A simplified method for the measurement of caffeine in plasma and brain: evidence for a cortical-subcortical caffeine concentration differential in brain.


:We describe a much simplified high-performance liquid chromatography (HPLC) method for the measurement of caffeine in plasma and brain. A particularly attractive feature of this method is that a simple methanol/water (60:40) mobile phase can be used both for plasma and brain samples. In addition, the method is compatible with solid-phase extraction for plasma samples and conventional brain tissue preparation for biogenic amine analysis with HPLC. Using this method to measure the concentrations of caffeine in plasma and brain of rats which received 10 or 50 mg/kg caffeine injections, we found substantial concentration differences between cortical and subcortical brain tissue. Specifically, at the 10 mg/kg dose, a nearly 2-fold difference between cortex and striatum caffeine concentrations was observed. A shortcoming of many neurobehavioral studies of caffeine effects is the absence of caffeine concentration measurements. The simplicity of the present method for the measurement of caffeine in plasma and brain tissue makes it a practical and feasible procedure to incorporate into neurobehavioral studies designed to elucidate the CNS actions of caffeine.


J Neurosci Methods


Carey RJ,DePalma G




Has Abstract


1994-07-01 00:00:00














  • Automated detection of intercellular signaling in astrocyte networks using the converging squares algorithm.

    abstract::Intercellular calcium waves in central nervous system astrocyte networks underline the principle mechanism of cell signaling in astrocyte syncsytiums, which putatively contribute to the modulation of neuronal signaling and metabolic regulation. In support of carrying out systems level analyses of astrocyte networks, w...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hashemi M,Buibas M,Silva GA

    更新日期:2008-05-30 00:00:00

  • Method of analysis of local neuronal circuits in the vertebrate central nervous system.

    abstract::Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Reinis S,Weiss DS,McGaraughty S,Tsoukatos J

    更新日期:1992-06-01 00:00:00

  • VLSI circuits implementing computational models of neocortical circuits.

    abstract::This paper overviews the design and implementation of three neuromorphic integrated circuits developed for the COLAMN ("Novel Computing Architecture for Cognitive Systems based on the Laminar Microcircuitry of the Neocortex") project. The circuits are implemented in a standard 0.35 μm CMOS technology and include spiki...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wijekoon JH,Dudek P

    更新日期:2012-09-15 00:00:00

  • The separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection.

    abstract::There is increasing evidence that oxidative stress plays an important role in the pathogenesis of many neurodegenerative diseases including Parkinson's disease (PD). In particular there is support for the participation of oxidized catecholamines in PD. Catecholamines are highly reactive and are readily oxidized to ami...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ochs SD,Westfall TC,Macarthur H

    更新日期:2005-03-30 00:00:00

  • Behavioral phenotyping of minipigs transgenic for the Huntington gene.

    abstract:BACKGROUND:While several novel therapeutic approaches for HD are in development, resources to conduct clinical trials are limited. Large animal models have been proposed to improve assessment of safety, tolerability and especially to increase translational reliability of efficacy signals obtained in preclinical studies...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Schramke S,Schuldenzucker V,Schubert R,Frank F,Wirsig M,Ott S,Motlik J,Fels M,Kemper N,Hölzner E,Reilmann R

    更新日期:2016-05-30 00:00:00

  • A method for analysis of encoding of stimulus separation in ensembles of afferents.

    abstract::This paper describes a new method for the analysis of ensemble coding in populations of receptor afferents. The method is based on principal component analysis (PCA) combined with algorithms for calculation of stimulus separation (discrimination). It allows a quantification of the ability of ensembles of afferents to ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Johansson H,Bergenheim M,Djupsjöbacka M,Sjölander P

    更新日期:1995-12-01 00:00:00

  • Detecting differences with magnetoencephalography of somatosensory processing after tactile and electrical stimuli.

    abstract:BACKGROUND:Deviant stimuli within a standard, frequent stimulus train induce a cortical somatosensory mismatch response (SMMR). The SMMR reflects the brain's automatic mechanism for the detection of change in a somatosensory domain. It is usually elicited by electrical stimulation, which activates nerve fibers and rece...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hautasaari P,Kujala UM,Tarkka IM

    更新日期:2019-01-01 00:00:00

  • Intracellular injections of permanent tracers in the fixed slice: a comparison of HRP and biocytin.

    abstract::Here we describe a method for intracellularly injecting mixtures of the fluorescent dye Lucifer Yellow and the permanent tracers HRP or biocytin into aldehyde-fixed slices of the dorsal lateral geniculate nucleus in young postnatal cats. Lucifer Yellow was used for visual control in the injection procedure and the inc...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Coleman LA,Friedlander MJ

    更新日期:1992-09-01 00:00:00

  • The image-combining computer microscope--an interactive instrument for morphometry of the nervous system.

    abstract::The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Glaser EM,Tagamets M,McMullen NT,Van der Loos H

    更新日期:1983-05-01 00:00:00

  • The effects of various methods of sacrifice and of ethanol on adenosine levels in selected areas of rat brain.

    abstract::The effect of acute ethanol on adenosine content in four motor areas of the male Sprague-Dawley rat brain was investigated using HPLC-fluorescence detection. Since basal adenosine levels are difficult to assess due to extremely rapid turnover of adenosine, four different methods of sacrifice were also evaluated for ad...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Clark M,Dar MS

    更新日期:1988-10-01 00:00:00

  • Non-contact eye-tracking on cats.

    abstract::The objective of visual systems neuroscience has shifted over the past few years from determining the receptive fields of cells towards the understanding of higher level cognition in awake animals viewing natural stimuli. In experiments with awake animals it is important to control the relevant aspects of behavior. Mo...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Körding KP,Kayser C,Betsch BY,König P

    更新日期:2001-09-30 00:00:00

  • A simple intrapipette salt bridge.

    abstract::A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kleene SJ

    更新日期:1993-01-01 00:00:00

  • New methods of dye application for staining motor neurons in an insect.

    abstract::Motor neurons innervating tiny muscles in the antennal system of the cockroach, Periplaneta americana, were stained by two new approaches to backfilling motor axons and somata. Both methods are easier, and produce less leakage of dye, than conventional methods that rely on application of dye to the distal cut end of a...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Baba Y

    更新日期:2000-06-01 00:00:00

  • Simultaneous, selective detection of catecholaminergic and indolaminergic signals using cyclic voltammetry with treated micro-sensor.

    abstract::Selective and simultaneous voltammetric analysis of catechols and indoles in vivo and in vitro has until now been feasible only by means of 'slow' scanning methods (scan speed in tens of seconds) such as differential pulse (DPV) and differential normal pulse voltammetry in conjunction with electrically and/or chemical...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Crespi F,England TG,Trist DG

    更新日期:1995-09-01 00:00:00

  • MR-guided stereotactic navigation.

    abstract::Functional magnetic resonance imaging allows precise localization of brain regions specialized for different perceptual and higher cognitive functions. However, targeting these deep brain structures for electrophysiology still remains a challenging task. Here, we propose a novel framework for MRI-stereotactic registra...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ohayon S,Tsao DY

    更新日期:2012-03-15 00:00:00

  • Sampling variation caused by A/D cards due to external trigger.

    abstract::In electrophysiological recording, a microcomputer-based analog-to-digital (A/D) card is an indispensable instrument for signal acquisition and analysis. In our studies, evoked responses sampled by our A/D card showed variation among different cycles. If several cycles had been averaged, the resultant waveform would h...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Jaw FS,Lin CW,Wang CY

    更新日期:1995-11-01 00:00:00

  • A simple method for quantifying changes in neuronal populations in primary cultures of dissociated rat brain.

    abstract::A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Tucker LM,Morton AJ

    更新日期:1995-07-01 00:00:00

  • Improving network inference: The impact of false positive and false negative conclusions about the presence or absence of links.

    abstract:BACKGROUND:A reliable inference of networks from data is of key interest in the Neurosciences. Several methods have been suggested in the literature to reliably determine links in a network. To decide about the presence of links, these techniques rely on statistical inference, typically controlling the number of false ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Cecchini G,Thiel M,Schelter B,Sommerlade L

    更新日期:2018-09-01 00:00:00

  • A novel device for studying weight supported, quadrupedal overground locomotion in spinal cord injured rats.

    abstract:BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hamlin M,Traughber T Jr,Reinkensmeyer DJ,de Leon RD

    更新日期:2015-05-15 00:00:00

  • Characterization of rat rostral raphe primary cultures: multiplex quantification of serotonergic markers.

    abstract::Previous reports establishing raphe cultures typically yield less than 1% serotonin (5-HT)-positive neurons and are impractical for transcriptional studies. In this study, we have established primary cultures enriched in 5-HT neurons and quantified the proportion of cells expressing serotonergic and non-serotonergic m...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Czesak M,Burns AM,Remes Lenicov F,Albert PR

    更新日期:2007-08-15 00:00:00

  • Selective, quantitative measurement of releasable synaptic zinc in human autopsy hippocampal brain tissue from Alzheimer's disease patients.

    abstract::Aberrant central nervous system zinc homeostasis has been reported in Alzheimer's disease (AD). However, there are conflicting reports describing zinc concentration either increased or decreased in the brain of AD patients. Such discrepancies may be due to differences in the brain area examined, zinc detection method,...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Bjorklund NL,Sadagoparamanujam VM,Taglialatela G

    更新日期:2012-01-15 00:00:00

  • Extracting multi-mode ERP features using fifth-order nonnegative tensor decomposition.

    abstract:BACKGROUND:Preprocessed Event-related potential (ERP) data are usually organized in multi-way tensor, in which tensor decomposition serves as a powerful tool for data processing. Due to the limitation of computation burden for multi-way data and the low algorithm performance of stability and efficiency, multi-way ERP d...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wang D,Zhu Y,Ristaniemi T,Cong F

    更新日期:2018-10-01 00:00:00

  • High time resolution fluorescence imaging with a CCD camera.

    abstract::We have built a high speed, sensitive camera system capable of capturing sequences of low-light level images synchronized with recordings of membrane potential. The camera system is based on a cooled, scientific grade CCD camera controlled by a PC/AT computer. It can take 100 frames/sec of 18 X 18 element images and 4...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Lasser-Ross N,Miyakawa H,Lev-Ram V,Young SR,Ross WN

    更新日期:1991-02-01 00:00:00

  • EPI distortion correction for concurrent human brain stimulation and imaging at 3T.

    abstract:BACKGROUND:Transcranial magnetic stimulation (TMS) can be paired with functional magnetic resonance imaging (fMRI) in concurrent TMS-fMRI experiments. These multimodal experiments enable causal probing of network architecture in the human brain which can complement alternative network mapping approaches. Critically, me...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Oh H,Kim JH,Yau JM

    更新日期:2019-11-01 00:00:00

  • A computer program for sequencing and presenting complex sounds for auditory neuroimaging studies.

    abstract::This paper describes a computer program, named ASAP (audio sequencing and presentation), that runs on IBM PC compatible hardware with a Microsoft Windows operating system and has been specially designed for facilitating auditory neuroimaging studies. The program's main characteristics are: (1) the ability to generate ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kieckhefer E,Kanwal JS

    更新日期:2000-08-15 00:00:00

  • Detection of correlated sources in EEG using combination of beamforming and surface Laplacian methods.

    abstract::Beamforming offers a way to estimate the solution to the inverse problem in EEG and MEG but is also known to perform poorly in the presence of highly correlated sources, e.g. during binaural auditory stimulation, when both left and right primary auditory cortices are activated simultaneously. Surface Laplacian, or the...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Murzin V,Fuchs A,Scott Kelso JA

    更新日期:2013-08-15 00:00:00

  • Animal models for studying transport across the blood-brain barrier.

    abstract::There are many reasons for wishing to determine the rate of uptake of a drug from blood into brain parenchyma. However, when faced with doing so for the first time, choosing a method can be a formidable task. There are at least 7 methods from which to choose: indicator dilution, brain uptake index, microdialysis, exte...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Bonate PL

    更新日期:1995-01-01 00:00:00

  • In vivo cell type-specific CRISPR gene editing for sleep research.

    abstract::Sleep is an innate behavior conserved in all animals and, in vertebrates, is regulated by neuronal circuits in the brain. The conventional techniques of forward and reverse genetics have enabled researchers to investigate the molecular mechanisms that regulate sleep and arousal. However, functional interrogation of ge...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Yamaguchi H,de Lecea L

    更新日期:2019-03-15 00:00:00

  • Multi-lipofection efficiently transfected genes into astrocytes in primary culture.

    abstract::This study demonstrated that liposome-mediated transfection - lipofection - is suitable for delivering genes into astrocytes. By repeatedly lipofecting the same astrocyte cultures, a process we call multi-lipofection, the transfection efficiency of the beta-galactosidase (beta-gal) gene was improved from 2.6+/-0.6 to ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wu BY,Liu RY,So KL,Yu AC

    更新日期:2000-10-30 00:00:00

  • Fluorimetric determination of electrically evoked increase in intracellular calcium in cultured cerebellar granule cells.

    abstract::A technique is described to measure the electrically evoked increase in intracellular calcium in cerebellar granule cells cultured on glass coverslips and preloaded with FURA-2. To minimize light scattering, the coverslip containing the granules was placed in the fluorimeter cuvette at a 30 degrees angle to the exciti...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Beani L,Tomasini C,Govoni BM,Bianchi C

    更新日期:1994-01-01 00:00:00