Integration of confocal and atomic force microscopy images.

Abstract:

:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological events with a temporal and spatial resolution not possible with AFM, while confocal imaging allows the simultaneous acquisition of immunofluorescence images. In this communication we present a simple method to combine AFM and confocal images to study differentiating embryonic stem (ES) cells-derived and dorsal root ganglia (DRG) neurons in culture. Neurons were grown on coverslips with micrometric markers that allow finding and imaging the same neuron with different microscopes. AFM and confocal images were registered using conventional methods used in Computer Science. The combination of these two techniques allows relating functional properties to morphological features of imaged neurons.

journal_name

J Neurosci Methods

authors

Kondra S,Laishram J,Ban J,Migliorini E,Di Foggia V,Lazzarino M,Torre V,Ruaro ME

doi

10.1016/j.jneumeth.2008.09.034

subject

Has Abstract

pub_date

2009-02-15 00:00:00

pages

94-107

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(08)00576-1

journal_volume

177

pub_type

杂志文章
  • Double labeling of cytochrome oxidase and gamma-aminobutyric acid in central nervous system neurons of adult cats.

    abstract::The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(89)90129-5

    authors: Luo XG,Hevner RF,Wong-Riley MT

    更新日期:1989-12-01 00:00:00

  • Modified herpes simplex virus delivery of enhanced GFP into the central nervous system.

    abstract::Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(02)00262-5

    authors: Sandler VM,Wang S,Angelo K,Lo HG,Breakefield XO,Clapham DE

    更新日期:2002-12-15 00:00:00

  • Physiological monitoring of small animals during magnetic resonance imaging.

    abstract::Maintaining a stable physiologic state is essential when studying animal models of epilepsy with simultaneous electroencephalograph (EEG) and functional magnetic resonance imaging (fMRI) or EEG and magnetic resonance spectroscopy (MRS). To achieve and maintain such stability in rats in the MRI environment, a minimally...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2004.11.019

    authors: Mirsattari SM,Bihari F,Leung LS,Menon RS,Wang Z,Ives JR,Bartha R

    更新日期:2005-06-15 00:00:00

  • Manipulation of PrPres production in scrapie-infected neuroblastoma cells.

    abstract::In the present study the accumulation of protease resistant prion protein (PrPres) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest levels of PrPres were found in slow growing cells. Further increases in PrPres accumulation were observed in ScN2a cells ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2004.04.001

    authors: Bate C,Langeveld J,Williams A

    更新日期:2004-09-30 00:00:00

  • Complexity in differentiating the expression of truncated or matured forms of MMP-2 and MMP-9 through zymography in rat brain tissues after acute ischaemic stroke.

    abstract::Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of ischaemic stroke. In particular, the mature forms of MMPs 2 and 9 have similar sizes and share gelatine as a common substrate. Both MMPs are upregulated in ischaemic stroke and play detrimental roles during stroke pathogenesis. Throughout t...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.03.007

    authors: Alam M,Shuaib A

    更新日期:2013-05-30 00:00:00

  • Methodological approaches to exploring epileptic disorders in the human brain in vitro.

    abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/j.jneumeth.2006.04.009

    authors: Köhling R,Avoli M

    更新日期:2006-07-15 00:00:00

  • Fluorimetric determination of electrically evoked increase in intracellular calcium in cultured cerebellar granule cells.

    abstract::A technique is described to measure the electrically evoked increase in intracellular calcium in cerebellar granule cells cultured on glass coverslips and preloaded with FURA-2. To minimize light scattering, the coverslip containing the granules was placed in the fluorimeter cuvette at a 30 degrees angle to the exciti...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(94)90020-5

    authors: Beani L,Tomasini C,Govoni BM,Bianchi C

    更新日期:1994-01-01 00:00:00

  • Susceptibility artifact correction for sub-millimeter fMRI using inverse phase encoding registration and T1 weighted regularization.

    abstract:BACKGROUND:Functional magnetic resonance imaging (fMRI) enables non-invasive examination of both the structure and the function of the human brain. The prevalence of high spatial-resolution (sub-millimeter) fMRI has triggered new research on the intra-cortex, such as cortical columns and cortical layers. At present, ec...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108625

    authors: Duong STM,Phung SL,Bouzerdoum A,Boyd Taylor HG,Puckett AM,Schira MM

    更新日期:2020-04-15 00:00:00

  • Numerical simulation of nonlinear feedback model of saccade generation circuit implemented in the LabView graphical programming language.

    abstract::The object-oriented graphical programming language LabView was used to implement the numerical solution to a computational model of saccade generation in primates. The computational model simulates the activity and connectivity of anatomical strictures known to be involved in saccadic eye movements. The LabView progra...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(98)00171-x

    authors: Jackson ME,Gnadt JW

    更新日期:1999-03-01 00:00:00

  • Neuromuscular electrical stimulation of the hindlimb muscles for movement therapy in a rodent model.

    abstract::Neuromuscular electrical stimulation (NMES) can provide functional movements in people after central nervous system injury. The neuroplastic effects of long-term NMES-induced repetitive limb movement are not well understood. A rodent model of neurotrauma in which NMES can be implemented may be effective for such inves...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2008.09.015

    authors: Ichihara K,Venkatasubramanian G,Abbas JJ,Jung R

    更新日期:2009-01-30 00:00:00

  • Computer-driven automatic identification of locomotion states in Caenorhabditis elegans.

    abstract::We developed a computer-driven tracking system for the automated analysis of the locomotion of Caenorhabditis elegans. The algorithm for the identification of locomotion states on agar plates (forward movement, backward movement, rest, and curl) includes the identification of the worm's head and tail. The head and tai...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2006.05.002

    authors: Hoshi K,Shingai R

    更新日期:2006-10-30 00:00:00

  • A microassay for simultaneous measurement of in vivo rates of tryptophan hydroxylation and levels of serotonin in discrete brain nuclei.

    abstract::A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibitio...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(82)90015-2

    authors: Long JB,Youngblood WW,Kizer JS

    更新日期:1982-07-01 00:00:00

  • A low-cost, portable, micro-controlled device for multi-channel LED visual stimulation.

    abstract::Light emitting diodes (LEDs) are extensively used as light sources to investigate visual and visually related function and dysfunction. Here, we describe the design of a compact, low-cost, stand-alone LED-based system that enables the configuration, storage and presentation of elaborate visual stimulation paradigms. T...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2011.02.004

    authors: Pinto MA,de Souza JK,Baron J,Tierra-Criollo CJ

    更新日期:2011-04-15 00:00:00

  • An inexpensive and interactive microcomputer system for codifying Golgi-impregnated neuronal morphology.

    abstract::An interactive microcomputer system has been developed for the quantitative analysis of Golgi-impregnated neuronal morphology (GINM). The system uses commercially available hardware: a 48K RAM microcomputer (Apple II), a TV monitor (NEC), an optical microscope (Reichert Biovar) with a camera lucida and a specially dev...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(86)90043-9

    authors: Freire M

    更新日期:1986-04-01 00:00:00

  • On the use of ibotenic acid to lesion selectively different components of the hippocampal formation.

    abstract::Procedures are described for lesioning two components of the hippocampal formation (hippocampus, subiculum) using multiple injections of small amounts of ibotenic acid (IBO). The resulting loss of cells is more selective and limited than can be obtained with conventional techniques. Thus, problems associated with dama...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(89)90149-0

    authors: Jarrard LE

    更新日期:1989-09-01 00:00:00

  • Factors affecting the stability of the spike-triggered averaged force in the human first dorsal interosseus muscle.

    abstract::The reproducibility of motor unit twitches obtained using spike-triggered averaging (STA) was examined in the human first dorsal interosseus. For each motor unit (30 total) a series of STA twitches was derived using a 30 s averaging window. Within each averaging window, eight independent measures characterizing motor ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(03)00077-3

    authors: Gossen ER,Ivanova TD,Garland SJ

    更新日期:2003-06-30 00:00:00

  • Caloric stimulation of ampullar receptors: a new method to produce mechanically-evoked responses in frog semicircular canals.

    abstract::A microthermistor positioned close to the exposed posterior semicircular canal in isolated labyrinth preparations of the frog was used to stimulate the sensory organ. Our results indicated that, depending on the position of the heater, the induced endolymphatic convection currents may result in either excitatory or in...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/s0165-0270(99)00021-7

    authors: Zucca G,Botta L,Valli S,Giannoni B,Mira E,Perin P,Valli P

    更新日期:1999-05-01 00:00:00

  • Quantification of neurons in the myenteric plexus: an evaluation of putative pan-neuronal markers.

    abstract::Accurate estimates of the total number of neurons located in the wall of the gut are essential for studies of the enteric nervous system (ENS). Though several stains and antibodies are used routinely as pan-neuronal markers, controversies of relative sensitivity and completeness have been difficult to resolve, at leas...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2003.10.004

    authors: Phillips RJ,Hargrave SL,Rhodes BS,Zopf DA,Powley TL

    更新日期:2004-02-15 00:00:00

  • A novel microsurgical nerve implantation technique preserving outer nerve layers.

    abstract::A novel epineural tube implantation paradigm in the adult rat was designed for the analysis of regulatory cell interactions in peripheral nerves and for the development of therapeutic implants. The aim was to allow the integration of synthetic regenerative structures and cells into the nerve interior while preserving ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2010.04.007

    authors: Hajosch R,Grupp L,Nichterwitz S,Schlosshauer B

    更新日期:2010-06-15 00:00:00

  • An integrative transcranial magnetic stimulation mapping technique using non-linear curve fitting.

    abstract::The aim of this study is to develop a new simple method for analyzing one-dimensional transcranial magnetic stimulation (TMS) mapping studies in humans. Motor evoked potentials (MEP) were recorded from the abductor pollicis brevis (APB) muscle during stimulation at nine different positions on the scalp along a line pa...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2006.04.018

    authors: Kohl AS,Conforto AB,Z'Graggen WJ,Kaelin-Lang A

    更新日期:2006-10-30 00:00:00

  • An automated method for autoradiographic analysis of cultured Schwann cells.

    abstract::A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(87)90062-8

    authors: Baichwal RR,Yan L,Bosler A,DeVries GH

    更新日期:1987-08-01 00:00:00

  • Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice.

    abstract:BACKGROUND:PET allows the measurement of CBF, CBV and CMRO2 in human and plays an important role in the diagnosis of pathologic conditions and clinical research. On the other hand, in animal studies, there is no optical imaging system for evaluating changes in CBF and CBV, and oxygen metabolism, from the same brain are...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2014.08.022

    authors: Takuwa H,Matsuura T,Nishino A,Sakata K,Tajima Y,Ito H

    更新日期:2014-11-30 00:00:00

  • Antagonism of haloperidol-induced swim impairment in L-dopa and caffeine treated mice: a pre-clinical model to study Parkinson's disease.

    abstract::Parkinson's disease (PD) exhibits symptoms of motor dysfunction such as tremor, akinesia and rigidity. Agents that selectively disrupt or destroy catecholaminergic systems, such as reserpine, methamphetamine, 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, have been used to develop PD models and to...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2008.12.019

    authors: Luthra PM,Barodia SK,Raghubir R

    更新日期:2009-04-15 00:00:00

  • The sympathoadrenal system, obesity and hypertension: an overview.

    abstract::Obesity-related hypertension is a clinical problem of major significance. The nature of the relationship between blood pressure and body weight has not been elucidated. Recent studies suggest that insulin (and/or insulin resistance) may be involved. An hypothesis is developed, based on the relationship between dietary...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审

    doi:10.1016/0165-0270(90)90056-l

    authors: Landsberg L

    更新日期:1990-09-01 00:00:00

  • The feasibility of quantitative MRI of perivascular spaces at 7T.

    abstract:BACKGROUND:Dilated brain perivascular spaces (PVSs) are found to be associated with many conditions, including aging, dementia, and Alzheimer's disease (AD). Conventionally, PVS assessment is mainly based on subjective observations of the number, size and shape of PVSs in MR images collected at clinical field strengths...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2015.09.001

    authors: Cai K,Tain R,Das S,Damen FC,Sui Y,Valyi-Nagy T,Elliott MA,Zhou XJ

    更新日期:2015-12-30 00:00:00

  • Modeling the dopaminergic nerve terminal.

    abstract::A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(88)90045-3

    authors: Justice JB Jr,Nicolaysen LC,Michael AC

    更新日期:1988-01-01 00:00:00

  • A rapid fluorescent method to quantify neuronal loss after experimental intracerebral hemorrhage.

    abstract::Neuronal loss in tissue surrounding an intracerebral hemorrhage (ICH) is usually quantified by labor-intensive histological methods that are subject to bias. Fluorescent protein expression has been successfully used as a marker of cell viability in vitro and in retinal studies in vivo, but not in any ICH model to date...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2013.03.025

    authors: Chen-Roetling J,Lu X,Regan KA,Regan RF

    更新日期:2013-06-15 00:00:00

  • Synaptic release of zinc from brain slices: factors governing release, imaging, and accurate calculation of concentration.

    abstract::Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of pub...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2005.11.014

    authors: Frederickson CJ,Giblin LJ 3rd,Balaji RV,Masalha R,Frederickson CJ,Zeng Y,Lopez EV,Koh JY,Chorin U,Besser L,Hershfinkel M,Li Y,Thompson RB,Krezel A

    更新日期:2006-06-30 00:00:00

  • A kernel-based method to calculate local field potentials from networks of spiking neurons.

    abstract:BACKGROUND:The local field potential (LFP) is usually calculated from current sources arising from transmembrane currents, in particular in asymmetric cellular morphologies such as pyramidal neurons. NEW METHOD:Here, we adopt a different point of view and relate the spiking of neurons to the LFP through efferent synap...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/j.jneumeth.2020.108871

    authors: Telenczuk B,Telenczuk M,Destexhe A

    更新日期:2020-10-01 00:00:00

  • Gaze control in cats achieved by rewarding tongue push movements during the flickering of a light-emitting diode.

    abstract::A method is described for achieving gaze control in cats, by rewarding them for pushing against a lever with their tongue, only when a light-emitting diode (LED) was flickering. Since the state of the LED was changed sequentially from OFF to ON to flickering and the duration of flickering was short, the cats were requ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章

    doi:10.1016/0165-0270(80)90036-9

    authors: Hamada T

    更新日期:1980-10-01 00:00:00