Abstract：
:We characterised the dynamics in the oscillatory potentials (OPs) of the rat electroretinogram (ERG) using a continuous complex Morlet wavelet transform. Dark-adapted (>12h) full field ERG responses were recorded from five anaesthetized (ketamine:xylazine, 60:5mg/kg) adult Long-Evans rats (10-12weeks). Five responses were obtained for brief LED flashes (1-4ms) in a ganzfeld at exposures ranging from -4.2 to 1.58logcdsm(-2). Signals were recorded across a bandwidth of 0.3-1kHz and digitized at 10kHz. Morlet wavelets with frequencies between 50 and 250Hz were correlated with raw ERG signals at 1ms intervals. The amplitude of the correlation at each time and frequency was given by the modulus of the complex wavelet response. Candidate OPs were identified as local peaks within 10% of the maximum amplitude. As flash exposure increased, the amplitude of the OP response increased, the peak OP occurred earlier, and the peak OP frequency increased. OPs at brighter flashes clustered into two groups, peaking at 50ms in the 70 and 130Hz band for moderate intensities, and peaking at 20ms in the 70Hz band and 50ms in the 120Hz band for the highest intensities (>0logcdsm(-2)). These dynamics agree with physiological, pharmacological and clinical studies that suggest several distinct neural mechanisms contribute to OPs. Wavelet analysis reveals important dynamics in OP data that are not evident with traditional analytical approaches.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Forte JD,Bui BV,Vingrys AJdoi
10.1016/j.jneumeth.2007.12.007subject
Has Abstractpub_date
2008-03-30 00:00:00pages
191-200issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(07)00610-3journal_volume
169pub_type
杂志文章abstract：:Glass plates coated with transparent thin film conductors of indium-tin oxide (ITO), 100 nm thick and 10 microns wide, have been successfully used to record spike potentials from neuronal monolayer cultures. The material is non-toxic to mammalian spinal neurons and is stable under warm culture medium. Laser-deinsulate...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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