either as continuous or pulse modulated, up to 500 Hz, by duty cycle adjustments. In run mode, multiple-channel stimulus sequences are automatically applied according to the pre-programmed protocol. Steady state visual evoked potentials were successfully recorded in five subjects with no visible electromagnetic interferences from the stimulator, demonstrating the efficacy of combining our prototyped equipment with electrophysiological techniques. Finally, we discuss a number of possible improvements for future development of our project.

journal_name

J Neurosci Methods

authors

Pinto MA,de Souza JK,Baron J,Tierra-Criollo CJ

doi

10.1016/j.jneumeth.2011.02.004

subject

Has Abstract

pub_date

2011-04-15 00:00:00

pages

82-91

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(11)00079-3

journal_volume

197

pub_type

杂志文章
  • Assessment of executive function in adolescence: a comparison of traditional and virtual reality tools.

    abstract::Paper-pencil type tests are traditionally used in the assessment of executive functions (EF); however, concerns have been raised as to whether these represent actual functioning in everyday life. Virtual reality (VR) environments offer a novel alternative for the assessment of cognitive function and therefore have the...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.07.005

    authors: Lalonde G,Henry M,Drouin-Germain A,Nolin P,Beauchamp MH

    更新日期:2013-09-30 00:00:00

  • Feasibility of electrical recordings from unconnected vertebrate CNS neurons cultured in a three-dimensional extracellular matrix.

    abstract::Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(87)90052-5

    authors: Coates PW,Nathan RD

    更新日期:1987-07-01 00:00:00

  • The role of diversity in complex ICA algorithms for fMRI analysis.

    abstract:BACKGROUND:The widespread use of data-driven methods, such as independent component analysis (ICA), for the analysis of functional magnetic resonance imaging data (fMRI) has enabled deeper understanding of neural function. However, most popular ICA algorithms for fMRI analysis make several simplifying assumptions, thus...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2016.03.012

    authors: Du W,Levin-Schwartz Y,Fu GS,Ma S,Calhoun VD,Adalı T

    更新日期:2016-05-01 00:00:00

  • Three-dimensional reconstruction: a tissue embedding method for alignment of serial sections.

    abstract::Three-dimensional (3D) reconstruction from serial sections that are undistorted and true-to-life is only possible when a reference system exists that allows correct matching of the section drawings. This paper describes a simple method for producing reference lines that ensures proper alignment of the drawings. (1) Th...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(88)90162-8

    authors: Meyer EP,Domanico VJ

    更新日期:1988-12-01 00:00:00

  • A portable chamber for long-distance transport of surviving human brain slice preparations.

    abstract::Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(96)00072-6

    authors: Köhling R,Lücke A,Straub H,Speckmann EJ

    更新日期:1996-08-01 00:00:00

  • A reliable method for culture of dissociated mouse cerebellar cells enriched for Purkinje neurons.

    abstract::The cerebellar Purkinje neuron (PN) serves as an important model in studies of neuronal development in the mammalian central nervous system. Dissociated PN preparations maintained in an in-vitro environment with simplified cellular and biochemical conditions can facilitate molecular analyses of neuronal development. H...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(00)00323-x

    authors: Tabata T,Sawada S,Araki K,Bono Y,Furuya S,Kano M

    更新日期:2000-12-15 00:00:00

  • Turbulence in globus pallidum neurons in patients with Parkinson's disease: exponential decay of the power spectrum.

    abstract::Exponential decay of the power spectrum is typically observed in turbulent flow patterns, as evidenced both in experiments and in the Navier-Stokes equations describing fluid dynamics. In this study, we present evidence on the exponential decay of power spectra belonging to interspike interval time series, registered ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2011.01.022

    authors: Andres DS,Cerquetti DF,Merello M

    更新日期:2011-04-15 00:00:00

  • Analysis of the growth cone turning assay for studying axon guidance.

    abstract::The "pipette" or "growth cone turning" assay is widely used for studying how axons respond to diffusible guidance cues in their environment. However, little quantitative analysis has been presented of the gradient shapes produced by this assay, or how they depend on parameters of the assay. Here we used confocal micro...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2008.01.014

    authors: Pujic Z,Giacomantonio CE,Unni D,Rosoff WJ,Goodhill GJ

    更新日期:2008-05-30 00:00:00

  • Odor-induced emotion recognition based on average frequency band division of EEG signals.

    abstract:BACKGROUND:Emotion recognition plays a key role in multimedia. To enhance the sensation of reality, smell has been incorporated into multimedia systems because it can directly stimulate memories and trigger strong emotions. NEW METHOD:For the recognition of olfactory-induced emotions, this study explored a combination...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108599

    authors: Hou HR,Zhang XN,Meng QH

    更新日期:2020-01-21 00:00:00

  • A reliable method for Golgi staining of retina and brain slices.

    abstract::Although the classical Golgi method is a powerful means for structural analysis of the brain, it is generally considered to be an unpredictable technique making anatomists wary of using it. Often, even when successful staining has occurred, deposits of silver chromate crystals on the surface of the tissue obscure exam...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(95)00160-3

    authors: Angulo A,Fernández E,Merchán JA,Molina M

    更新日期:1996-05-01 00:00:00

  • Amperometric analysis of exocytosis at chromaffin cells from genetically distinct mice.

    abstract::Amperometry is a very powerful technique for investigating the role(s) specific proteins play in exocytosis at the single-cell level. In this study, amperometry has been used to investigate possible changes in exocytosis at chromaffin cells isolated from coloboma and tottering mutant mice. Coloboma mice possess a dele...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(00)00359-9

    authors: Colliver TL,Hess EJ,Ewing AG

    更新日期:2001-01-30 00:00:00

  • New software dedicated to virtual mazes for human cognitive investigations.

    abstract:BACKGROUND:Compared to previous neuropsychological investigations with standard paper-pen tests limited to test complex spatial learning and memory processes, 3-D virtual immersive technology might offer new tools for research purposes and for diagnosis in patients suffering from mild cognitive impairment or dementia. ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2019.108388

    authors: Machado ML,Lefèvre N,Philoxene B,Le Gall A,Madeleine S,Fleury P,Smith PF,Besnard S

    更新日期:2019-11-01 00:00:00

  • Calibrating balance perturbation using electrical stimulation of the vestibular system.

    abstract:BACKGROUND:Supra-threshold galvanic vestibular stimulation (GVS) can be used to challenge the balance control system by disrupting vestibular inputs. The goal of this study was to propose an objective method to assess variability across subjects in the minimum safe GVS level that causes maximum balance degradation. New...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2018.10.012

    authors: Goel R,Rosenberg MJ,Cohen HS,Bloomberg JJ,Mulavara AP

    更新日期:2019-01-01 00:00:00

  • Overexpression of growth-associated proteins in the neurons of adult transgenic mice.

    abstract::The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice. Transgene expression begins at P6-10, at a time when it can affect activity-dependent rearrangements of synaptic connections and neuron-glia interactions du...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/s0165-0270(96)00121-5

    authors: Caroni P

    更新日期:1997-01-01 00:00:00

  • A kernel-based method to calculate local field potentials from networks of spiking neurons.

    abstract:BACKGROUND:The local field potential (LFP) is usually calculated from current sources arising from transmembrane currents, in particular in asymmetric cellular morphologies such as pyramidal neurons. NEW METHOD:Here, we adopt a different point of view and relate the spiking of neurons to the LFP through efferent synap...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108871

    authors: Telenczuk B,Telenczuk M,Destexhe A

    更新日期:2020-10-01 00:00:00

  • Utilization of in situ ELISA method for examining Trk receptor phosphorylation in cultured cells.

    abstract:BACKGROUND:Trk receptor tyrosine kinases regulate multiple important neuronal processes during the development and in the adulthood. Tyrosine phosphorylation of Trk serves as the initial step in the Trk signaling pathway and indicates receptor' autocatalytic activity. However, methods allowing simple and large-scale Tr...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.11.001

    authors: Antila H,Autio H,Turunen L,Harju K,Tammela P,Wennerberg K,Yli-Kauhaluoma J,Huttunen HJ,Castrén E,Rantamäki T

    更新日期:2014-01-30 00:00:00

  • A method to evaluate the diffusion rate of drugs from a microdialysis probe through brain tissue.

    abstract::For interpretation of microdialysis experiments in which compounds are applied retrodialysis, it is important to have information about the migration rate of the infused compounds. Here we describe a dual-probe microdialysis method that can be used to evaluate the penetration rate of the infused drug. The basic idea i...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(01)00401-0

    authors: Westerink BH,De Vries JB

    更新日期:2001-08-15 00:00:00

  • A simplified method for selecting a carbon-fiber electrode in pulse voltammetry.

    abstract::A method for selecting a usable carbon-fiber electrode using the equivalent resistance and capacitance is presented. This method uses an instrument with a PC-based look-up table for measuring the electrical characteristics of a carbon-fiber electrode in pulse voltammetry. Using this instrument, the equivalent resistan...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(93)90036-q

    authors: Liao BY,Lio HP,Wang CY,Young MS,Ho MT,Lin MT

    更新日期:1993-12-01 00:00:00

  • Quantifying periodic activity in central pattern generators: the crayfish swimmeret.

    abstract::Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(93)90053-t

    authors: Olsen OH,Murray-Smith D

    更新日期:1993-10-01 00:00:00

  • Optimization of differential immunogold-silver and peroxidase labeling with maintenance of ultrastructure in brain sections before plastic embedding.

    abstract::The limited success of immunogold labeling for pre-embedding immunocytochemistry of neuronal antigens is largely attributed to poor penetration of large (5-20 nm) colloidal gold particles. We examined the applicability of using silver intensification of 1 nm colloidal gold particles non-covalently bound to goat anti-r...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(90)90015-8

    authors: Chan J,Aoki C,Pickel VM

    更新日期:1990-08-01 00:00:00

  • Brain dialysis of neurotransmitters: a commentary.

    abstract::Brain dialysis has become an important tool for investigating changes in the extracellular levels of neurotransmitters. This paper reviews the experimental variables and criteria that should be considered when interpreting data obtained with the brain dialysis method. Brain dialysis can provide important direct inform...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/0165-0270(90)90039-i

    authors: Di Chiara G

    更新日期:1990-09-01 00:00:00

  • Quantitative analysis of acetylcholine in rat brain microdialysates by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

    abstract::A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2010.09.016

    authors: Carrozzo MM,Cannazza G,Pinetti D,Di Viesti V,Battisti U,Braghiroli D,Parenti C,Baraldi M

    更新日期:2010-12-15 00:00:00

  • Detecting brain structural changes as biomarker from magnetic resonance images using a local feature based SVM approach.

    abstract::Detecting brain structural changes from magnetic resonance (MR) images can facilitate early diagnosis and treatment of neurological and psychiatric diseases. Many existing methods require an accurate deformation registration, which is difficult to achieve and therefore prevents them from obtaining high accuracy. We de...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.09.001

    authors: Chen Y,Storrs J,Tan L,Mazlack LJ,Lee JH,Lu LJ

    更新日期:2014-01-15 00:00:00

  • Object recognition analysis in mice using nose-point digital video tracking.

    abstract::Preferential exploration of novel locations and objects by rodents has been used to test the effects of various manipulations on object recognition memory. However, manual scoring is time-consuming, requires extensive training, and is subject to inter-observer variability. Since rodents explore primarily by sniffing, ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2007.11.002

    authors: Benice TS,Raber J

    更新日期:2008-03-15 00:00:00

  • Caloric stimulation of ampullar receptors: a new method to produce mechanically-evoked responses in frog semicircular canals.

    abstract::A microthermistor positioned close to the exposed posterior semicircular canal in isolated labyrinth preparations of the frog was used to stimulate the sensory organ. Our results indicated that, depending on the position of the heater, the induced endolymphatic convection currents may result in either excitatory or in...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(99)00021-7

    authors: Zucca G,Botta L,Valli S,Giannoni B,Mira E,Perin P,Valli P

    更新日期:1999-05-01 00:00:00

  • Determination of in vivo amino acid neurotransmitters by high-performance liquid chromatography with o-phthalaldehyde-sulphite derivatisation.

    abstract::The measurement of amino acid neurotransmitters by high-performance liquid chromatography (HPLC) has emerged as a reliable and sensitive method. This paper describes a method which employs electrochemical (EC) detection of amino acid derivatives formed by a reaction with o-phthalaldehyde (OPA) in the presence of sulph...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(94)00132-z

    authors: Rowley HL,Martin KF,Marsden CA

    更新日期:1995-03-01 00:00:00

  • A new algorithm for drift compensation in multi-unit recordings of action potentials in peripheral autonomic nerves over time.

    abstract:BACKGROUND:Peripheral autonomic nerves control visceral organs and convey information regarding their functional states and are, therefore, potential targets for new therapeutic and diagnostic approaches. Conventionally recorded multi-unit nerve activity in vivo undergoes slow differential drift of signal and noise amp...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneumeth.2020.108683

    authors: Davey CE,Soto-Breceda A,Shafton A,McAllen RM,Furness JB,Grayden DB,Stebbing MJ

    更新日期:2020-05-15 00:00:00

  • Cleaning MEG artifacts using external cues.

    abstract::Using EEG, ECoG, MEG, and microelectrodes to record brain activity is prone to multiple artifacts. The main power line (mains line), video equipment, mechanical vibrations and activities outside the brain are the most common sources of artifacts. MEG amplitudes are low, and even small artifacts distort recordings. In ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.04.002

    authors: Tal I,Abeles M

    更新日期:2013-07-15 00:00:00

  • Consistency of independent component analysis for FMRI.

    abstract:BACKGROUND:Independent component analysis (ICA) has been widely used for blind source separation in the field of medical imaging. However, despite of previous substantial efforts, the stability of ICA components remains a critical issue which has not been adequately addressed, despite numerous previous efforts. Most cr...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.109013

    authors: Zhao W,Li H,Hu G,Hao Y,Zhang Q,Wu J,Frederick BB,Cong F

    更新日期:2020-12-11 00:00:00

  • A microprocessor-based tissue displacement monitor for in vitro quantification of knee capsule deformation.

    abstract::The advantages of utilizing a microprocessor design kit as a central element in custom laboratory instrumentation are demonstrated in the design of a real-time, multiple point, tissue displacement monitor. The tissue sample to be monitored is submerged in a saline bath, through which perpendicular electric fields are ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(84)90066-9

    authors: Looft FJ,Lyons HD

    更新日期:1984-02-01 00:00:00