Abstract:
:1321N1 astrocytoma cells are frequently used to analyze stimulus-induced intracellular signaling. These experiments require genetic manipulation of the cells and several chemical and physical methods have been employed in the past. Recently, microporation has been suggested as the best method to transfect 1321N1 astrocytoma cells. Here, we demonstrate that lentiviral gene transfer into 1321N1 cells is highly efficient, cheap and non-toxic. In addition, lentiviral gene transfer efficiently facilitates stable expression of small hairpin RNAs. Finally, lentiviral gene transfer can be used to implant promoter/luciferase reporter genes into the chromatin of the cells, allowing promoter studies using templates that are embedded into the nucleosomal structure of the chromatin.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Keim A,Müller I,Thiel Gdoi
10.1016/j.jneumeth.2012.02.016subject
Has Abstractpub_date
2012-01-01 00:00:00pages
138-42issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(12)00061-1journal_volume
206pub_type
杂志文章abstract:BACKGROUND:Deviant stimuli within a standard, frequent stimulus train induce a cortical somatosensory mismatch response (SMMR). The SMMR reflects the brain's automatic mechanism for the detection of change in a somatosensory domain. It is usually elicited by electrical stimulation, which activates nerve fibers and rece...
journal_title:Journal of neuroscience methods
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abstract::The neocortex is the site of origin of several forms of acquired epilepsy. Here we provide a brief review of experimental models that were recently developed to study neocortical epileptogenesis as well as some major results obtained with these methods. Most of neocortical seizures appear to be nocturnal and it is kno...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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abstract::A method for the simultaneous visualization of neurotransmitter receptor binding sites and immunohistochemically characterized cells is described using in vitro sections of rat striatum. The striatum provides a rich neurochemical environment in which to examine muscarinic acetylcholine receptor relations to peptide-re...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90061-5
更新日期:1985-10-01 00:00:00
abstract::Knowledge of the precise position of recording microelectrodes within the brain of a non-human primate is essential for a reliable exploration of very small anatomic structures. This work demonstrates the compatibility of a newly developed glass-guided microelectrode design and microfeed equipment with high-resolution...
journal_title:Journal of neuroscience methods
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abstract::A new video path analyzer, described herein, monitors travel distance, rearing, stereotypic movement, rotation, and speed of rats simultaneously. The video path analyzer was equipped with a cage (black background, 50 cm long x 50 cm wide x 50 cm high), camera, television screen, computer, and printer. A detailed descr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00101-X
更新日期:1996-12-01 00:00:00
abstract::Motorized linear slides used as micromanipulators for biological use suffer from 3 problems: vibration at low speed, poor ergonomic design of the controller and slow coarse positioning. These problems are not hard to solve by minor modifications to the controller and the slides. ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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更新日期:2015-01-30 00:00:00
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2014-01-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(91)90041-w
更新日期:1991-02-01 00:00:00
abstract::It has become increasingly evident that continuous EEG monitoring is necessary to observe the development of epilepsy in animals, and to determine the effect of drugs on spontaneous seizures. Telemetric recording systems have been increasingly used to monitor EEG in freely moving animals. One challenge faced by such s...
journal_title:Journal of neuroscience methods
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更新日期:2011-09-30 00:00:00
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journal_title:Journal of neuroscience methods
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更新日期:2018-07-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90094-v
更新日期:1990-10-01 00:00:00
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journal_title:Journal of neuroscience methods
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更新日期:2006-09-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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abstract:BACKGROUND:Zebrafish larvae have a high potential as model system to replace rodents, especially in screening and drug discovery applications. However, an experimental setup to deliver mild electrical stimuli with simultaneous high throughput behavioural tracking has not yet been described. NEW METHOD:A new tool was d...
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abstract::Previous research has shown that spatio-temporal waves in the EEG are generally of long spatial wavelength and form smooth patterns of phase gradients at particular time-samples. This paper describes a method to measure smooth phase gradients of long spatial wavelength in the EEG. The method depends on the global patt...
journal_title:Journal of neuroscience methods
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