Abstract：
:A simple method for making a stimulating multi-channel metallic electrode is proposed. The method uses furnace-shaped multiple fine glass tubes as channel guides for inserted tungsten wires that are pulled as a unit to produce multi-channel metallic electrode with a tapered tip. By shortening the tip, the desired inter-channels distances (from tens to hundreds of micrometers) can be established with a high degree of accuracy. Such an electrode was used to stimulate the layer of ganglion cells in a frog's retina. The responses to stimuli were recorded extracellularly in the contralateral tectum from the terminal arborizations of the excited ganglion cells' axons. A larger area of the retina may be excited when several channels are used as a cathode and several others as an anode. By varying the stimulating pairs of the channels it is possible to change the site of excitation without displacing the electrode. Separate ganglion cells may be stimulated, provided the appropriate pair of channels is selected. In this case 'all-or-none' responses to the threshold stimuli are obtained in the tectum. The artefact of the stimulus is diminished effectively by joining additional channels to the anodic one.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kuras A,Gutmaniene Ndoi
10.1016/s0165-0270(97)00063-0subject
Has Abstractpub_date
1997-07-18 00:00:00pages
99-102issue
1eissn
0165-0270issn
1872-678Xpii
S0165027097000630journal_volume
75pub_type
杂志文章abstract：BACKGROUND:Neuronal cell cultures are widely used in the field of neuroscience. Cell dissociation allows for the isolation of a desired cell type, yet the neuronal complexity that distinguishes the nervous system is often lost as a result. Thus, culturing neural tissues in ex vivo format provides a physiological contex...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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