Abstract:
:A sensitive, enzymatic glucose electrode was coupled with the microdialysis sampling technique to enable the continuous, on-line measurement of dialysate glucose. The glucose sensitive electrode was fabricated by immobilizing glucose oxidase onto the surface of an osmium-polyvinylpyrridine horse radish peroxidase gel (Os-gel-HRP) which had been cast coated onto a glassy carbon electrode. This 'bilayer' electrode generated a reductive current to glucose at a potential of 0 mV thereby minimizing faradic oxidative interferences. The system utilized the continuous mixing of two fluids immediately prior to the 'bilayer' electrode. One fluid was the dialysate. The other was an oxygenated, low pH phosphate buffer which minimized oxidative interference, buffered the electrode from variations of pH and maximized enzyme efficiency. In practical terms, the 'bilayer' electrode was simple to manufacture, quick to reach stable basal currents (less than 60 min), sensitive (2.5 microM glucose could be detected in the dialysate) and durable (usable for up to 3 days). In vivo experiments, used the smallest commercially available microdialysis probes to demonstrate that on-line, continuous measurements of EC striatal glucose in the dialysate were receptive to pharmacological (local perfusion with veratridine (50 microM), systemic hyperglycemia (1.5 ml of 0.55 M glucose intraperitoneal (i.p.)) and anesthesia (Nembutal 40 mg/kg i.p.)) and behavioral (restraint) manipulations. This technique allows for greater temporal resolution than conventional HPLC procedures whilst requiring significantly less technical outlay or analytical expertise. The high sensitivity of the analytical technique could facilitate the study of EC glucose levels in very localized regions of the brain if coupled to microdialysis probes of small dimensions.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Osborne PG,Niwa O,Kato T,Yamamoto Kdoi
10.1016/s0165-0270(97)00119-2subject
Has Abstractpub_date
1997-12-01 00:00:00pages
143-50issue
2eissn
0165-0270issn
1872-678Xpii
S0165027097001192journal_volume
77pub_type
杂志文章abstract::The force-plate actometer is a relatively new computer-based instrument with high temporal and spatial resolution that has been used to measure the behavioral effects of genetic restriction (e.g., inbred mice) and drugs (e.g., dopaminergic agonists and antagonists) on a variety of behaviors in rodents, including locom...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.10.002
更新日期:2006-05-15 00:00:00
abstract::MicroRNAs (miRNAs) finely tune messenger RNA (mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei. MiRNA expression profiling from individual samples is also important for investigating potential dif...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.05.033
更新日期:2012-07-30 00:00:00
abstract::A non-invasive integrated method was developed to measure neural and behavioural responses to peripheral sensory and noxious stimulation in human infants. The introduction of a novel event-detection interface allows synchronous recording of: (i) muscle and central nervous system activity with surface electromyography ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.01.009
更新日期:2012-04-15 00:00:00
abstract::Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.03.013
更新日期:2009-06-15 00:00:00
abstract::Neuronal loss in tissue surrounding an intracerebral hemorrhage (ICH) is usually quantified by labor-intensive histological methods that are subject to bias. Fluorescent protein expression has been successfully used as a marker of cell viability in vitro and in retinal studies in vivo, but not in any ICH model to date...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.03.025
更新日期:2013-06-15 00:00:00
abstract::A novel version of the 8-arm radial maze task was developed to quantify spatial learning and memory in rats subjected to transient cerebral ischemia (TCI) using the 4-VO model. This maze uses the rat's natural behavior of avoiding open, illuminated areas, and preference for a darkened, enclosed shelter. Ischemic rats ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.08.012
更新日期:2004-01-15 00:00:00
abstract::We present a client-server application for the distributed multivariate analysis of time series using standard PCs. We here concentrate on analyses of multichannel EEG/MEG data, but our method can easily be adapted to other time series. Due to the rapid development of new analysis techniques, the focus in the design o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.002
更新日期:2006-04-15 00:00:00
abstract::An inexpensive system for quantitative electroencephalogram (EEG) analysis over long time periods is described. This system continuously analyses EEG for intensity versus frequency content, and saves averaged 'data reduced' intensity spectra to disk at regular intervals. The method had adequate frequency resolution fo...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90066-o
更新日期:1990-04-01 00:00:00
abstract::Non-invasive neuroimaging is increasingly used for investigating the human amygdala. Accurate functional localization in the amygdala region is, however, challenging and quantitative data on the anatomical specificity of functional amygdala imaging is lacking. We have therefore retrospectively investigated 114 recentl...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.02.022
更新日期:2009-05-30 00:00:00
abstract::We describe a much simplified high-performance liquid chromatography (HPLC) method for the measurement of caffeine in plasma and brain. A particularly attractive feature of this method is that a simple methanol/water (60:40) mobile phase can be used both for plasma and brain samples. In addition, the method is compati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)90139-2
更新日期:1994-07-01 00:00:00
abstract:BACKGROUND:Recording and manipulating neural activity in awake behaving animal models requires long-term implantation of cranial implants that must address a variety of design considerations, which include preventing infection, minimizing tissue damage, mechanical strength of the implant, and MRI compatibility. NEW ME...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.12.011
更新日期:2015-02-15 00:00:00
abstract::Songbirds are the preeminent animal model for understanding how the brain encodes and produces learned vocalizations. Here, we report a new statistical method, the Kullback-Leibler (K-L) distance, for analyzing vocal change over time. First, we use a computerized recording system to capture all song syllables produced...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.033
更新日期:2008-09-15 00:00:00
abstract::The weakly acidic, cation-exchange resin Bio-Rex 70 was used at a low pH for chromatography of enkephalins and other peptides containing enkephalin sequences. Recoveries of various peptide standards and peptides in adrenal chromaffin cell extracts were greater than 85%. Catecholamines and other substances such as dens...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90052-4
更新日期:1985-10-01 00:00:00
abstract::To facilitate the combination of optical imaging with various electrode-based techniques, we have designed and produced a skull-mounting "sliding-top cranial window" and a removable "electrode positioner microdrive". These new devices were used to study sensory processing in chronic and acute experiments in the cerebr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00022-5
更新日期:2002-04-30 00:00:00
abstract::Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90114-8
更新日期:1988-08-01 00:00:00
abstract:BACKGROUND:To optimize survival evaluation of Schwann cells (SCs) in vivo, we tested fluorescent labeling of the nucleus as an improved method of tracking and counting the transplanted SCs at sciatic nerve injury sites in rodents. We also investigated if co-administering cells with the glial growth factor Neuregulin-1 ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.08.009
更新日期:2016-11-01 00:00:00
abstract::Previously, we developed technology that coupled high-speed chronoamperometry with microejections of dopamine (DA) to measure DA clearance in the brains of freely-behaving rats. Here, by varying the ejection volumes of DA across a 200-fold difference, the kinetics of striatal clearance were analyzed as a function of t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00229-7
更新日期:2002-11-15 00:00:00
abstract::A high-speed voltammetric system was designed and tested for dual measurement of dopamine (DA) and serotonin (5-HT) at 250-ms intervals. First, an anodic-cathodic square-wave pulse was delivered to activate the electrode (carbon fiber, 7 microm in diameter), then DA and 5-HT oxidation currents (current intensity) were...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00056-4
更新日期:1999-07-15 00:00:00
abstract::Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not s...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.007
更新日期:2008-08-30 00:00:00
abstract::Three-dimensional (3D) reaggregate tissue culture provides a means of quantitatively assessing neuronal cell survival under a variety of experimental conditions. A method is presented for estimation of, and comparison between, the total numbers of cells of a given neurochemical type within individual experimental flas...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)00110-3
更新日期:1995-01-01 00:00:00
abstract::Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.09.034
更新日期:2009-02-15 00:00:00
abstract::In rat models to induce both focal cerebral ischemia and chronic cerebral hypoperfusion, it is highly desirable to verify the success of vessel occlusion and reopening with non-invasive method. The contrast-agent free 3D time-of-flight magnetic resonance angiography (TOF-MRA), diffusion-weighted imaging (DWI) and T2-w...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.08.005
更新日期:2008-01-30 00:00:00
abstract::Mammalian brain glycogen is adequate to support oxidative metabolism for several minutes. The present studies were done primarily to develop the guinea pig hippocampal slice as a model for studying the function and regulation of that glycogen. Slice glycogen falls to 6 nmol/mg dry wt. during the first hour of incubati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90019-8
更新日期:1989-05-01 00:00:00
abstract:BACKGROUND:Current neuronal monitoring techniques, such as calcium imaging and multi-electrode arrays, enable recordings of spiking activity from hundreds of neurons simultaneously. Of primary importance in systems neuroscience is the identification of cell assemblies: groups of neurons that cooperate in some form with...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.08.011
更新日期:2014-10-30 00:00:00
abstract::Monkeys were partially surrounded by opaque screens except for some distant small holes through which they observed various stimuli or actions, or well-defined background patterns. Gaze direction was measured by an infrared cornea reflection method. Without training or reward, the animal's gaze was directed through on...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.02.024
更新日期:2004-08-30 00:00:00
abstract::Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the rapid degradation of fatty acid amides such as the endocannabinoid anandamide. Inhibition of FAAH activity has been suggested as a therapeutic approach for the treatment of chronic pain, depression and anxiety, through local activation of the cannabin...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.10.006
更新日期:2007-03-30 00:00:00
abstract::We describe a method for in vivo confocal fluorescence imaging of synaptic terminals and subsequent electron microscopic reconstructions of the same terminals. By iontophoretically applying lipophilic dye to nerve terminals at a single neuromuscular junction with a sharp microelectrode in living neonatal mice, we were...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00096-5
更新日期:1999-10-30 00:00:00
abstract::Beamforming offers a way to estimate the solution to the inverse problem in EEG and MEG but is also known to perform poorly in the presence of highly correlated sources, e.g. during binaural auditory stimulation, when both left and right primary auditory cortices are activated simultaneously. Surface Laplacian, or the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.05.001
更新日期:2013-08-15 00:00:00
abstract:BACKGROUND:Automated segmentation of brain structures is an important task in structural and functional image analysis. We developed a fast and accurate method for the striatum segmentation using deep convolutional neural networks (CNN). NEW METHOD:T1 magnetic resonance (MR) images were used for our CNN-based segmenta...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.10.007
更新日期:2016-12-01 00:00:00
abstract::The physical basis for electrical stimulation of excitable tissue, as used by electrophysiological researchers and clinicians in functional electrical stimulation, is presented with emphasis on the fundamental mechanisms of charge injection at the electrode/tissue interface. Faradaic and non-Faradaic charge transfer m...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2004.10.020
更新日期:2005-02-15 00:00:00