Abstract：
:The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at sufficiently high pressures (above about 100 kPa) and for pipettes with a sufficiently high hydrodynamic conductance (larger than 1 pl s-1 kPa-1 at 230 kPa). In this range, flows were found with low Reynolds numbers (smaller than 10), which is consistent with laminar flows. For all but the largest micropipettes, the relationship between pressure and ejected volume is alinear: the pipettes' apparent hydrodynamic conductances increase with increasing pressure. Micropipettes with apparent hydrodynamic conductances between 0.04 and 1400 pl s-1 kPa-1 (at 230 kPa) were tested. Duration-pressure combinations could be defined where the duration-volume relationship was either linear or monotonic. such duration-pressure combinations were different for pipettes with different apparent hydrodynamic conductances. A quick method is described to measure the pipette's apparent hydrodynamic conductance at the pressure used, corrected for the fluid's viscosity. Measurement of this conductance permits predictable injections of known volumes of fluid in the range of 100 pl to 1 microliter with a precision of 10-20%.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
van Dongen PAdoi
10.1016/0165-0270(84)90021-9subject
Has Abstractpub_date
1984-04-01 00:00:00pages
281-91issue
4eissn
0165-0270issn
1872-678Xpii
0165-0270(84)90021-9journal_volume
10pub_type
杂志文章abstract：BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.015
更新日期：2015-05-15 00:00:00
abstract：:Binding of the cocaine analog 3 beta-(4-[125I]iodophenyl)tropane-2 beta-carboxylic acid isopropyl ester ([125I]RTI-121) to filters was studied in order to assess its contribution to labeling dopamine transporters on rat striatal synaptosomal membranes in filtration assays. Filter binding (FB) decreased with increasing...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00070-8
更新日期：1997-08-22 00:00:00
abstract：:Parkinson's disease (PD) has a long prodromal period that precedes the appearance of typical motor symptoms. This phase is extremely heterogeneous and is characterized by a wide range of non-motor alterations including REM sleep behavior disorders, constipation, olfactory and urinary dysfunctions. The increasing clini...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2020.108801
更新日期：2020-08-01 00:00:00
abstract：:The technique named detrended fluctuation analysis (DFA) has been used to reveal the presence of long-range temporal correlations (LRTC) and scaling behavior (SB) in electroencephalographic (EEG) recordings. The occurrence of these phenomena seems to be a salient characteristic of the healthy human brain and alteratio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.010
更新日期：2008-05-30 00:00:00
abstract：:Presented in this short communication are design principles for the construction of a positioning apparatus for biological specimens in magnetically sensitive environments. An apparatus with 3 degrees of freedom was built and found to provide position accuracy to within +/- 0.25 mm throughout a cubic volume measuring ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90112-q
更新日期：1992-08-01 00:00:00
abstract：:We developed a high speed voice coil based whisker stimulator that delivers precise deflections of a single whisker or group of whiskers in a repeatable manner. The device is miniature, quiet, and inexpensive to build. Multiple stimulators fit together for independent stimulation of four or more whiskers. The system c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.008
更新日期：2011-03-15 00:00:00
abstract：:The scanning electron microscope (SEM) is a useful tool in the study of the retina of lower vertebrates. SEM analysis (preparation over the critical point) offers a useful addition to the existing histological preparations. It is suitable for a comparative analysis, in which the arrangement of different cellular eleme...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90049-9
更新日期：1984-11-01 00:00:00
abstract：BACKGROUND:Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the ana...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.02.011
更新日期：2016-05-01 00:00:00
abstract：:The implantation of a foreign object in the brain produces an acute neuroinflammatory state in which glia (astrocytes and microglia) may remain chronically activated in response to the inert foreign object. Activated glia can exhibit a sensitized pro-inflammatory response to immunogenic stimuli. This may be relevant t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.11.014
更新日期：2007-04-15 00:00:00
abstract：:Trace amounts of [14C]2-deoxyglucose (2-DG) were used to detect regions of the brainstem involved in forelimb stepping in thalamic and low spinal cats. Under ether anesthesia, cats were transected at the stereotaxic A12 level and T10 segment. Two hours later, 50 microCi/kg of 2-DG was infused i.v. and one of 4 procedu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90124-5
更新日期：1987-10-01 00:00:00
abstract：:This paper overviews the design and implementation of three neuromorphic integrated circuits developed for the COLAMN ("Novel Computing Architecture for Cognitive Systems based on the Laminar Microcircuitry of the Neocortex") project. The circuits are implemented in a standard 0.35 μm CMOS technology and include spiki...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.01.019
更新日期：2012-09-15 00:00:00
abstract：:Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the rapid degradation of fatty acid amides such as the endocannabinoid anandamide. Inhibition of FAAH activity has been suggested as a therapeutic approach for the treatment of chronic pain, depression and anxiety, through local activation of the cannabin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.10.006
更新日期：2007-03-30 00:00:00
abstract：:Hippocampal slices were obtained from hippocampi of patients with temporal lobe epilepsy or from patients with mass lesions located in the temporal lobe. Hippocampal slices were kept alive in a slice chamber and the anterograde tracer neurobiotin was iontophoretically injected into the granule cell layer of the dentat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00187-5
更新日期：2002-10-15 00:00:00
abstract：:Light emitting diodes (LEDs) are extensively used as light sources to investigate visual and visually related function and dysfunction. Here, we describe the design of a compact, low-cost, stand-alone LED-based system that enables the configuration, storage and presentation of elaborate visual stimulation paradigms. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.02.004
更新日期：2011-04-15 00:00:00
abstract：:In many experimental designs, animal observation is associated with local field potential (LFP) recordings in order to find correlations between behavior dynamics and neuronal activity. In such cases, relevant behaviors can occur at different times during free-running recordings and should be put together by the time ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.08.027
更新日期：2008-01-30 00:00:00
abstract：BACKGROUND:Primary astrocyte cultures have been used for decades to study astrocyte functions in health and disease. The current primary astrocyte cultures are mostly maintained in serum-containing medium which produces astrocytes with a reactive phenotype as compared to in vivo quiescent astrocytes. The aim of this st...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.03.013
更新日期：2019-05-15 00:00:00
abstract：BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.10.009
更新日期：2014-01-15 00:00:00
abstract：:The aim of the study was to compare saccadic peak velocity (SPV) values measured with video based Fitness Impairment Tester (FIT) and electro-oculography (EOG) during prolonged wakefulness. We tested different numbers of saccades and two saccade paradigms to improve the EOG measurements for detecting fatigue. The SPVs...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.01.010
更新日期：2010-03-30 00:00:00
abstract：:The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for unbiased estimation of anisotropic surfaces. The examples include descriptions of estimators of neurone and synapse number and siz...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90112-3
更新日期：1986-10-01 00:00:00
abstract：BACKGROUND:Spike-wave discharges (SWD) found in neuroelectrical recordings are pathognomonic to absence epilepsy. The characteristic spike-wave morphology of the spike-wave complex (SWC) constituents of SWDs can be mathematically described by a subset of possible spectral power and phase values. Morlet wavelet transfor...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.12.016
更新日期：2015-03-15 00:00:00
abstract：:The brain is a complex neural network with interleaving functional connectivity among anatomical regions. However, current functional parcellation algorithms usually emphasize independence or orthogonality between the spatial components, with the interleaving nature underrepresented. This study investigates a method, ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.05.001
更新日期：2011-07-15 00:00:00
abstract：:Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00352-6
更新日期：2001-04-30 00:00:00
abstract：:A method is described for the production of an electrode capable of monitoring and modulating the input-output relationship of thalamic neurons. Tungsten-in-glass electrodes were manufactured with the ability to simultaneously record lateral geniculate nucleus action potentials and associated, retinally evoked S-poten...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90126-c
更新日期：1993-09-01 00:00:00
abstract：BACKGROUND:Delivering solutes to a particular region of the brain is currently achieved by iontophoresis for very small volumes and by diffusion from a microdialysis probe for larger volumes. There is a need to deliver solutes to particular areas with more control than is possible with existing methods. NEW METHOD:Ele...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.005
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND:Transcranial magnetic stimulation (TMS) can be paired with functional magnetic resonance imaging (fMRI) in concurrent TMS-fMRI experiments. These multimodal experiments enable causal probing of network architecture in the human brain which can complement alternative network mapping approaches. Critically, me...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108400
更新日期：2019-11-01 00:00:00
abstract：:We describe a modification of the Harper-McGinty microdrive for use with extracellular, microwire recording in conscious, chronically prepared rabbits. Improvements over existing designs are in ease of assembly and mechanical stability. The microdrive is easily fabricated with hand tools and readily available parts. I...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.10.020
更新日期：2006-06-15 00:00:00
abstract：:We have developed a labeling procedure which accurately and consistently labels the original sensory pools projecting to their respective nerve branches as a model to quantify the accuracy of nerve regeneration at the single neuron level. Adult and juvenile rats had the saphenous branch of the femoral nerve transected...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90078-e
更新日期：1991-09-01 00:00:00
abstract：:Most spinal cord injury research is undertaken using in vivo animal models but the extensive care associated with spinalized animals, inherent variability between animals, and complex surgeries makes alternative models especially valuable. Here we present a novel ex vivo model that enables culture of intact post-natal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.07.012
更新日期：2010-09-30 00:00:00
abstract：:Triple immunofluorescence method with two mouse monoclonal antibodies and another rabbit polyclonal antibody was established with catalyzed reporter deposition (CARD) amplification on thick floating sections from the rat cerebellum. One of the monoclonal antibodies (anti-calbindin), diluted maximally, probed with anti...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.12.003
更新日期：2004-05-30 00:00:00
abstract：:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.010
更新日期：2009-03-30 00:00:00
