Abstract:
:We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a pneumatic tourniquet to the base of the tail until the rats exhibited escape behaviour. Control animals had a sham tourniquet placed on their tails. During tail reperfusion, there was significant hyperalgesia when the noxious mechanical stimulus was applied by a narrow bar transverse to the tail, less when applied by a rubber piston, and there was no hyperalgesia when the stimulus was punctate, similar to a von Frey hair. Repeated application of the mechanical stimuli themselves, at 30-min intervals, did not induce hyperalgesia. Rats subjected repetitively to the combination of the bar algometer and tail ischaemia at intervals of at least 48 h, for a total period of three weeks, exhibited progressively enhanced hyperalgesia to the bar algometer, and also enhanced hyperalgesia to noxious ischaemic and thermal stimuli. Rats therefore do exhibit hyperalgesia to mechanical stimuli following conditioning ischaemia, but the nature of the hyperalgesia depends critically on the geometry of the algometer, and the time course of the hyperalgesia was different to that which manifests to a noxious thermal stimulus.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Vidulich L,Mitchell Ddoi
10.1016/s0165-0270(00)00313-7subject
Has Abstractpub_date
2000-11-30 00:00:00pages
173-80issue
2eissn
0165-0270issn
1872-678Xpii
S0165027000003137journal_volume
103pub_type
杂志文章abstract::Electrical neural stimulation is the technique used to modulate neural activity by inducing an instantaneous charge imbalance. This is typically achieved by injecting a constant current and controlling the stimulation time. However, constant voltage stimulation is found to be more energy-efficient although it is chall...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.11.028
更新日期:2014-03-15 00:00:00
abstract::The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of th...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00144-1
更新日期:1997-12-30 00:00:00
abstract::In the present study the accumulation of protease resistant prion protein (PrPres) in scrapie-infected neuroblastoma cells (ScN2a cells) was shown to be dependent on culture conditions. The highest levels of PrPres were found in slow growing cells. Further increases in PrPres accumulation were observed in ScN2a cells ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.04.001
更新日期:2004-09-30 00:00:00
abstract::Images of Golgi impregnated neurons from different laminae of the human and rat dorsal horns were subjected to a quantitative analysis to support the Rexed's laminar scheme in mammals. Four methods of fractal analysis were performed in the proceedings: box-counting, mass-radius, cumulative intersection, and vectorized...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.02.009
更新日期:2005-08-15 00:00:00
abstract:BACKGROUND:Currently the problem of incorporating priori information into an independent component analysis (ICA) model is often solved under the framework of constrained ICA, which utilizes the priori information as a reference signal to form a constraint condition and then introduce it into classical ICA. However, it...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.03.018
更新日期:2017-05-01 00:00:00
abstract::Maintaining a stable physiologic state is essential when studying animal models of epilepsy with simultaneous electroencephalograph (EEG) and functional magnetic resonance imaging (fMRI) or EEG and magnetic resonance spectroscopy (MRS). To achieve and maintain such stability in rats in the MRI environment, a minimally...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.11.019
更新日期:2005-06-15 00:00:00
abstract::Studies of the neural control of movement often require or benefit from long-term recording of EMG activity from large numbers of muscles involved in a particular motor task. While chronic recording of EMG activity has been described in a number of previous monkey studies, the number of muscles recorded has been somew...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00155-2
更新日期:2000-03-15 00:00:00
abstract::Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultur...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00025-8
更新日期:1996-10-21 00:00:00
abstract::A simple and low-cost device is described which facilitates the calibration of intracellular DC recordings in amplitude and time. A current source, optically coupled to a conventional stimulator, injects a pulse of current, across a 1-omega resistor connecting the preparation to ground. Amplitude and duration of the c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(84)90008-6
更新日期:1984-05-01 00:00:00
abstract::Inefficiency in primary neural stem cell transfection is a major obstacle in gene expression research aimed at determining the mechanisms underlying brain development. Following our initial finding that liposome-mediated transfection procedures are fairly toxic to neural stem cells, we further examined whether transfe...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00090-0
更新日期:2002-06-30 00:00:00
abstract::The object-oriented graphical programming language LabView was used to implement the numerical solution to a computational model of saccade generation in primates. The computational model simulates the activity and connectivity of anatomical strictures known to be involved in saccadic eye movements. The LabView progra...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00171-x
更新日期:1999-03-01 00:00:00
abstract::The aim of this work is to define neurotoxins doses to have efficient and specific depletion of noradrenaline (NA), serotonin (5-HT) neurotransmission in cortex, striatum, hippocampus and hypothalamus of Swiss mice after intraperitoneal administration of, respectively, N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.06.008
更新日期:2006-01-15 00:00:00
abstract::Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(96)00072-6
更新日期:1996-08-01 00:00:00
abstract::An encephalomyosynangiosis (EMS) is a temporal muscle graft that is placed onto the surface of the brain to serve as a source for collateral vessel growth for brain revascularization in patients with Moyamoya Disease (MMD). To facilitate an EMS in patients with occlusive cerebrovascular diseases other than MMD, the tr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.07.011
更新日期:2011-09-30 00:00:00
abstract::A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90045-3
更新日期:1988-01-01 00:00:00
abstract::We describe a simple, scaled-down instrument which enables accurate, reproducible stereotaxic placements into specific sites in the brain of the newborn rat. The instrument is specially designed for the administration of long-term hypothermia, yet permits the use of alternative methods of anesthesia. The design of the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90026-n
更新日期:1993-04-01 00:00:00
abstract:BACKGROUND:Motion-onset visual evoked potentials (mVEP) can provide a softer stimulus with reduced fatigue, and it has potential applications for brain computer interface(BCI)systems. However, the mVEP waveform is seriously masked in the strong background EEG activities, and an effective approach is needed to extract t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.11.002
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Emotion recognition plays a key role in multimedia. To enhance the sensation of reality, smell has been incorporated into multimedia systems because it can directly stimulate memories and trigger strong emotions. NEW METHOD:For the recognition of olfactory-induced emotions, this study explored a combination...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108599
更新日期:2020-01-21 00:00:00
abstract:INTRODUCTION:Our aim was to assess differences in movement measures in attention-deficit/hyperactivity disorder (ADHD) vs. typically developing (TD) controls. METHODS:We performed meta-analyses of published studies on motion measures contrasting ADHD with controls. We also conducted a case-control study with children/...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,meta分析
doi:10.1016/j.jneumeth.2015.03.001
更新日期:2015-08-30 00:00:00
abstract::Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not s...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.007
更新日期:2008-08-30 00:00:00
abstract::Functional magnetic resonance imaging (fMRI) based on BOLD signal has been used to indirectly measure the local neural activity induced by cognitive tasks or stimulation. Most fMRI data analysis is carried out using the general linear model (GLM), a statistical approach which predicts the changes in the observed BOLD ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.09.005
更新日期:2011-01-15 00:00:00
abstract::A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.09.016
更新日期:2010-12-15 00:00:00
abstract::A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90106-1
更新日期:1983-09-01 00:00:00
abstract:BACKGROUND:To increase power when analyzing fMRI data, researchers often define functional regions of interest (fROIs). It is crucial that this fROI is defined with an optimal balance between both false positives and false negatives to ensure maximal spatial accuracy and to avoid potentially biased results in the main ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.108417
更新日期:2020-01-15 00:00:00
abstract::Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00133-6
更新日期:1996-02-01 00:00:00
abstract::In order to optimize a method for quantitative assessment of bradykinesia, we evaluated the three-dimensional sources of a movement signal of the wrist and influence of tremor on the reliability of bradykinesia measurements. A total of 33 patients with Parkinson's disease, three patients with Multiple System Atrophy a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)02254-1
更新日期:1997-06-06 00:00:00
abstract::Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(98)00057-0
更新日期:1998-08-01 00:00:00
abstract:BACKGROUND:This article addresses the automatic classification of reconstructed neurons through their morphological features. The purpose was to extend the capabilities of the L-Measure software. METHODS:New morphological features were developed, based on modifications of the conventional Sholl analysis. The lengths o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108835
更新日期:2020-09-01 00:00:00
abstract::Here we describe a method for intracellularly injecting mixtures of the fluorescent dye Lucifer Yellow and the permanent tracers HRP or biocytin into aldehyde-fixed slices of the dorsal lateral geniculate nucleus in young postnatal cats. Lucifer Yellow was used for visual control in the injection procedure and the inc...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(92)90009-3
更新日期:1992-09-01 00:00:00
abstract:BACKGROUND:Local shape complexity can be biologically meaningful as a marker of disease, trauma, or change in brain structure over time. Fractal dimensionality (FD) is currently the dominant measure of local shape complexity used in neuroimaging but its limitations are not well understood. NEW METHOD:Elliptical Fourie...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.05.009
更新日期:2019-07-15 00:00:00