Abstract：
:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological events with a temporal and spatial resolution not possible with AFM, while confocal imaging allows the simultaneous acquisition of immunofluorescence images. In this communication we present a simple method to combine AFM and confocal images to study differentiating embryonic stem (ES) cells-derived and dorsal root ganglia (DRG) neurons in culture. Neurons were grown on coverslips with micrometric markers that allow finding and imaging the same neuron with different microscopes. AFM and confocal images were registered using conventional methods used in Computer Science. The combination of these two techniques allows relating functional properties to morphological features of imaged neurons.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kondra S,Laishram J,Ban J,Migliorini E,Di Foggia V,Lazzarino M,Torre V,Ruaro MEdoi
10.1016/j.jneumeth.2008.09.034subject
Has Abstractpub_date
2009-02-15 00:00:00pages
94-107issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(08)00576-1journal_volume
177pub_type
杂志文章abstract：:A method for studying the process of regeneration in the frog sciatic nerve is presented. The outgrowth of sensory axons was determined by assaying the distribution of axonally transported radioactive proteins by fluorography of whole-mount nerve preparations. After labelling, the nerves were fixed at their in vivo le...
journal_title：Journal of neuroscience methods
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更新日期：1986-03-01 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1984-01-01 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2004-08-30 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2000-06-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.07.018
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00099-2
更新日期：2003-07-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.12.016
更新日期：2015-03-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.06.036
更新日期：2008-09-15 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：1997-06-06 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2010-03-30 00:00:00
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更新日期：2020-12-11 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：2011-10-15 00:00:00
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abstract：:Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...
journal_title：Journal of neuroscience methods
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