l-Glutamate in Middlebrook 7H9 culture medium upregulates matrix metalloproteinase-2 secretion from human astrocytoma cells.

Abstract:

:Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not stimulate MMP-1 or MMP-9 secretion from astrocytoma cells. The excitatory neurotransmitter l-glutamate, at concentrations found in Middlebrook 7H9 media, induced significant astrocytoma MMP-2 secretion (p<0.05). l-Glutamate-induced MMP-2 activity may contribute to neuropathology in various CNS diseases and may generate misleading data in pathogen studies where Middlebrook 7H9 is the culture medium.

journal_name

J Neurosci Methods

authors

Harris JE,Friedland JS

doi

10.1016/j.jneumeth.2008.06.007

subject

Has Abstract

pub_date

2008-08-30 00:00:00

pages

291-4

issue

2

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(08)00341-5

journal_volume

173

pub_type

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