Abstract：
:The present work introduces an electrode microdrive system small enough to be placed into two distinct brain areas in head-fixed mice. To meet the space constraint imposed by the size of mice and the additional presence of a head post, the size and weight of the components were minimized. In the current version, an individual microdrive moves an array of four Reitboeck type electrodes. We report about successful implantation in rats and mice using one or two microdrives. Using two of these devices in individual mice/rats, the recording of parallel single and multi-unit as well as local field potential from prefrontal, motor, somatosensory cortex and hippocampus is demonstrated. The system can be easily constructed with machinery and equipment present in most neurophysiology labs.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Haiss F,Butovas S,Schwarz Cdoi
10.1016/j.jneumeth.2009.12.015subject
Has Abstractpub_date
2010-03-15 00:00:00pages
67-72issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(09)00680-3journal_volume
187pub_type
杂志文章abstract：:In this article, we describe an efficient method for testing both auditory receptor and auditory nerve function in a single recording session. Auditory receptor function is tested in response to pure tone, tone burst and click acoustic stimuli (i.e. distortion products of otoacoustic emissions, DPOAE; and auditory-evo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.11.003
更新日期：2004-04-30 00:00:00
abstract：:Images of Golgi impregnated neurons from different laminae of the human and rat dorsal horns were subjected to a quantitative analysis to support the Rexed's laminar scheme in mammals. Four methods of fractal analysis were performed in the proceedings: box-counting, mass-radius, cumulative intersection, and vectorized...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.02.009
更新日期：2005-08-15 00:00:00
abstract：:Sensory and motor pathways in the central nervous system (CNS) of macaque monkeys were visualized by anterograde or retrograde axonal transport of wheatgerm agglutinin-horseradish peroxidase (WGA-HRP) reacted with the chromagen tetramethylbenzidine (TMB), or by the use of anterograde degeneration after specific ablati...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00117-y
更新日期：1995-02-01 00:00:00
abstract：:Microdialysis is a useful tool for administering drugs into localized regions of brain tissue, but the diffusion of drugs from the probe has not been systematically examined. Lidocaine (10%) and tetrodotoxin (TTX, 10 microM), drugs typically used in neural inactivation studies, were infused through a microdialysis pro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00348-4
更新日期：2001-02-15 00:00:00
abstract：BACKGROUND:Deviant stimuli within a standard, frequent stimulus train induce a cortical somatosensory mismatch response (SMMR). The SMMR reflects the brain's automatic mechanism for the detection of change in a somatosensory domain. It is usually elicited by electrical stimulation, which activates nerve fibers and rece...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.09.014
更新日期：2019-01-01 00:00:00
abstract：:A video-based 2D eye-tracking system (EyeLink version 2.04, SR Research Ltd/SMI) was compared with the scleral search coil technique for its performance on recording the properties of fixations and saccadic eye movements. Fixation positions and saccadic properties (amplitude, duration, and peak velocity) were calculat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00527-1
更新日期：2002-03-15 00:00:00
abstract：:Chronic spinal catheterization via an atlanto-occipital puncture (CAO) has been widely used to study the effects of drugs on spinal nociceptive mechanisms, but this method is associated with spinal cord damage that may change the efficacy of spinally injected analgesics. Using a slight modification of the method of St...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00197-3
更新日期：2003-10-15 00:00:00
abstract：BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108903
更新日期：2020-11-01 00:00:00
abstract：:Quantifying the connectivity between arbitrary surface patches in the human brain cortex can be used in studies on brain function and to characterize clinical diseases involving abnormal connectivity. Cortical regions of human brain in their natural forms can be represented in surface formats. In this paper, we presen...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneumeth.2011.02.017
更新日期：2011-04-30 00:00:00
abstract：:We developed a system for quantitatively measuring arm movement. Our approach provides a method to simultaneously capture upper limb kinetic and kinematic data during assisted passive arm movements. Data are analysed with respect to Cartesian and upper limb coordinate systems to obtain upper limb joint angles and torq...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.03.004
更新日期：2011-04-30 00:00:00
abstract：:Previously, we developed technology that coupled high-speed chronoamperometry with microejections of dopamine (DA) to measure DA clearance in the brains of freely-behaving rats. Here, by varying the ejection volumes of DA across a 200-fold difference, the kinetics of striatal clearance were analyzed as a function of t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00229-7
更新日期：2002-11-15 00:00:00
abstract：:Superpositioning of multiple 3-dimensional digital volumes is essential to extending neuroimaging capabilities from single-subject analysis to group evaluation. In this study, 6 rat heads were cryoplaned and digital images of the specimen blockface were organized into data volumes. The midline plane of each brain guid...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90007-e
更新日期：1993-11-01 00:00:00
abstract：:Since transgenes were first cloned into recombinant adenoviruses almost 30 years ago, a variety of viral vectors have become important tools in genetic research. Viruses adeptly transport genetic material into eukaryotic cells, and replacing all or part of the viral genome with genes of interest or silencing sequences...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.02.026
更新日期：2011-04-30 00:00:00
abstract：BACKGROUND:Advancement in clinically relevant studies like seizure interruption using functional neuro imaging tools has shown that specific changes in hemodynamics precede and accompany seizure onset and propagation. However, preclinical seizure experiments need to be conducted in awake animals with images reconstruct...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.10.018
更新日期：2015-01-30 00:00:00
abstract：:So far, the double-magnetic induction (DMI) method has been successfully applied to record eye movements from head-restrained humans, monkeys and cats. An advantage of the DMI method, compared to the more widely used scleral search coil technique, is the absence of vulnerable lead wires on the eye. A disadvantage, how...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.08.012
更新日期：2007-02-15 00:00:00
abstract：:Viable neurons in brain slices are crucial for electrophysiological studies. The present study describes a new method for obtaining viable cells in several regions of the central nervous system including the ventral tegmental area, the hypothalamus, the periaqueductal grey matter and the spinal cord. The essence of th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.06.006
更新日期：2006-12-15 00:00:00
abstract：:A novel test for the selective identification of anxiolytic and anxiogenic drug effects in the rat is described, using an elevated + -maze consisting of two open arms and two enclosed arms. The use of this test for detecting such drug effects was validated behaviourally, physiologically, and pharmacologically. Rats ma...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90031-7
更新日期：1985-08-01 00:00:00
abstract：:The paired motor unit analysis provides in vivo estimates of the magnitude of persistent inward currents (PIC) in human motoneurons by quantifying changes in the firing rate (ΔF) of an earlier recruited (reference) motor unit at the time of recruitment and derecruitment of a later recruited (test) motor unit. This stu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.03.018
更新日期：2011-05-15 00:00:00
abstract：BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.015
更新日期：2015-05-15 00:00:00
abstract：:Analysis of the antigenic components of synaptic membranes presents several problems caused by detergents required to solubilize the proteins. These problems involve detection of the reaction between antibodies and proteins in the detergent extract as well as characterization of the individual antigens after further p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90058-8
更新日期：1980-04-01 00:00:00
abstract：:The most common behavioural disorder seen in children and adolescents born extremely prematurely is attention deficit hyperactivity disorder (ADHD). The hyperactive/impulsive sub-type of ADHD or the inattentive sub-type or the hyperactive/impulsive/inattentive sub-type can be evident. These sub-types of ADHD can persi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.01.010
更新日期：2007-11-30 00:00:00
abstract：:Chimera construction between different proteins is a useful method for investigating protein structure and function relationships. However, this technique, by its traditional application, has been daunting because of its complex procedure and low success rate. Here we describe a protocol for constructing chimeras betw...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.08.033
更新日期：2010-10-30 00:00:00
abstract：:A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00207-w
更新日期：1995-07-01 00:00:00
abstract：BACKGROUND:Fear conditioning (FC) in rodents is the most used animal model to investigate the neurobiology of posttraumatic stress disorder (PTSD). Although research using FC has generated a better understanding of fear memories, studies often rely on mild or moderate FC training and behavioral analysis generally focus...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.004
更新日期：2018-03-15 00:00:00
abstract：:A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90062-8
更新日期：1987-08-01 00:00:00
abstract：:The use of c-fos protein (Fos) immunocytochemistry as a metabolic marker for tracing neuroanatomical connections, seizure pathways and sites of action of neuroactive drugs is discussed in this report. Fos immunocytochemistry will be very useful for these purposes providing that a number of potential problems are recog...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(89)90150-7
更新日期：1989-09-01 00:00:00
abstract：:Binding assays for the norepinephrine (NE) transporter (NET) with [3H]nisoxetine have generally yielded weak potencies for compounds related to cocaine and 1-(2-(di(4-fluorophenyl)-methoxy)-ethyl)-4-(3-phenylpropyl)piperazine (GBR 12909), as compared with their functional activity in inhibiting NE uptake. In the prese...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.09.015
更新日期：2005-04-15 00:00:00
abstract：:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90048-i
更新日期：1992-10-01 00:00:00
abstract：:1321N1 astrocytoma cells are frequently used to analyze stimulus-induced intracellular signaling. These experiments require genetic manipulation of the cells and several chemical and physical methods have been employed in the past. Recently, microporation has been suggested as the best method to transfect 1321N1 astro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.02.016
更新日期：2012-01-01 00:00:00
abstract：:Microglia are brain cells of considerable interest because of their role in CNS inflammatory responses and strong association with neuritic plaques in Alzheimer's disease (AD). In the present study, immunocytochemistry was combined with unbiased stereology to estimate the mean total number of microglia in dentate gyru...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00100-9
更新日期：1998-10-01 00:00:00
