Abstract：
:The use of a devitalized skeletal muscle graft and conventional nerve graft to repair a 5mm long segmentary sciatic nerve lesion was studied in rats by means of functional, morphometric and spinal cord motor neuron cell response evaluation. Thirty-four rats were used and divided into four groups according to the procedure: (1) sham operation; (2) conventional nerve grafting; (3) muscle grafting; (4) unrepaired lesion. The sciatic functional index (SFI) was evaluated every fortnight up to the 105th postoperative day by measuring three parameters in the rats' footprint. The animals of Groups 2 and 3 presented initial complete functional loss, followed by slow but steady recovery, with final similar SFIs. The histologic and morphometric studies showed an increased small diameter/thin myelin sheath nerve fiber density distally to the lesion site for both types of graft. An increased population of motor neurons was observed in the anterior horn of the lumbar spinal cord segment with both types of grafts, but not in the control groups. The SFI, histologic and morphometric data did not differ significantly between the two types of graft, thus indicating a similar behavior. The authors conclude that a 5mm long skeletal muscle graft works as well as a conventional nerve graft.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Oliveira EF,Mazzer N,Barbieri CH,DelBel EAdoi
10.1016/j.jneumeth.2003.09.010subject
Has Abstractpub_date
2004-02-15 00:00:00pages
19-26issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165027003003078journal_volume
133pub_type
杂志文章abstract：:It is often helpful to display analogue signals such as electromyograms, electroencephalograms, nerve action potentials, etc., in a regular vertical array. A special case of this display is the widely-used dot raster. This paper describes a simple circuit which enables up to 31 analogue signals to be thus displayed on...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90009-9
更新日期：1986-03-01 00:00:00
abstract：BACKGROUND:This study analyses multiple spike trains (MST) data, defines its functional connectivity and subsequently visualises an accurate diagram of connections. This is a challenging problem. For example, it is difficult to distinguish the common input and the direct functional connection of two spike trains. NEW ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.05.016
更新日期：2017-07-15 00:00:00
abstract：:This paper overviews the design and implementation of three neuromorphic integrated circuits developed for the COLAMN ("Novel Computing Architecture for Cognitive Systems based on the Laminar Microcircuitry of the Neocortex") project. The circuits are implemented in a standard 0.35 μm CMOS technology and include spiki...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.01.019
更新日期：2012-09-15 00:00:00
abstract：BACKGROUND:How experience and individuality shape action selection remains a major question in neuroscience. Visually-evoked escape behavior within Drosophila melanogaster provides a robust model to study these mechanisms within neural circuits but requires novel assays to circumvent limitations of current behavior ass...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.04.019
更新日期：2018-07-01 00:00:00
abstract：:Three-dimensional (3D) reconstruction from serial sections that are undistorted and true-to-life is only possible when a reference system exists that allows correct matching of the section drawings. This paper describes a simple method for producing reference lines that ensures proper alignment of the drawings. (1) Th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90162-8
更新日期：1988-12-01 00:00:00
abstract：:We have developed a superfusion method utilizing an open-volume microfluidic device for administration of pharmacologically active substances to selected areas in brain slices with high spatio-temporal resolution. The method consists of a hydrodynamically confined flow of the active chemical compound, which locally st...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.08.012
更新日期：2013-10-15 00:00:00
abstract：:In the last few years quite powerful complete computer systems have become available that fill many laboratory needs at low cost (US $3000-10,000). This development is based on the use of microcomputers, which now have capabilities that were previously associated with minicomputers. Due to the rapidly expanding use of...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(82)90044-9
更新日期：1982-01-01 00:00:00
abstract：BACKGROUND:Single unit recording in behaving nonhuman primates is widely used to study the primate central nervous system. However, certain questions cannot be addressed without recording large numbers of neurons simultaneously. Multiple 96-electrode probes can be implanted at one time, but certain problems must be ove...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.07.001
更新日期：2017-09-01 00:00:00
abstract：:The event-related potential (ERP) and event-related field (ERF) techniques provide valuable insights into the time course of processes in the brain. Because neural signals are typically weak, researchers commonly filter the data to increase the signal-to-noise ratio. However, filtering may distort the data, leading to...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.06.011
更新日期：2012-07-30 00:00:00
abstract：:A discontinuous gradient of Percoll has been used to prepare a subcellular fraction (fraction III) from fish brain which is greatly enriched in synaptic nerve endings (synaptosomes). Synaptosomes display well-preserved morphology, good functional performance as assessed by ability to release preloaded neurotransmitter...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00137-9
更新日期：1997-02-01 00:00:00
abstract：:A biotinylated lectin from Ricinus communis (RCA-I) and avidin-biotin-horseradish peroxidase complex (ABC) were used to identify microglial cells in 3-microns-thick sections of formalin-fixed paraffin-embedded brains of adult mice required for quantitative cell kinetic studies. In 3-microns-thick sections of the mouse...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90034-o
更新日期：1993-12-01 00:00:00
abstract：:Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be use...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.11.010
更新日期：2010-02-15 00:00:00
abstract：:The development and effective use of robust high-electrode-count microelectrode arrays for neuronal recording and stimulation depends on effective monitoring of electrode impedances and how these change over time. In multielectrode arrays, conventional electrode impedance measurements may be confounded by possible shu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.12.020
更新日期：2009-04-15 00:00:00
abstract：BACKGROUND:Flow cytometry is an efficient and powerful technique to characterize and quantify numerous cells. However, the strengths of this technique have not been widely harnessed in neurosciences due to the critical step of CNS tissue preparation into a single cell suspension. Previous reports assessed either neural...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.021
更新日期：2015-05-30 00:00:00
abstract：:The paper presents in detail a method for approximating the spatial position of neural spike activity from tetrode recordings. The method uses a nonlinear mapping of a set of tetrode tip amplitudes into three-dimensional (3D) space, followed by a self-organizing map clustering technique. Viewed as a spike sorting meth...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.09.004
更新日期：2005-03-30 00:00:00
abstract：:Mitochondrial defects have been implicated in the degeneration of axons in a number of CNS disorders, including multiple sclerosis. Uniquely, mitochondria harbor the only non-nuclear DNA (mitochondrial DNA or mtDNA), which encodes functionally important subunits of the respiratory chain. The pattern of mitochondrial r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.07.022
更新日期：2010-09-30 00:00:00
abstract：BACKGROUND:Dilated brain perivascular spaces (PVSs) are found to be associated with many conditions, including aging, dementia, and Alzheimer's disease (AD). Conventionally, PVS assessment is mainly based on subjective observations of the number, size and shape of PVSs in MR images collected at clinical field strengths...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.09.001
更新日期：2015-12-30 00:00:00
abstract：:The technique named detrended fluctuation analysis (DFA) has been used to reveal the presence of long-range temporal correlations (LRTC) and scaling behavior (SB) in electroencephalographic (EEG) recordings. The occurrence of these phenomena seems to be a salient characteristic of the healthy human brain and alteratio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.010
更新日期：2008-05-30 00:00:00
abstract：:Amperometry is a very powerful technique for investigating the role(s) specific proteins play in exocytosis at the single-cell level. In this study, amperometry has been used to investigate possible changes in exocytosis at chromaffin cells isolated from coloboma and tottering mutant mice. Coloboma mice possess a dele...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00359-9
更新日期：2001-01-30 00:00:00
abstract：:MicroRNAs (miRNAs) finely tune messenger RNA (mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei. MiRNA expression profiling from individual samples is also important for investigating potential dif...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.033
更新日期：2012-07-30 00:00:00
abstract：BACKGROUND:Brain parcellation is important for exploiting neuroimaging data. Variability in physiology between individuals has led to the need for data-driven approaches to parcellation, with recent research focusing on simultaneously estimating and partitioning the network structure of the brain. NEW METHOD:We view d...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108628
更新日期：2020-04-01 00:00:00
abstract：:Detecting brain structural changes from magnetic resonance (MR) images can facilitate early diagnosis and treatment of neurological and psychiatric diseases. Many existing methods require an accurate deformation registration, which is difficult to achieve and therefore prevents them from obtaining high accuracy. We de...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.001
更新日期：2014-01-15 00:00:00
abstract：:We have developed a quantitative reverse transcription polymerase chain reaction (RT-PCR) method, using both internal and external standardisation, to quantitate fg amounts of pre-pro-thyrotropin releasing hormone (pre-pro-TRH) mRNA in the paraventricular nucleus (PVN) of individual laboratory rats. A constant amount ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00090-8
更新日期：1996-10-01 00:00:00
abstract：BACKGROUND:Despite extensive environmental standardization and the use of genetically and microbiologically defined mice of similar age and sex, individuals of the same mouse inbred strain commonly differ in quantitative traits. This is a major issue as it affects the quality of experimental results. Standard analysis ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108810
更新日期：2020-09-01 00:00:00
abstract：:The whole cell patch clamp technique allows recording of membrane currents in an entire cell under voltage clamp conditions. However, technical difficulties arise in large cells bearing extensive processes, such as human fetal dorsal root ganglion (DRG) neurons in culture. In order to improve space clamp conditions, h...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90094-v
更新日期：1990-10-01 00:00:00
abstract：:This paper describes improvements and further characterization of a ceramic-based multisite microelectrode for in vivo measurements of L-glutamate. Improvements include increased recording area, insulation deposition using photolithography for more uniform recording sites and forming the microelectrodes using a diamon...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00172-3
更新日期：2002-09-30 00:00:00
abstract：:Bone morphogenetic protein 7 (BMP7), a member of the transforming growth factor β (TGF-β) superfamily, plays important roles in the development of various tissues and organs in mouse and human. In particular, BMP7 is critical for the formation of the nervous system and it is considered to have therapeutic potential in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.12.007
更新日期：2012-03-30 00:00:00
abstract：:Songbirds are the preeminent animal model for understanding how the brain encodes and produces learned vocalizations. Here, we report a new statistical method, the Kullback-Leibler (K-L) distance, for analyzing vocal change over time. First, we use a computerized recording system to capture all song syllables produced...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.06.033
更新日期：2008-09-15 00:00:00
abstract：BACKGROUND:In clinical practice, small myelinated sensory fibers, Aδ-fibers, conveying mainly pain and temperature sensations, cannot be examined with available nerve conduction study techniques. Currently, these fibers can only be examined with experimental or very specialized and not commonly available nerve conducti...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109045
更新日期：2020-12-24 00:00:00
abstract：:A semi-automated analysis system based on video image analysis was developed to count labelled and unlabelled nuclei of Schwann cells which had been exposed to tritiated thymidine followed by processing for autoradiography. A Model 3000 Image Analysis system (Image Technology Corporation, Deer Park, NY) was used to ac...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90062-8
更新日期：1987-08-01 00:00:00
