Abstract：
:Evaluation of electron microscopic images of serial sections is a time-consuming process requiring a high level of expertise. Here we present an algorithm to ease and accelerate this process. It is a modification of an algorithm successfully used in computer vision for object recognition. However, rather than recognising individual structures, we estimate the spatial mapping of a whole section onto the consecutive one. This mapping is used to transfer labelled information of the very first section, e.g. a classification by a human expert of different visible structures, onto structures visible in the next section. We investigate its performance on an artificially constructed benchmark as well as on real electron microscopic samples taken in primary visual cortex and demonstrate its potential for dramatically facilitating the evaluation process of serial sections.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
König P,Kayser C,Bonin V,Würtz RPdoi
10.1016/s0165-0270(01)00439-3subject
Has Abstractpub_date
2001-10-30 00:00:00pages
141-50issue
2eissn
0165-0270issn
1872-678Xpii
S0165027001004393journal_volume
111pub_type
杂志文章abstract：:Microwire array electrodes are important in multi-site, multiple single-unit recording experiments. A simple method is described herein for the construction of microwire arrays consisting of evenly spaced insulated microwires in either horizontal or vertical orientations. Several key steps in the fabrication of a good...
journal_title：Journal of neuroscience methods
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abstract：:A method which improves carbon fiber microelectrode selectivity for cationic amines by electrocoating the fiber with a thin film of the ionic polymer, Nafion, is described. The selectivity and response speed of these electrodes for the detection of electroactive cationic and anionic species found in brain extracellula...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90011-2
更新日期：1987-12-01 00:00:00
abstract：:Combining multiple linear univariate features in one feature space and classifying the feature space using machine learning methods could predict epileptic seizures in patients suffering from refractory epilepsy. For each patient, a set of twenty-two linear univariate features were extracted from 6 electroencephalogra...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.03.019
更新日期：2013-07-15 00:00:00
abstract：:We describe a method for estimating the average proportion of GABAergic terminal area relative to total nerve terminal area with confocal microscopy. Nerve terminal regions were identified with dual colour immunofluorescence on Vibratome sections with an antibody to synaptophysin (SYN), and GABAergic processes, includ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00163-6
更新日期：2000-01-31 00:00:00
abstract：:We characterised the dynamics in the oscillatory potentials (OPs) of the rat electroretinogram (ERG) using a continuous complex Morlet wavelet transform. Dark-adapted (>12h) full field ERG responses were recorded from five anaesthetized (ketamine:xylazine, 60:5mg/kg) adult Long-Evans rats (10-12weeks). Five responses ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.007
更新日期：2008-03-30 00:00:00
abstract：:Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00015-8
更新日期：2002-04-15 00:00:00
abstract：:We describe a novel procedure for combining immunocytochemistry with in situ hybridisation. In contrast to previously published procedures, the technique involves immunofluorescence followed by in situ hybridization and is particularly suitable for antigens which are labile or sensitive to in situ hybridization proces...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(05)80011-1
更新日期：1993-06-01 00:00:00
abstract：:Hydraulically coupled volume microejection (HCVM) was employed to overcome known drawbacks of conventional pressure ejection of drugs in isolated brain slices and other tissues. For HCVM, a solution-filled glass micropipette is connected to a motor-driven, liquid-filled syringe by low-compliance, liquid-filled tubing,...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.01.007
更新日期：2006-09-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.013
更新日期：2008-05-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.08.022
更新日期：2014-11-30 00:00:00
abstract：:This paper presents a technique for stimulating and recording from multiple intact spinal roots in the in vitro mudpuppy (Necturus maculatus) spinal cord-forearm preparation using fine wire electrodes, a modified intrafascicular electrode. We found that multiple spinal roots of the preparation could be implanted with ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00176-4
更新日期：2000-03-01 00:00:00
abstract：BACKGROUND:Deviant stimuli within a standard, frequent stimulus train induce a cortical somatosensory mismatch response (SMMR). The SMMR reflects the brain's automatic mechanism for the detection of change in a somatosensory domain. It is usually elicited by electrical stimulation, which activates nerve fibers and rece...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.09.014
更新日期：2019-01-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90043-6
更新日期：1980-02-01 00:00:00
abstract：:Children and adolescents with attention-deficit/hyperactivity disorder (ADHD) have smaller cerebellar volumes, particularly in the posterior-inferior cerebellar vermis (lobules VIII-X). Functional activation of the human cerebellar vermis following stimulant administration has also been repeatedly demonstrated. There ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.09.019
更新日期：2006-02-15 00:00:00
abstract：BACKGROUND:Functional Magnetic Resonance Imaging (fMRI) is used for exploring brain functionality, and recently it was applied for mapping the brain connection patterns. To give a meaningful neurobiological interpretation to the connectivity network, it is fundamental to properly define the network framework. In partic...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.03.004
更新日期：2014-05-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.02.016
更新日期：2012-01-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.06.013
更新日期：2017-08-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.01.013
更新日期：2019-04-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90076-7
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00043-2
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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更新日期：1999-02-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90036-6
更新日期：1985-08-01 00:00:00
abstract：:The reproducibility of motor unit twitches obtained using spike-triggered averaging (STA) was examined in the human first dorsal interosseus. For each motor unit (30 total) a series of STA twitches was derived using a 30 s averaging window. Within each averaging window, eight independent measures characterizing motor ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00077-3
更新日期：2003-06-30 00:00:00
abstract：:A method by which muscles, nerves, and a section of spinal cord from a turtle can be stably maintained in vitro for many days has been developed. Tests of the viability of central and neuromuscular synapses, and the histochemical properties of muscle fibers have indicated that the preparation is viable and shows littl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90029-0
更新日期：1989-08-01 00:00:00
abstract：:We describe a semi-automated tracking system for insect motion based on commercially available high-speed video cameras and freely available software. We use it to collect detailed three-dimensional kinematic information from female crickets performing free walking phonotaxis towards a calling song stimulus. We mark t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.011
更新日期：2012-01-15 00:00:00
abstract：BACKGROUND:Motion-onset visual evoked potentials (mVEP) can provide a softer stimulus with reduced fatigue, and it has potential applications for brain computer interface(BCI)systems. However, the mVEP waveform is seriously masked in the strong background EEG activities, and an effective approach is needed to extract t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.11.002
更新日期：2017-01-01 00:00:00
abstract：BACKGROUND:The complexity of analyzing eye-tracking signals increases as eye-trackers become more mobile. The signals from a mobile eye-tracker are recorded in relation to the head coordinate system and when the head and body move, the recorded eye-tracking signal is influenced by these movements, which render the subs...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.09.005
更新日期：2016-12-01 00:00:00
abstract：:A novel epineural tube implantation paradigm in the adult rat was designed for the analysis of regulatory cell interactions in peripheral nerves and for the development of therapeutic implants. The aim was to allow the integration of synthetic regenerative structures and cells into the nerve interior while preserving ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.04.007
更新日期：2010-06-15 00:00:00
abstract：:The assumptions upon which a correct interpretation of the t-test depends are rarely fulfilled by data from the neurosciences. This applies to both independent and correlated samples. The Mann-Whitney U-test is suggested as an alternative for the t-test for independent samples. The way in which significant results fro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(83)90058-4
更新日期：1983-12-01 00:00:00
