A rapid fluorescent method to quantify neuronal loss after experimental intracerebral hemorrhage.

Abstract:

:Neuronal loss in tissue surrounding an intracerebral hemorrhage (ICH) is usually quantified by labor-intensive histological methods that are subject to bias. Fluorescent protein expression has been successfully used as a marker of cell viability in vitro and in retinal studies in vivo, but not in any ICH model to date. The potential of this approach was investigated using transgenic mice that constitutively express the red fluorescent protein variant dTomato in central neurons under the control of the Thy1 promoter. Breeding and growth of these mice were similar to their wild-type counterparts; behavioral phenotyping by digital analysis of home cage video recordings detected no differences. Bright fluorescence was evident in fresh brain samples with minimal background fluorescence, and was reduced in tissue surrounding the hematoma. In order to assess fluorescence loss as an injury marker in a planned study, these mice were crossed with heme oxygenase (HO)-2 knockouts and wild-type controls; striatal hemorrhage was induced by stereotactic injection of collagenase. Fluorescence in hemorrhagic striata was reduced to 86.4±3.9%, 62.2±5.1%, and 58.3±3.0% of contra-lateral on days 1, 4 and 8, respectively, and correlated closely with reduction in striatal cell viability as quantified by MTT assay. HO-2 knockout and wild-type values did not differ significantly. Similar results were observed with stereological cell counts of striatal neurons identified by NeuN immunoreactivity. These results suggest that loss of constitutive dTomato fluorescence is an accurate and efficient marker of neuronal loss in tissue surrounding a striatal hematoma.

journal_name

J Neurosci Methods

authors

Chen-Roetling J,Lu X,Regan KA,Regan RF

doi

10.1016/j.jneumeth.2013.03.025

subject

Has Abstract

pub_date

2013-06-15 00:00:00

pages

128-36

issue

2

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(13)00130-1

journal_volume

216

pub_type

杂志文章
  • Osprey: Open-source processing, reconstruction & estimation of magnetic resonance spectroscopy data.

    abstract:BACKGROUND:Processing and quantitative analysis of magnetic resonance spectroscopy (MRS) data are far from standardized and require interfacing with third-party software. Here, we present Osprey, a fully integrated open-source data analysis pipeline for MRS data, with seamless integration of pre-processing, linear-comb...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108827

    authors: Oeltzschner G,Zöllner HJ,Hui SCN,Mikkelsen M,Saleh MG,Tapper S,Edden RAE

    更新日期:2020-09-01 00:00:00

  • Transcardial perfusion is not required to accurately measure cytokines within the brain.

    abstract:BACKGROUND:Cytokines are key signaling molecules within the immune system that regulate a host's response to pathogens and neuronal damage. Aberrant cytokine signaling has been implicated in many neurological diseases. Therefore, accurately measuring cytokine concentrations within the brain is crucial. NEW METHOD:This...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108601

    authors: Walker WH 2nd,Bumgarner JR,Nelson RJ,Courtney DeVries A

    更新日期:2020-01-22 00:00:00

  • The use of in vivo direct drug application to assess neural regulation of muscle properties.

    abstract::Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(00)00352-6

    authors: Buffelli M,Busetto G,Cangiano A

    更新日期:2001-04-30 00:00:00

  • A method for collecting semithin epoxy serial sections for light microscopy and 3-D reconstruction.

    abstract::In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(88)90179-3

    authors: Wali N,Jagadeesh JM

    更新日期:1988-03-01 00:00:00

  • Method for in situ detection of the mitochondrial function in neurons.

    abstract::Conventional studies of neuronal mitochondria have been limited to the use of purified preparations of isolated mitochondria, neural cell homogenates, living neurons, or brain slices. However, each technique has several drawbacks. Here, we demonstrate that the neuronal cell's membrane can be effectively permeabilized ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2004.02.027

    authors: Safiulina D,Kaasik A,Seppet E,Peet N,Zharkovsky A,Seppet E

    更新日期:2004-08-15 00:00:00

  • Cryopreserved rat cortical cells develop functional neuronal networks on microelectrode arrays.

    abstract::Neurons growing on microelectrode arrays (MEAs) are promising tools to investigate principal neuronal network mechanisms and network responses to pharmaceutical substances. However, broad application of these tools, e.g. in pharmaceutical substance screening, requires neuronal cells that provide stable activity on MEA...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(03)00186-9

    authors: Otto F,Görtz P,Fleischer W,Siebler M

    更新日期:2003-09-30 00:00:00

  • Vibrating glass stylets: tools for precise microsurgery on cuticular structures.

    abstract::Precisely localized lesions in cuticular structures of insects can be produced by fine glass rods, vibrating transversally at frequencies above 100 kHz. The oscillating edge of the glass rod scrapes off the chitinous material at minimal elastic deformation during treatment. The achieved roughness height is in the 0.1 ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(89)90111-8

    authors: Gödde J

    更新日期:1989-07-01 00:00:00

  • In vivo visualization of callosal pathways: a novel approach to the study of cortical organization.

    abstract::I describe here the successful visualization of interhemispheric callosal connections in the live mammalian cortex. The development of this method was prompted by the finding that fluorescent tracer labeling of groups of cortical neurons, when done under optimal conditions, is sufficiently intense to be visible even i...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(88)90137-9

    authors: Malach R

    更新日期:1988-10-01 00:00:00

  • Gaze control in cats achieved by rewarding tongue push movements during the flickering of a light-emitting diode.

    abstract::A method is described for achieving gaze control in cats, by rewarding them for pushing against a lever with their tongue, only when a light-emitting diode (LED) was flickering. Since the state of the LED was changed sequentially from OFF to ON to flickering and the duration of flickering was short, the cats were requ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(80)90036-9

    authors: Hamada T

    更新日期:1980-10-01 00:00:00

  • Design of a fused phantom for quantitative evaluation of brain metabolites and enhanced quality assurance testing for magnetic resonance imaging and spectroscopy.

    abstract:BACKGROUND:Magnetic resonance imaging and spectroscopy (MRI-MRS) is a useful tool for the identification and evaluation of chemical changes in anatomical regions. Quality assurance (QA) is performed in either images or spectra using QA phantom. Therefore, consistent and uniform technical MRI-MRS QA is crucial. NEW MET...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2015.08.005

    authors: Song KH,Kim SY,Lee DW,Jung JY,Lee JH,Baek HM,Choe BY

    更新日期:2015-11-30 00:00:00

  • Interactive 3-dimensional segmentation of MRI data in personal computer environment.

    abstract::We describe a method of interactive three-dimensional segmentation and visualization for anatomical magnetic resonance imaging (MRI) data in a personal computer environment. The visual feedback necessary during 3-D segmentation was provided by a ray casting algorithm, which was designed to allow users to interactively...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(01)00470-8

    authors: Yoo SS,Lee CU,Choi BG,Saiviroonporn P

    更新日期:2001-11-15 00:00:00

  • A charge-metering method for voltage-mode neural stimulation.

    abstract::Electrical neural stimulation is the technique used to modulate neural activity by inducing an instantaneous charge imbalance. This is typically achieved by injecting a constant current and controlling the stimulation time. However, constant voltage stimulation is found to be more energy-efficient although it is chall...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.11.028

    authors: Luan S,Constandinou TG

    更新日期:2014-03-15 00:00:00

  • GAIN: A graphical method to automatically analyze individual neurite outgrowth.

    abstract:BACKGROUND:Neurite outgrowth is a metric widely used to assess the success of in vitro neural stem cell differentiation or neuron reprogramming protocols and to evaluate high-content screening assays for neural regenerative drug discovery. However, neurite measurements are tedious to perform manually, and there is a pa...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2017.03.013

    authors: Long BL,Li H,Mahadevan A,Tang T,Balotin K,Grandel N,Soto J,Wong SY,Abrego A,Li S,Qutub AA

    更新日期:2017-05-01 00:00:00

  • In vivo chronoamperometric measurements of the clearance of exogenously applied serotonin in the rat dentate gyrus.

    abstract::The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of th...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(97)00144-1

    authors: Daws LC,Toney GM,Davis DJ,Gerhardt GA,Frazer A

    更新日期:1997-12-30 00:00:00

  • Modeling the dopaminergic nerve terminal.

    abstract::A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(88)90045-3

    authors: Justice JB Jr,Nicolaysen LC,Michael AC

    更新日期:1988-01-01 00:00:00

  • Flavopiridol reduces the impedance of neural prostheses in vivo without affecting recording quality.

    abstract::We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2009.06.026

    authors: Purcell EK,Thompson DE,Ludwig KA,Kipke DR

    更新日期:2009-10-15 00:00:00

  • In vivo selective monitoring of basal levels of cerebral dopamine using voltammetry with Nafion modified (NA-CRO) carbon fibre micro-electrodes.

    abstract::The electrochemical technique of differential pulse voltammetry (DPV) with micro-biosensors has been used for a number of years to monitor in vivo and in situ changes in the extracellular concentration of cerebral ascorbic acid, as well as that of the metabolites of dopamine (DA) and serotonin (5-HT). We have recently...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(92)90094-t

    authors: Crespi F,Möbius C

    更新日期:1992-05-01 00:00:00

  • In vivo magnetic resonance tracking of Feridex-labeled bone marrow-derived neural stem cells after autologous transplantation in rhesus monkey.

    abstract::Bone marrow stroma cells-derived neural stem cells (BMSCs-D-NSCs) transplantation is a promising strategy for the treatment of nervous system disorders. The development of a non-invasive method to follow the fate of BMSCs-D-NSCs in vivo is very important for the future application of this treatment. In this paper, we ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2009.01.007

    authors: Ke YQ,Hu CC,Jiang XD,Yang ZJ,Zhang HW,Ji HM,Zhou LY,Cai YQ,Qin LS,Xu RX

    更新日期:2009-04-30 00:00:00

  • Acute in vivo testing of a polymer cuff electrode with integrated microfluidic channels for stimulation, recording, and drug delivery on rat sciatic nerve.

    abstract:BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108634

    authors: Elyahoodayan S,Larson C,Cobo AM,Meng E,Song D

    更新日期:2020-04-15 00:00:00

  • Design, construction, and validation of an MRI-compatible vibrotactile stimulator intended for clinical use.

    abstract::Vibrotactile stimulation has been used successfully to activate the human somatosensory pathway in functional magnetic resonance imaging (fMRI) experiments. The design and characterization of these devices are of particular interest in frequency discrimination tasks and investigations of the somatopic organization of ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2009.07.018

    authors: Chakravarty MM,Broadbent S,Rosa-Neto P,Lambert CM,Collins DL

    更新日期:2009-10-30 00:00:00

  • A modification of the Harper-McGinty microdrive for use in chronically prepared rabbits.

    abstract::We describe a modification of the Harper-McGinty microdrive for use with extracellular, microwire recording in conscious, chronically prepared rabbits. Improvements over existing designs are in ease of assembly and mechanical stability. The microdrive is easily fabricated with hand tools and readily available parts. I...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2005.10.020

    authors: McKown MD,Schadt JC

    更新日期:2006-06-15 00:00:00

  • Double diffusion encoding and applications for biomedical imaging.

    abstract::Diffusion Magnetic Resonance Imaging (dMRI) is one of the most important contemporary non-invasive modalities for probing tissue structure at the microscopic scale. The majority of dMRI techniques employ standard single diffusion encoding (SDE) measurements, covering different sequence parameter ranges depending on th...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108989

    authors: Henriques RN,Palombo M,Jespersen SN,Shemesh N,Lundell H,Ianuş A

    更新日期:2021-01-15 00:00:00

  • Efficient gene transfers into neocortical neurons connected by NMDA NR1-containing synapses.

    abstract:BACKGROUND:Within a circuit, specific neurons and synapses are hypothesized to have essential roles in circuit physiology and learning, and dysfunction in these neurons and synapses causes specific disorders. These critical neurons and synapses are embedded in complex circuits containing many neuron and synapse types. ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2019.108390

    authors: Nagayach A,Singh A,Geller AI

    更新日期:2019-11-01 00:00:00

  • Myelin detection in fluorescence microscopy images using machine learning.

    abstract:BACKGROUND:The myelin sheath produced by glial cells insulates the axons, and supports the function of the nervous system. Myelin sheath degeneration causes neurodegenerative disorders, such as multiple sclerosis (MS). There are no therapies for MS that promote remyelination. Drug discovery frequently involves screenin...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108946

    authors: Çimen Yetiş S,Çapar A,Ekinci DA,Ayten UE,Kerman BE,Töreyin BU

    更新日期:2020-12-01 00:00:00

  • Synaptic plasticity in the hippocampal slice: functional consequences.

    abstract::There are 3 known forms of synaptic plasticity at CNS synapses: long-term potentiation (LTP) mediated by NMDA receptor activation, LTP mediated by voltage-dependent calcium channel (VDCC) activation, and long-term depression (LTD) mediated by the NMDA receptor. All 3 forms of synaptic plasticity can be observed in hip...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(94)00188-m

    authors: Teyler TJ,Cavus I,Coussens C

    更新日期:1995-06-01 00:00:00

  • Automatic seizure detection in EEG using logistic regression and artificial neural network.

    abstract::The detection of epileptiform discharges in the EEG is an important component in the diagnosis of epilepsy. In this study, multiple signal classification (MUSIC), autoregressive (AR) and periodogram methods were used to get power spectra in patients with absence seizure. The EEG power spectra were used as an input to ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2005.04.009

    authors: Alkan A,Koklukaya E,Subasi A

    更新日期:2005-10-30 00:00:00

  • Data-driven approach to the estimation of connectivity and time delays in the coupling of interacting neuronal subsystems.

    abstract::One of the challenges in neuroscience is the detection of directionality between signals reflecting neural activity. To reveal the directionality of coupling and time delays between interacting multi-scale signals, we use a combination of a data-driven technique called empirical mode decomposition (EMD) and partial di...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2010.06.004

    authors: Silchenko AN,Adamchic I,Pawelczyk N,Hauptmann C,Maarouf M,Sturm V,Tass PA

    更新日期:2010-08-15 00:00:00

  • AutoSholl allows for automation of Sholl analysis independent of user tracing.

    abstract:BACKGROUND:Sholl analysis has been used to analyze neuronal morphometry and dendritic branching and complexity for many years. While the process has become semi-automated in recent years, existing software packages are still dependent on user tracing and hence are subject to observer bias, variability, and increased us...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2019.108529

    authors: Srinivasan A,Srinivasan A,Ferland RJ

    更新日期:2020-02-01 00:00:00

  • Magnetic resonance angiography of carotid and cerebral arterial occlusion in rats using a clinical scanner.

    abstract::In rat models to induce both focal cerebral ischemia and chronic cerebral hypoperfusion, it is highly desirable to verify the success of vessel occlusion and reopening with non-invasive method. The contrast-agent free 3D time-of-flight magnetic resonance angiography (TOF-MRA), diffusion-weighted imaging (DWI) and T2-w...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2007.08.005

    authors: Yang YM,Feng X,Yao ZW,Tang WJ,Liu HQ,Zhang L

    更新日期:2008-01-30 00:00:00

  • Modification of a consumer digital audio tape (DAT) for analog data recording.

    abstract::A modification of a commercially available digital-audio tape (DAT) for DC recordings such as those used in patch clamp and other electrophysiological experiments is introduced. The modified DAT can record data from 2 channels plus a separate trigger signal for up to 2 h. The bandwidth ranges from direct current to 21...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(92)90071-k

    authors: Lüscher C,Frei P,de Limoges D

    更新日期:1992-12-01 00:00:00