Abstract：
BACKGROUND:Polarized light imaging (PLI) has evolved into a powerful neuroimaging tool to analyze fiber tracts with submillimeter resolution in microtome sections of postmortem human brain tissue. In PLI polarized light changes its polarization state while passing through birefringent tissue, i.e., myelinated axons, which results in sinusoidal signals that characterize different fiber orientations. Noise, light scatter and filter inhomogeneities of the polarimeter interfere with the original sinusoidal PLI signals, which have direct effects on the accuracy of subsequent fiber modeling. New method: In our recent publications we have shown that the sinusoidal signal at each pixel location in PLI images can be restored utilizing independent component analysis (ICA). We now have further improved the signal separation quality by introducing a new constrained ICA algorithm (cICAP) where the component selection is directly included. In cICAP an analytical expression of the expected signal of interest is implemented as a priori information. RESULTS:The algorithm precisely decomposes the deteriorated PLI signals into its underlying source signals. As such, the approach enhances sinusoidal basis functions and is therefore optimal for the extraction of independent spatial maps from PLI images. Comparison with existing methods: The new algorithm performs better and is faster compared to other well-known ICA algorithms. CONCLUSION:The decomposition in cICAP is optimal with respect to separation and identification of the sinusoidal nature of the PLI signal. In this way the identification of the relevant components is automatically included and does not require any further component selection tool.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Breuer L,Axer M,Dammers Jdoi
10.1016/j.jneumeth.2013.08.022subject
Has Abstractpub_date
2013-10-30 00:00:00pages
30-8issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(13)00300-2journal_volume
220pub_type
杂志文章abstract：:We present a new analytical method for characterizing the directional tuning of neural data. The method is based on computing parameters associated with the geometric properties of solids, and provides an estimate of preferred direction in the context of non-uniform sampling of directions. Unlike optimization methods ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00492-7
更新日期：2002-01-30 00:00:00
abstract：:The number of studies examining cognition in Huntington's disease (HD) has increased dramatically in recent decades, and cognitive research methods in HD have become much more sophisticated. In this review, we provide a summary of the advances in cognitive research in HD to date, and outline the key considerations for...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.12.007
更新日期：2016-05-30 00:00:00
abstract：BACKGROUND:Recent advances in genetically engineered calcium and membrane potential indicators provide the potential to estimate the activation dynamics of individual neurons within larger, mesoscale networks (100s-1000+neurons). However, a fully integrated automated workflow for the analysis and visualization of neura...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.01.020
更新日期：2015-03-30 00:00:00
abstract：:We investigate the mode locking properties of simple dynamical models of pulse-coupled neurons to two tones, i.e., simple musical intervals. A recently proposed nonlinear synchronization theory of musical consonance links the subjective ranking from consonant to dissonant intervals to the universal ordering of robustn...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.041
更新日期：2009-09-30 00:00:00
abstract：:A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridiu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90131-8
更新日期：1988-11-01 00:00:00
abstract：:The study aim was to develop a model to generate cerebral evoked potentials (CEPs) by mechanical distention of the duodenum in rats. Twenty Sprague-Dawley rats were anaesthetized and the EEG recorded from the left and right somatosensory cortices (S1L, S1R). A balloon catheter was implanted into the duodenum. A pneuma...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.01.014
更新日期：2007-05-15 00:00:00
abstract：:The force-plate actometer is a relatively new computer-based instrument with high temporal and spatial resolution that has been used to measure the behavioral effects of genetic restriction (e.g., inbred mice) and drugs (e.g., dopaminergic agonists and antagonists) on a variety of behaviors in rodents, including locom...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.10.002
更新日期：2006-05-15 00:00:00
abstract：:We have developed a labeling procedure which accurately and consistently labels the original sensory pools projecting to their respective nerve branches as a model to quantify the accuracy of nerve regeneration at the single neuron level. Adult and juvenile rats had the saphenous branch of the femoral nerve transected...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90078-e
更新日期：1991-09-01 00:00:00
abstract：:The development of high-resolution retinal prostheses fabricated from silicon wafers presents an interesting problem: how to electrically bridge the space between the flat silicon wafer and the curved retinal surface. One potential "bridge" is a microwire glass electrode. In this paper we present our results in evalua...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.02.035
更新日期：2004-08-30 00:00:00
abstract：:A system for rapid solution exchange (concentration-clamp) was developed for Xenopus laevis oocytes. The exchange time (t90) was 10 ms. The system allows two-electrode voltage-clamp investigations. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90178-3
更新日期：1991-07-01 00:00:00
abstract：BACKGROUND:CNS inflammation resulting from infection, injury, or neurodegeneration leads to accumulation of diverse B cell subsets. Although antibody secreting cells (ASC) within the inflamed CNS have been extensively examined, memory B cell (Bmem) characterization has been limited as they do not secrete antibody witho...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.05.011
更新日期：2017-06-15 00:00:00
abstract：:Event related potentials (ERPs) are very feeble alterations in the ongoing electroencephalogram (EEG) and their detection is a challenging problem. Based on the unique time-based parameters derived from wavelet coefficients and the asymmetry property of wavelets a novel algorithm to separate ERP components in single-t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.06.004
更新日期：2014-08-15 00:00:00
abstract：:In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Im...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00211-9
更新日期：2000-06-30 00:00:00
abstract：:The neuromuscular junction (NMJ) in Drosophila larvae serves as an important model system to study synaptic vesicle recycling, plasticity, development, and organelle trafficking in nerve terminals. Here we provide a protocol that reliably localizes proteins subcellularly in this system in combination with good preserv...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.10.010
更新日期：2010-01-15 00:00:00
abstract：:We describe a relational database (RDB) structure suitable for trial-based experiments such as human psychophysics and neural recording studies in trained animals. An RDB is a collection of tables, each composed of columns. Some of the tables contain columns that reference specific columns of other tables. This refere...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.05.014
更新日期：2005-01-30 00:00:00
abstract：:We have developed a novel double-labeling method to investigate multiple gene expression in single cells. The method relies on the use of a radioactive probe followed by a colorimetric probe. Unique to this method, the radioactive signal is first captured on an emulsion pre-coated slide, which totally separates it fro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00163-8
更新日期：1996-06-01 00:00:00
abstract：BACKGROUND:Temporal changes of pain perception to prolonged tonic heat pain are conventionally assessed using a computerized visual analog scale. Such a rating-based approach is, however, prone to floor and ceiling effects, which limit the assessment of temporal changes in perception. Thus, alternative methods that ove...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.04.003
更新日期：2019-06-01 00:00:00
abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00262-5
更新日期：2002-12-15 00:00:00
abstract：:Slice cultures on biomembrane are the method of choice for studying Ca2+-dependent plastic changes occurring over several days to weeks. Using IR-differential interference contrast, good visualization of neurons in biomembrane slice cultures has been achieved despite a negative optical effect of the biomembrane, but e...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00148-1
更新日期：2003-09-30 00:00:00
abstract：:In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90179-3
更新日期：1988-03-01 00:00:00
abstract：:The process of peripheral nerve regeneration has been modeled using 5 populations of mathematical variables to represent the biological activities of Wallerian degeneration, fibrin matrix development, Schwann cell activity, elongating neurites, and neovascularization. The mathematical model provided simulations of ner...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00222-3
更新日期：1995-08-01 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) is an X-chromosome-linked disorder that arises from a mutation in the gene for the cytoskeletal protein dystrophin, normally expressed in the myofibres. The most widely applied animal model in DMD basic research is the C57BL/10ScSn-mdx/J mouse, commonly referred to as the "mdx mouse"....
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.10.001
更新日期：2012-01-30 00:00:00
abstract：:Human muscle fatigue has been studied using a wide variety of exercise models, protocols and assessment methods. Based on the definition of fatigue as 'any reduction in the maximal capacity to generate force or power output', the different methods to measure fatigue are discussed. It is argued that reliable and valid ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/s0165-0270(97)02251-6
更新日期：1997-06-27 00:00:00
abstract：:We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00313-7
更新日期：2000-11-30 00:00:00
abstract：:A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.03.021
更新日期：2004-09-30 00:00:00
abstract：BACKGROUND:Compared to previous neuropsychological investigations with standard paper-pen tests limited to test complex spatial learning and memory processes, 3-D virtual immersive technology might offer new tools for research purposes and for diagnosis in patients suffering from mild cognitive impairment or dementia. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108388
更新日期：2019-11-01 00:00:00
abstract：:Measurements of neurotransmitters in conscious animals have been restrictive in real-time. The use of specific enzymes within an amperometric probe, based upon a microdialysis membrane, may overcome some of these problems. I report the use of such a probe, with different perfusions of enzymes, to allow real-time measu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)02175-9
更新日期：1997-04-04 00:00:00
abstract：:In the present study the accumulation of protease resistant prion protein (PrPres) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest levels of PrPres were found in slow growing cells. Further increases in PrPres accumulation were observed in ScN2a cells ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.04.001
更新日期：2004-09-30 00:00:00
abstract：:Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00072-6
更新日期：1996-08-01 00:00:00
abstract：BACKGROUND:Independent component analysis (ICA) has been widely used for blind source separation in the field of medical imaging. However, despite of previous substantial efforts, the stability of ICA components remains a critical issue which has not been adequately addressed, despite numerous previous efforts. Most cr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109013
更新日期：2020-12-11 00:00:00
