Abstract：
:We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We correlate the progressive loss of resting membrane potential and the progressive inability to generate stimulated action potentials by cultured mouse dorsal root ganglion neurons with increasing incorporation of PI. The scoring system used to gauge incorporation of PI is rapid and highly reproducible using a standard fluorescence microscope. Applications exist for studies of neuronal toxicity, survival, and electrophysiology in vitro.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Macklis JD,Madison RDdoi
10.1016/0165-0270(90)90007-3subject
Has Abstractpub_date
1990-01-01 00:00:00pages
43-6issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(90)90007-3journal_volume
31pub_type
杂志文章abstract：:Scanning ion conductance microscopy (SICM) takes advantage of the increase in the resistance which occurs if a glass microelectrode is closely approached to a poorly conducting membrane (Science 243 (1989) 641) and has been shown to be a promising technique to study membranes of living cells (Biophys J 73 (1997a) 653;...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00023-7
更新日期：2002-05-15 00:00:00
abstract：:A modification of a commercially available digital-audio tape (DAT) for DC recordings such as those used in patch clamp and other electrophysiological experiments is introduced. The modified DAT can record data from 2 channels plus a separate trigger signal for up to 2 h. The bandwidth ranges from direct current to 21...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90071-k
更新日期：1992-12-01 00:00:00
abstract：:A method is described for the production of an electrode capable of monitoring and modulating the input-output relationship of thalamic neurons. Tungsten-in-glass electrodes were manufactured with the ability to simultaneously record lateral geniculate nucleus action potentials and associated, retinally evoked S-poten...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90126-c
更新日期：1993-09-01 00:00:00
abstract：:The generation and presentation of tactile stimuli presents a unique challenge. Unlike vision and audition, in which standard equipment such as monitors and audio systems can be used for most experiments, tactile stimuli and/or stimulators often have to be tailor-made for a given study. Here, we present a novel tactil...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.10.012
更新日期：2007-03-30 00:00:00
abstract：:A newly designed combination microelectrode-injection system is described which can be used to record electrophysiological responses of individual neurons in the primate brain to local administration of small quantities of drugs or other compounds. The assembly of the system is simple, and the materials used are inexp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.03.015
更新日期：2004-09-30 00:00:00
abstract：:A modification of the formalin test appropriate for testing of mice is described. Formalin 1 or 5% was injected into the dorsal surface of a hindpaw, and the time the animal spent licking the paw was recorded. On the basis of the response pattern, two distinct periods of intensive licking activity were identified; an ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90116-5
更新日期：1985-06-01 00:00:00
abstract：:We investigate the mode locking properties of simple dynamical models of pulse-coupled neurons to two tones, i.e., simple musical intervals. A recently proposed nonlinear synchronization theory of musical consonance links the subjective ranking from consonant to dissonant intervals to the universal ordering of robustn...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.041
更新日期：2009-09-30 00:00:00
abstract：BACKGROUND:Functional magnetic resonance imaging is well suited to link neural population activation with movement parameters of complex natural arm movements. However, currently existing MR-compatible arm tracking devices are not constructed to measure arm joint movement parameters of unrestricted movements. Therefore...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.01.015
更新日期：2017-03-15 00:00:00
abstract：:A sensitive, enzymatic glucose electrode was coupled with the microdialysis sampling technique to enable the continuous, on-line measurement of dialysate glucose. The glucose sensitive electrode was fabricated by immobilizing glucose oxidase onto the surface of an osmium-polyvinylpyrridine horse radish peroxidase gel ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00119-2
更新日期：1997-12-01 00:00:00
abstract：:A program, written in the language Pascal, is described for signal averaging with a microcomputer. The program instructs the computer to acquire data from two channels on-line. For up to 3 channels of extracellular or intracellular recording analysed simultaneously, it was found not to be necessary to employ machine c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90006-8
更新日期：1985-01-01 00:00:00
abstract：:Automatic analysis of locomotion in studies of behavior and development is of great importance because it eliminates the subjective influence of evaluators on the study. This study aimed to develop and test the reproducibility of a system for automated analysis of locomotor activity in rats. For this study, 15 male Wi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.016
更新日期：2011-02-15 00:00:00
abstract：BACKGROUND:The application of single-cell RNA sequencing (scRNASeq) represents a unique approach to identify hundreds to millions of cells in mammalian cortical multilayers at different stages of embryogenesis. ScRNASeq technology applied to neurological studies requires the use of fresh starting materials because stan...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108960
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND:Current assessment of sport-related concussion involves a multifaceted approach including neurocognitive testing, symptom report, and balance assessment. Lacking from this paradigm is a truly objective measure of the physiological changes associated with concussion. NEW METHOD:A device called the Brain Acou...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.003
更新日期：2014-01-15 00:00:00
abstract：:A microthermistor positioned close to the exposed posterior semicircular canal in isolated labyrinth preparations of the frog was used to stimulate the sensory organ. Our results indicated that, depending on the position of the heater, the induced endolymphatic convection currents may result in either excitatory or in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00021-7
更新日期：1999-05-01 00:00:00
abstract：BACKGROUND:While several novel therapeutic approaches for HD are in development, resources to conduct clinical trials are limited. Large animal models have been proposed to improve assessment of safety, tolerability and especially to increase translational reliability of efficacy signals obtained in preclinical studies...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.11.013
更新日期：2016-05-30 00:00:00
abstract：:In recent years, various applications of brain computer interfaces (BCIs) have been studied. In this paper, we present a hybrid BCI combining P300 and motor imagery to operate an explorer. Our system is mainly composed of a BCI mouse, a BCI speller and an explorer. Through this system, the user can access his computer...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.06.015
更新日期：2015-04-15 00:00:00
abstract：:Stress has long been known to affect grooming in rodent species, altering both its activity measures and behavioural microstructure. Since stress disturbs a general pattern of self-grooming uninterrupted cephalocaudal progression, the grooming analysis algorithm (Kalueff and Tuohimaa, Brain Res. Protocols, 2004; 15: 1...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.10.001
更新日期：2005-04-30 00:00:00
abstract：:The 2-deoxyglucose (2DG) histochemical method of Sokoloff et al. ((1977) J. Neurochem., 28: 897-916) has been widely used to map metabolic activity in the brain, but lacks sufficient resolution to view individual neurons. Modifications of the original method have achieved cellular resolution with emulsion autoradiogra...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00063-5
更新日期：1996-09-01 00:00:00
abstract：BACKGROUND:Contralateral seventh cervical nerve transfer (contralateral C7 transfer) is a novel treatment for patients with spastic paralysis, including stroke and traumatic brain injury. However, little is known on changes in plasticity that occur in the intact hemisphere after C7 transfer. An appropriate surgical mod...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108445
更新日期：2019-12-01 00:00:00
abstract：BACKGROUND:Steady-state visual-evoked potential (SSVEP)-based brain-computer interfaces (BCIs) generate weak SSVEP with a monitor and cannot use harmonic frequencies, whereas P300-based BCIs need multiple stimulation sequences. These issues can decrease the information transfer rate (ITR). NEW METHOD:In this paper, we...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.11.001
更新日期：2016-01-30 00:00:00
abstract：BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108903
更新日期：2020-11-01 00:00:00
abstract：:Diffusion-weighted MRI is the forerunner of the rapidly developed microstructure MRI (μMRI) aimed at in vivo evaluation of the cellular tissue architecture. This brief review focuses on the spatiotemporal scales of the microstructure that are accessible using different diffusion MRI techniques and the need to weight t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2020.108910
更新日期：2021-01-01 00:00:00
abstract：:The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90021-9
更新日期：1984-04-01 00:00:00
abstract：:A method by which muscles, nerves, and a section of spinal cord from a turtle can be stably maintained in vitro for many days has been developed. Tests of the viability of central and neuromuscular synapses, and the histochemical properties of muscle fibers have indicated that the preparation is viable and shows littl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90029-0
更新日期：1989-08-01 00:00:00
abstract：:Previous research has shown that spatio-temporal waves in the EEG are generally of long spatial wavelength and form smooth patterns of phase gradients at particular time-samples. This paper describes a method to measure smooth phase gradients of long spatial wavelength in the EEG. The method depends on the global patt...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.02.016
更新日期：2006-09-30 00:00:00
abstract：BACKGROUND:Photothrombosis is a minimally invasive method for induction of cortical ischemia. However, different ways of applying some methods to assess photothrombosis-induced damage need to be developed. NEW METHODS:We applied the tongue protrusion test and H&E staining of brain sections to detect ischemic damage af...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108457
更新日期：2020-01-01 00:00:00
abstract：:Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.07.007
更新日期：2009-09-30 00:00:00
abstract：:An improved method using HRP for visualizing axons in whole-mount preparations is described. Application of this method to preparations of regenerating frog peripheral nerves maintained in organ culture was used to show that regeneration following nerve crush begins after about 3 days. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90110-y
更新日期：1992-08-01 00:00:00
abstract：BACKGROUND:Large-scale Granger causality (lsGC) is a recently developed, resting-state functional MRI (fMRI) connectivity analysis approach that estimates multivariate voxel-resolution connectivity. Unlike most commonly used multivariate approaches, which establish coarse-resolution connectivity by aggregating voxel ti...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.06.007
更新日期：2017-08-01 00:00:00
abstract：:The present study describes a method for improving the precision and accuracy of clot placement within the middle cerebral artery (MCA) of rats, utilizing a micro-catheter and laser Doppler flowmetry. This technique reduces the size of clot needed to achieve stable occlusion with no failed embolizations and a low perc...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.12.026
更新日期：2006-06-30 00:00:00
