Abstract:
:A rapid liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed for the measurement of dopamine (DA), 5-hydroxytryptamine (5HT) and norepinephrine (NE) in brain microdialysates. The assay has also been utilised for the simultaneous measurement of these neurotransmitters and cocaine in brain dialysates. The neurotransmitters and cocaine were resolved in a single 4-min run using a binary gradient elution profile. The analytes were detected using tandem mass spectrometry in the positive ion electrospray mode. The limits of detection for DA, NE, 5HT and cocaine were 200, 1000, 900 pM and 1 pg ml(-1), respectively.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Hows ME,Lacroix L,Heidbreder C,Organ AJ,Shah AJdoi
10.1016/j.jneumeth.2004.03.021subject
Has Abstractpub_date
2004-09-30 00:00:00pages
123-32issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165027004001281journal_volume
138pub_type
杂志文章abstract::The peptidome encompasses all the peptides present in a particular cell, tissue or organism at a particular point in time. Neuropeptidomics studies the peptidome of the nervous system and will become increasingly important in neuroscience research. Novel peptides can be discovered and, when applied to disease models, ...
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journal_title:Journal of neuroscience methods
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