Abstract:
:Stress has long been known to affect grooming in rodent species, altering both its activity measures and behavioural microstructure. Since stress disturbs a general pattern of self-grooming uninterrupted cephalocaudal progression, the grooming analysis algorithm (Kalueff and Tuohimaa, Brain Res. Protocols, 2004; 15: 151-8) was previously designed for mice to enable the detection of stress by measuring alterations in grooming microstructure in different test situations. Since mice and rats are known to differ in their behaviours, including grooming, the aim of the current study was to test our approach in rats and evaluate the utility of this method for differentiation between high- and low-stress situations. For this, we have developed the rat grooming analysis algorithm (based on ethological analysis of incorrect transitions contrary to the cephalocaudal rule, interrupted grooming activity and the assessment of the regional distribution of grooming) and applied this algorithm to the light-exposed (high stress) and dark-exposed (low stress) groups of rats. Here, we show that the percentage of 'incorrect' transitions between different grooming patterns, the percentage of interrupted grooming bouts and altered regional distribution of grooming (less caudal grooming, more rostral grooming) may be used as behavioural markers of stress in rats. Our results suggest that this method can be a useful tool in neurobehavioural stress research including modelling stress-evoked states, psychopharmacological or behavioural neurogenetics research in rats.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kalueff AV,Tuohimaa Pdoi
10.1016/j.jneumeth.2004.10.001subject
Has Abstractpub_date
2005-04-30 00:00:00pages
169-77issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(04)00362-0journal_volume
143pub_type
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