Abstract：
:This work demonstrates technical approaches to high-quality magnetic resonance imaging (MRI) of small structures of the mouse brain in vivo. It turns out that excellent soft-tissue contrast requires the reduction of partial volume effects by using 3D MRI at high (isotropic) resolution with linear voxel dimensions of about 100-150 microm. The long T(2)* relaxation times at relatively low magnetic fields (2.35 T) offer the benefit of a small receiver bandwidth (increased signal-to-noise) at a moderate echo time which together with the small voxel size avoids visual susceptibility artifacts. For measuring times of 1-1.5 h both T(1)-weighted (FLASH) and T(2)-weighted (Fast Spin-Echo) 3D MRI acquisitions exhibit detailed anatomical insights in accordance with histological sections from a mouse brain atlas. Preliminary applications address the identification of neuroanatomical variations in different mouse strains and the use of Mn(2+) as a T(1) contrast agent for neuroaxonal tracing of fiber tracts within the mouse visual pathway.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Natt O,Watanabe T,Boretius S,Radulovic J,Frahm J,Michaelis Tdoi
10.1016/s0165-0270(02)00211-xsubject
Has Abstractpub_date
2002-10-30 00:00:00pages
203-9issue
2eissn
0165-0270issn
1872-678Xpii
S016502700200211Xjournal_volume
120pub_type
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