Abstract:
:Microdialysis is a useful tool for administering drugs into localized regions of brain tissue, but the diffusion of drugs from the probe has not been systematically examined. Lidocaine (10%) and tetrodotoxin (TTX, 10 microM), drugs typically used in neural inactivation studies, were infused through a microdialysis probe into raccoon somatosensory cortex while evoked responses were recorded at four electrodes equally spaced 0.5--2.0 mm from the probe. The decreases in evoked response amplitude as a function of time and distance from the probe were used as functional measures to describe the time course and spread of the drugs. TTX inactivated distant sites more quickly and to a greater extent than lidocaine. Responses recovered within approximately 40 min after termination of lidocaine, but did not recover for at least 2 h after TTX. Based on these measurements, we estimated that, at the concentrations used, lidocaine has a maximal spread of 2.1 mm, while TTX could spread as far as 4.8 mm from the microdialysis probe. However, in terms of significant inactivation of neuronal activity, lidocaine and TTX have an effective spread of 1 and 2 mm, respectively.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Boehnke SE,Rasmusson DDdoi
10.1016/s0165-0270(00)00348-4subject
Has Abstractpub_date
2001-02-15 00:00:00pages
133-41issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(00)00348-4journal_volume
105pub_type
杂志文章abstract:BACKGROUND:Multiscale entropy (MSE) estimates the predictability of a signal over multiple temporal scales. It has been recently applied to study brain signal variability, notably during aging. The grounds of its application and interpretation remain unclear and subject to debate. METHOD:We used both simulated and exp...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.09.004
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:This article addresses the automatic classification of reconstructed neurons through their morphological features. The purpose was to extend the capabilities of the L-Measure software. METHODS:New morphological features were developed, based on modifications of the conventional Sholl analysis. The lengths o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108835
更新日期:2020-09-01 00:00:00
abstract::We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We corr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90007-3
更新日期:1990-01-01 00:00:00
abstract::A method which improves carbon fiber microelectrode selectivity for cationic amines by electrocoating the fiber with a thin film of the ionic polymer, Nafion, is described. The selectivity and response speed of these electrodes for the detection of electroactive cationic and anionic species found in brain extracellula...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90011-2
更新日期:1987-12-01 00:00:00
abstract::Paper-pencil type tests are traditionally used in the assessment of executive functions (EF); however, concerns have been raised as to whether these represent actual functioning in everyday life. Virtual reality (VR) environments offer a novel alternative for the assessment of cognitive function and therefore have the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.07.005
更新日期:2013-09-30 00:00:00
abstract::A technique has been developed for recording single unit afferent activity from mesenteric nerves in isolated segments of rat distal ileum in vitro. The preparation consists of a 3-cm segment of ileum, containing a single neurovascular bundle, held horizontally in an organ bath. One end of the segment is attached to a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90047-6
更新日期:1986-04-01 00:00:00
abstract::Biocytin is a biotin-lysine complex of low molecular weight containing about 65% biotin, which retains a high affinity for avidin. Since the latter molecule has been conjugated to several histochemical markers, the use of biocytin as an intracellular marker was investigated. Electrodes were filled with a solution of 4...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90114-8
更新日期:1988-08-01 00:00:00
abstract::In the three-dimensional reconstruction of neuronal structure, it is imperative that ribbons of semithin or ultrathin sections be obtained. Resin-embedded semithin sections display better structural details than paraffin-embedded sections. The cutting and collecting of long ribbons of resin-embedded semithin sections ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90179-3
更新日期:1988-03-01 00:00:00
abstract::A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been established for the rapid and reliable determination of gamma-aminobutyric acid (GABA) and glutamate in brain microdialysates. The microdialysis samples were analysed using a HILIC (hydrophilic interaction liquid chromatography) column, which ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.05.018
更新日期:2009-08-30 00:00:00
abstract:BACKGROUND:The myelin sheath produced by glial cells insulates the axons, and supports the function of the nervous system. Myelin sheath degeneration causes neurodegenerative disorders, such as multiple sclerosis (MS). There are no therapies for MS that promote remyelination. Drug discovery frequently involves screenin...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108946
更新日期:2020-12-01 00:00:00
abstract::In rats with unilateral 6-hydroxydopamine lesions of the substantia nigra apomorphine reliably induces a response of contralateral rotation. The present study shows that this response can readily be conditioned to a test environment which the rats are briefly exposed to during the onset of the apomorphine-induced rota...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90046-5
更新日期:1988-01-01 00:00:00
abstract::The effects of the microwave antigen-retrieval method were investigated for five antibodies commonly used in neurosciences, i.e., Parvalbumin, Calbindin D28-K, MAP-2, MAP-5 and SMI-32. Immunocytochemical stainings were performed on free-floating vibratome sections of tissue which had been stored in fixative for as lon...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)90208-9
更新日期:1994-12-01 00:00:00
abstract::There is an accumulating literature demonstrating the application of microwaves across a wide spectrum of histological techniques. Although exposure to microwaves for short periods resulted in substantial acceleration of all procedures this technique still is not adopted widely. In part, this may be due to concerns ov...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.07.002
更新日期:2013-09-30 00:00:00
abstract::Children and adolescents with attention-deficit/hyperactivity disorder (ADHD) have smaller cerebellar volumes, particularly in the posterior-inferior cerebellar vermis (lobules VIII-X). Functional activation of the human cerebellar vermis following stimulant administration has also been repeatedly demonstrated. There ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.019
更新日期:2006-02-15 00:00:00
abstract::A simple and low-cost device is described which facilitates the calibration of intracellular DC recordings in amplitude and time. A current source, optically coupled to a conventional stimulator, injects a pulse of current, across a 1-omega resistor connecting the preparation to ground. Amplitude and duration of the c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(84)90008-6
更新日期:1984-05-01 00:00:00
abstract::Display systems based on conventional computer graphics cards are capable of generating images with about 8-bit luminance resolution. However, most vision experiments require more than 12 bits of luminance resolution. Pelli and Zhang [Spatial Vis. 10 (1997) 443] described a video attenuator for generating high luminan...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(03)00174-2
更新日期:2003-11-30 00:00:00
abstract::Analysis of cortical patterns requires accurate cortical surface registration. Many researchers map the cortical surface onto a unit sphere and perform registration of two images defined on the unit sphere. Here we have developed a novel registration framework for the cortical surface based on spherical thin-plate spl...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.02.010
更新日期:2012-04-30 00:00:00
abstract::Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00057-0
更新日期:1998-08-01 00:00:00
abstract::The detection of epileptiform discharges in the EEG is an important component in the diagnosis of epilepsy. In this study, multiple signal classification (MUSIC), autoregressive (AR) and periodogram methods were used to get power spectra in patients with absence seizure. The EEG power spectra were used as an input to ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.04.009
更新日期:2005-10-30 00:00:00
abstract::A new method to study the reactions to unexpected mechanical perturbations during human walking on a treadmill is presented. Perturbations consisted of an obstruction of the forward swinging foot during the early swing phase. These were caused by obstacles which were dropped on the treadmill in front of the subject. T...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00149-2
更新日期:1996-07-01 00:00:00
abstract::The event-related potential (ERP) and event-related field (ERF) techniques provide valuable insights into the time course of processes in the brain. Because neural signals are typically weak, researchers commonly filter the data to increase the signal-to-noise ratio. However, filtering may distort the data, leading to...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.06.011
更新日期:2012-07-30 00:00:00
abstract::Obesity-related hypertension is a clinical problem of major significance. The nature of the relationship between blood pressure and body weight has not been elucidated. Recent studies suggest that insulin (and/or insulin resistance) may be involved. An hypothesis is developed, based on the relationship between dietary...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(90)90056-l
更新日期:1990-09-01 00:00:00
abstract::So far, the double-magnetic induction (DMI) method has been successfully applied to record eye movements from head-restrained humans, monkeys and cats. An advantage of the DMI method, compared to the more widely used scleral search coil technique, is the absence of vulnerable lead wires on the eye. A disadvantage, how...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.08.012
更新日期:2007-02-15 00:00:00
abstract::Enzymatically isolated retinal glial (Müller) cells have been the subject of many electrophysiological studies. Local high membrane conductivities for potassium ions have been speculated to correspond with local occurrence of orthogonal arrays of intramembranous particles (OAP) observed in freeze-fracture replicas of ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90145-6
更新日期:1990-06-01 00:00:00
abstract::The aim of the study was to compare saccadic peak velocity (SPV) values measured with video based Fitness Impairment Tester (FIT) and electro-oculography (EOG) during prolonged wakefulness. We tested different numbers of saccades and two saccade paradigms to improve the EOG measurements for detecting fatigue. The SPVs...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.01.010
更新日期:2010-03-30 00:00:00
abstract::The coupling of Ca(2+) influx to synaptic vesicle (SV) recycling in nerve terminals is essential for neurotransmitter release and thus neuronal communication. Both of these parameters have been monitored using fluorescent reporter dyes such as fura-2 and FM1-43 in single central nerve terminals. However, their simulta...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.09.012
更新日期:2007-03-15 00:00:00
abstract::The FD Rapid GolgiStain Kit (FD NeuroTechnologies, Inc., Ellicott City, MD) was developed using an improved Golgi-Cox impregnation method to provide stable, sensitive, and convenient staining of mature neurons. However, embryonic neurons cannot be clearly visualized using this method. We have further improved the Golg...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.06.007
更新日期:2012-07-30 00:00:00
abstract:BACKGROUND:Epileptic seizures evolve through several states, and in the process the brain signals may change dramatically. Signals from different states share similar features, making it difficult to distinguish them from a time series; the goal of this work is to build a classifier capable of identifying seizure state...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.12.001
更新日期:2015-02-15 00:00:00
abstract::Recent studies highlighted the disagreement between the typical number of neurons observed with extracellular recordings and the ones to be expected based on anatomical and physiological considerations. This disagreement has been mainly attributed to the presence of sparsely firing neurons. However, it is also possibl...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.07.010
更新日期:2012-10-15 00:00:00
abstract::The present work introduces an electrode microdrive system small enough to be placed into two distinct brain areas in head-fixed mice. To meet the space constraint imposed by the size of mice and the additional presence of a head post, the size and weight of the components were minimized. In the current version, an in...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.12.015
更新日期:2010-03-15 00:00:00