Abstract:
:Superpositioning of multiple 3-dimensional digital volumes is essential to extending neuroimaging capabilities from single-subject analysis to group evaluation. In this study, 6 rat heads were cryoplaned and digital images of the specimen blockface were organized into data volumes. The midline plane of each brain guided coronal and horizontal rotations and medial-lateral positioning. Four different methods of determining sagittal rotation, dorsoventral position, and anteroposterior position were tested. The first technique uses lambda and bregma as fiducial points; the second method employs bony sutures in the base of the skull; and the third strategy fits both sets of alignments points. The fourth method differs from the previous landmark-based approaches by employing cross-correlation of image densities. A composite of midsagittal cutplanes through the volumes aligned by the first method revealed significant misalignment. Use of ventral fiducials resulted in improved correlation statistics for orthogonal cutplanes through the volumes and significantly reduced positional variability of anteroposterior landmarks. Combining dorsal and ventral fiducials produced a slight improvement in midsagittal image correlation but had mixed effects on landmark variability. Following the fourth method of registration, correlation statistics and the alignment of reference points were similar to those obtained with the 4-point approach.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Santori EM,Toga AWdoi
10.1016/0165-0270(93)90007-esubject
Has Abstractpub_date
1993-11-01 00:00:00pages
187-96issue
2eissn
0165-0270issn
1872-678Xpii
0165-0270(93)90007-Ejournal_volume
50pub_type
杂志文章abstract:BACKGROUND:Manual measurement of three routine parameters of axon regeneration and myelination, such as axon counts, axon caliber and G-Ratio, is tedious and time consuming. An automated or semi-automated computer program could improve the efficiency of this process. The concern, however, is that the automated method w...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.05.032
更新日期:2014-08-15 00:00:00
abstract::The FD Rapid GolgiStain Kit (FD NeuroTechnologies, Inc., Ellicott City, MD) was developed using an improved Golgi-Cox impregnation method to provide stable, sensitive, and convenient staining of mature neurons. However, embryonic neurons cannot be clearly visualized using this method. We have further improved the Golg...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.06.007
更新日期:2012-07-30 00:00:00
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journal_title:Journal of neuroscience methods
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abstract::Preferential exploration of novel locations and objects by rodents has been used to test the effects of various manipulations on object recognition memory. However, manual scoring is time-consuming, requires extensive training, and is subject to inter-observer variability. Since rodents explore primarily by sniffing, ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.11.002
更新日期:2008-03-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.002
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abstract::An encephalomyosynangiosis (EMS) is a temporal muscle graft that is placed onto the surface of the brain to serve as a source for collateral vessel growth for brain revascularization in patients with Moyamoya Disease (MMD). To facilitate an EMS in patients with occlusive cerebrovascular diseases other than MMD, the tr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2011-09-30 00:00:00
abstract::In the method of in situ hybridization autoradiography, quantitative comparisons among multiple mRNA signals have proven difficult for many reasons, attributable both to technical factors (e.g. different probe specific activities) as well as to large differences in the patterns and levels of expression of different ge...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00015-1
更新日期:1999-04-01 00:00:00
abstract::Measurements of neurotransmitters in conscious animals have been restrictive in real-time. The use of specific enzymes within an amperometric probe, based upon a microdialysis membrane, may overcome some of these problems. I report the use of such a probe, with different perfusions of enzymes, to allow real-time measu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(96)02175-9
更新日期:1997-04-04 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.03.024
更新日期:2010-06-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2000-07-31 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00277-6
更新日期:2000-10-15 00:00:00
abstract::Hydraulically coupled volume microejection (HCVM) was employed to overcome known drawbacks of conventional pressure ejection of drugs in isolated brain slices and other tissues. For HCVM, a solution-filled glass micropipette is connected to a motor-driven, liquid-filled syringe by low-compliance, liquid-filled tubing,...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.01.007
更新日期:2006-09-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2009-06-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90047-6
更新日期:1986-04-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00300-9
更新日期:2000-11-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90024-6
更新日期:1988-02-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90046-5
更新日期:1988-01-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108628
更新日期:2020-04-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90064-x
更新日期:1993-03-01 00:00:00
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1998-06-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1980-06-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.04.002
更新日期:2007-08-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.11.016
更新日期:2010-02-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90009-9
更新日期:1986-03-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.019
更新日期:2006-02-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90007-6
更新日期:1988-04-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00169-h
更新日期:1995-05-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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更新日期:2020-10-01 00:00:00