Abstract:
:A Lucite plate reconstruction method is described which, when combined with HRP histochemistry, provides an excellent means of visualizing, in 3-dimensional fashion, the functional organization of neuronal populations. Color photomicrographs of representative serial sections were made through the baboon oculomotor nucleus. Each color slide was then projected onto a 9 X 12 in. Lucite plate and the configuration of the nucleus at each representative level drawn to scale on the plates. These plates were then stacked one in front of the other yielding a see-through, 3-dimensional reconstruction of the entire nucleus. Color transparencies of every sixth HRP-processed section were made and the image of each section projected onto the Lucite plates on which the configuration of the oculomotor nucleus was previously outline. Using different colored stars to represent the neurons which supply different oculomotor muscles, the number and location of HRP-positive neurons was then plotted. The end result was an anatomically accurate 3-dimensional reconstruction of the entire labeled population of the oculomotor nucleus including the location of subnuclei that can be viewed from any side or at any angle. With adequate reference points these data can be entered into a computer graphics device, then viewed and manipulated in 3-dimensional fashion.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Augustine JRdoi
10.1016/0165-0270(81)90019-4subject
Has Abstractpub_date
1981-06-01 00:00:00pages
63-71issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(81)90019-4journal_volume
4pub_type
杂志文章abstract::This paper describes a simple multichannel hook electrode specially adapted for simultaneous recording from several dorsal root filaments in the cat. The electrode consists of a 3-mm-thick PVC plate and accommodates 12 silver wire hooks placed in notches in the plate. It has a semicircular shape, and the notches are m...
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1984-02-01 00:00:00