Abstract：
:In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Immunoreactive TH on PVDF membranes was identified with optimized concentrations of TH antibody followed by application of [125I]labeled secondary antibody. Quantities of TH on autoradiograms were estimated by comparing optical densities of transferred immunoblots to a calibrated standard curve produced with purified recombinant TH dotted onto the same PVDF membranes. Amounts of TH-immunoreactivity in the LC were proportional to the thickness of tissue sections up to 15 micrometer. However, the amounts of total protein, as measured by Ponceau S staining, were linearly related to section thicknesses up to 30 micrometer. Comparisons of quantities of immunoreactive TH in the LC using this method to amounts determined using traditional Western blotting, in which LC tissue was punched from adjacent sections from the same subject, showed a positive correlation (r(2)=0.99, P<0.01). Using the transfer immunoblot method, an uneven distribution of TH protein was observed along the rostrocaudal axis of the human LC (P<0.01). This method may provide a sensitive and useful tool for the study of the role of human TH expression in the pathophysiology of psychiatric disease.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Zhu M,Klimek V,Haycock JW,Ordway GAdoi
10.1016/s0165-0270(00)00211-9subject
Has Abstractpub_date
2000-06-30 00:00:00pages
37-44issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165027000002119journal_volume
99pub_type
杂志文章abstract：:The most direct evaluation of human brain activity has been obtained from intracranial electrodes placed either on the surface of the brain or inserted into the brain to record from deep brain structures. Currently, the placement of intracranial electrodes implies transcranial surgery, either through a burr hole or a ...
journal_title：Journal of neuroscience methods
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abstract：:Evaluation of electron microscopic images of serial sections is a time-consuming process requiring a high level of expertise. Here we present an algorithm to ease and accelerate this process. It is a modification of an algorithm successfully used in computer vision for object recognition. However, rather than recognis...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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abstract：BACKGROUND:Neuronal cell cultures are widely used in the field of neuroscience. Cell dissociation allows for the isolation of a desired cell type, yet the neuronal complexity that distinguishes the nervous system is often lost as a result. Thus, culturing neural tissues in ex vivo format provides a physiological contex...
journal_title：Journal of neuroscience methods
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abstract：:Holding a slipping object in hand requires adjustment of grip forces. The aim of the study was to develop a method for measuring the temporal and spatial distribution of grip forces during the holding of a slipping object in the hand. A special grip rod with a measuring film containing 200 resistor-based pressure sens...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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更新日期：2011-01-30 00:00:00
abstract：:Phalen's test, with more than 100 degrees of elbow flexion, is not always comfortable for patients with elbow pathology. Therefore, we suggest a modification of the test with the patient having both arms resting on a pillow or arm-holders in a relaxed position, the hands floating at the end, and the examiner passively...
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更新日期：2008-05-15 00:00:00
