Abstract：
:Amperometry is a very powerful technique for investigating the role(s) specific proteins play in exocytosis at the single-cell level. In this study, amperometry has been used to investigate possible changes in exocytosis at chromaffin cells isolated from coloboma and tottering mutant mice. Coloboma mice possess a deletion mutation that encompasses the gene for the presynaptic protein SNAP-25 and tottering mice carry a mutation of the alpha(1A) subunit gene, which encodes the pore-forming region of P/Q-type calcium channels. Although amperometric data measured from tottering and coloboma cells are not significantly different from that measured at wild-type control cells, significant differences are found when groups of wild-type chromaffin cells are analyzed at room temperature and at 37 degrees C. Due to the large variability inherent to amperometric data, it is possible that changes in release resulting from some genetic differences cannot be detected. To fully exploit the technical advantages of using mouse chromaffin cells, experimental guidelines are described which should maximize changes in release resulting from genetic differences and increase the likelihood of detecting a change in amperometric data.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Colliver TL,Hess EJ,Ewing AGdoi
10.1016/s0165-0270(00)00359-9subject
Has Abstractpub_date
2001-01-30 00:00:00pages
95-103issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(00)00359-9journal_volume
105pub_type
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