Abstract:
:Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verification of a procedure which selectively stimulates pulpal nerves over a wide range of stimulus intensities. The methods consisted of placing electrodes in labial cavities of each of the two lower rat incisors, 1 mm distal to the level of the crest of the interdental papilla. Each cavity had a diameter of 0.5 mm and a depth of about three-quarters fo the crown diameter. Each electrode consisted of a Ag/AgCl filling compacted onto the end of a 0.1 mm silver wire. Constant current stimuli were passed between the two electrodes. EMG was monitored to detect reflex activation of the digastric muscle in response to intradental or extradental afferent excitation. The response consisted of two components. One was a long latency potential (LLP) (37 ms latency) (89 microA ave TH); the other was a short latency potential (SLP) (12 ms latency) (650 microA min TH). In all animals that LLP was abolished by bilateral sectioning of the tooth pulp 7 mm below the level of the labial gingival margin. The SLP was not abolished. This would indicate that the LLP was due to stimulation of pulpal fibers, and the SLP to excitation of extrapulpal fibers. This may prove to be a helpful method for nociceptive researchers to easily verify that they are actually stimulating pulpal afferents.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Myslinski N,Matthews Bdoi
10.1016/0165-0270(87)90092-6subject
Has Abstractpub_date
1987-11-01 00:00:00pages
73-8issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(87)90092-6journal_volume
22pub_type
杂志文章abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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abstract::I describe here the successful visualization of interhemispheric callosal connections in the live mammalian cortex. The development of this method was prompted by the finding that fluorescent tracer labeling of groups of cortical neurons, when done under optimal conditions, is sufficiently intense to be visible even i...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90137-9
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abstract:BACKGROUND:Currently the problem of incorporating priori information into an independent component analysis (ICA) model is often solved under the framework of constrained ICA, which utilizes the priori information as a reference signal to form a constraint condition and then introduce it into classical ICA. However, it...
journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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abstract:BACKGROUND:Fluorescence membrane markers are efficient tools for visualizing the dynamics of membrane recycling processes in living cells. The outer hair cell (OHC) - a bipolar epithelial cell in the cochlea - possesses endocytic activity at both its apical and basal poles. The best visual overview of transcytosis in t...
journal_title:Journal of neuroscience methods
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abstract::The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1997-06-06 00:00:00
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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更新日期:1992-05-01 00:00:00
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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更新日期:2012-09-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90056-8
更新日期:1988-06-01 00:00:00
abstract::A new neuroanatomical method based on the anterograde transport of biocytin has recently been reported. The validity of this method is examined closely on a number of well established pathways in the central nervous system, as well as the experimental parameters necessary for its effective use. iontophoretic applicati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(91)90041-w
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abstract::Duchenne muscular dystrophy (DMD) is an X-chromosome-linked disorder that arises from a mutation in the gene for the cytoskeletal protein dystrophin, normally expressed in the myofibres. The most widely applied animal model in DMD basic research is the C57BL/10ScSn-mdx/J mouse, commonly referred to as the "mdx mouse"....
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2019-05-15 00:00:00