Abstract:
BACKGROUND:How experience and individuality shape action selection remains a major question in neuroscience. Visually-evoked escape behavior within Drosophila melanogaster provides a robust model to study these mechanisms within neural circuits but requires novel assays to circumvent limitations of current behavior assays. METHOD:Here we describe and characterize a simple, low to moderate cost, and flexible assay for studying visually-evoked escape responses in tethered flies. This assay consists of a DLP projector, cylindrical rear projection screen, and an automated flight interruption motor all controlled within a MATLAB environment. RESULTS:We find this assay effectively recapitulates fly behaviors previously observed in free behavior assays, and provides a novel opportunity to investigate the behavior of individual flies over the course of numerous stimulus presentations. COMPARISON TO EXISTING METHODS:Current Drosophila escape assays do not permit multiple stimulus presentations and can be highly complex and expensive to implement. CONCLUSIONS:This assay provides an effective system to further identify neural components and mechanisms underlying action selection within parallel sensorimotor pathways.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Goodman DP,Eldredge A,von Reyn CRdoi
10.1016/j.jneumeth.2018.04.019subject
Has Abstractpub_date
2018-07-01 00:00:00pages
154-161eissn
0165-0270issn
1872-678Xpii
S0165-0270(18)30128-6journal_volume
304pub_type
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